Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0992 and PP_0993 are separated by 16 nucleotidesPP_0993 and PP_0994 are separated by 271 nucleotidesPP_0994 and PP_0995 overlap by 11 nucleotidesPP_0995 and PP_0996 are separated by 77 nucleotides PP_0992: PP_0992 - conserved protein of unknown function, UPF0276 family, at 1,131,479 to 1,132,312 _0992 PP_0993: PP_0993 - conserved exported protein of unknown function, at 1,132,329 to 1,132,616 _0993 PP_0994: PP_0994 - putative RNA polymerase sigma factor, at 1,132,888 to 1,133,424 _0994 PP_0995: PP_0995 - conserved membrane protein of unknown function, at 1,133,414 to 1,134,055 _0995 PP_0996: PP_0996 - glutamate:sodium symporter, at 1,134,133 to 1,135,338 _0996
Group Condition PP_0992 PP_0993 PP_0994 PP_0995 PP_0996
carbon source 4-Hydroxyvalerate (C) (40mM) -0.1 N.D. +0.2 -3.0 +0.0
phage P. putida Sci Big MOI 0.1 -1.3 N.D. -0.4 -0.8 -0.3
carbon source 4-Hydroxyvalerate (C) (40mM) -0.1 N.D. +0.0 -2.5 -0.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.2 N.D. -0.6 -2.0 -0.2
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.1 N.D. -0.1 -2.4 +0.1
nitrogen source Hippuric (N) -0.2 N.D. -0.4 -1.9 +0.2
phage P. putida Sci small MOI 0.1 -1.1 N.D. -0.5 -0.5 -0.1
carbon source ferulate (C); with MOPS +0.1 N.D. +0.1 -2.3 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.6 N.D. -0.3 -1.2 +0.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 N.D. +0.2 -1.8 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.2 N.D. +0.1 -1.6 -0.2
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 N.D. -0.4 -1.6 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 N.D. -0.1 -1.4 -0.3
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.0 N.D. +0.1 -2.2 +0.3
nitrogen source Hippuric (N) +0.1 N.D. -0.1 -1.8 +0.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.1 N.D. +0.1 -1.9 -0.1
carbon source Delta-Decalactone (C) -0.1 N.D. -0.1 -1.6 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.3 N.D. -0.4 -1.4 -0.0
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.2 N.D. -0.0 -1.9 +0.2
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. -0.1 N.D. -0.2 -1.5 +0.3
nitrogen source D-2-Aminobutyric (N) +0.1 N.D. -0.4 -1.4 +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 N.D. +0.2 -1.6 +0.1
nitrogen source 2,6-diaminopimelic (N) -0.1 N.D. +0.3 -1.5 -0.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 N.D. +0.2 -1.7 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.5 N.D. +0.3 -1.5 +0.4
nitrogen source 2,6-diaminopimelic (N) +0.5 N.D. +0.4 -1.5 +0.1
pH Growth at pH9 and (C) Trisodium citrate +0.7 N.D. +0.2 -1.0 -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 N.D. +0.3 -0.9 -0.1
carbon source ferulate (C); with MOPS +0.7 N.D. +0.3 -0.7 +0.2
carbon source carbon source D-Glucose 55.5 mM +0.4 N.D. +1.0 +0.4 -0.2
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