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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_0970 and PP_0971 are separated by 109 nucleotides
PP_0971 and PP_0972 are separated by 16 nucleotides
PP_0972 and PP_0973 overlap by 53 nucleotides
PP_0973 and PP_0975 are separated by 369 nucleotides
PP_0970: PP_0970 - Cyanate MFS transporter, at 1,109,975 to 1,111,279
_0970
PP_0971: PP_0971 - conserved protein of unknown function, at 1,111,389 to 1,111,865
_0971
PP_0972: PP_0972 - UPF0213 protein PP_0972, at 1,111,882 to 1,112,199
_0972
PP_0973: PP_0973 - Nucleoid-associated protein PP_0973, at 1,112,147 to 1,113,154
_0973
PP_0975: PP_0975 - putative DNA-binding protein HU, form N, at 1,113,524 to 1,113,805
_0975
Group
Condition
PP
_0970
PP
_0971
PP
_0972
PP
_0973
PP
_0975
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.2
-0.5
-1.8
+0.1
N.D.
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
+0.1
-0.4
-1.3
-0.6
N.D.
phage
P. putida Sci small MOI 0.1
+0.3
-0.1
-2.6
+0.5
N.D.
phage
P. putida South MOI 10
-0.2
+0.1
-0.4
-1.0
N.D.
phage
P. putida Missley MOI 0.1
-0.4
-0.7
+0.4
-0.6
N.D.
nitrogen source
Urea (N); with MOPS
+0.0
+0.3
-1.0
-0.3
N.D.
stress
Growth with Chloride 500 mM; with MOPS
+0.1
+0.0
-1.2
+0.4
N.D.
carbon source
Myristic (C)
-0.1
+0.6
-1.0
-0.0
N.D.
reactor
72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
+0.1
-0.1
-1.2
+0.7
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.2
+0.3
-0.9
+0.2
N.D.
stress
MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM
-0.7
+0.5
+0.3
-0.6
N.D.
nitrogen source
2,6-diaminopimelic (N)
+0.5
+0.4
-1.3
+0.1
N.D.
pH
pH 5
+0.2
-0.8
-0.3
+0.6
N.D.
carbon source
Tween 20 (C)
+0.3
-0.2
-0.9
+0.7
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.3
+0.4
-1.1
+0.3
N.D.
pH
pH 7
+0.1
-0.2
-0.6
+0.9
N.D.
pH
pH 9
-0.0
-0.8
+0.1
+0.9
N.D.
pH
pH 6
+0.2
-0.5
-0.7
+1.3
N.D.
carbon source
carbon source 2-Deoxy-D-glucose 50 mM
+0.1
-0.1
-0.6
+0.9
N.D.
carbon source
carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM
+0.1
-0.4
+0.6
+0.7
N.D.
temperature
Growth at 30C; with MOPS
+0.1
-0.3
+0.8
+0.5
N.D.
nitrogen source
DL-2-Aminobutyric (N)
-0.3
+0.1
+0.4
+1.0
N.D.
nitrogen source
DL-2-Aminobutyric (N)
-0.3
+0.4
+0.6
+0.7
N.D.
stress
Growth with Chloride 500 mM; with MOPS
+0.4
-0.3
+0.9
+0.4
N.D.
nitrogen source
2,6-diaminopimelic (N)
-0.1
+0.6
+0.5
+0.6
N.D.
pH
Growth at pH6 and (C) Trisodium citrate
+0.1
+0.5
+0.5
+0.6
N.D.
stress
Growth with Chloride 500 mM; with MOPS
+0.2
+0.6
+0.6
+0.3
N.D.
nitrogen source
Propandiamine (N)
+0.4
+0.6
+0.4
+0.6
N.D.
carbon source
Trisodium citrate (C)
+0.2
+0.6
+0.8
+0.8
N.D.
nitrogen source
D-2-Aminobutyric (N)
-0.2
+1.0
+1.2
+2.0
N.D.
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