Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0943 and PP_0944 overlap by 11 nucleotidesPP_0944 and PP_0945 are separated by 27 nucleotidesPP_0945 and PP_0946 overlap by 20 nucleotidesPP_0946 and PP_0947 overlap by 4 nucleotides PP_0943: PP_0943 - conserved protein of unknown function, at 1,088,758 to 1,089,150 _0943 PP_0944: PP_0944 - fumarate hydratase (class 2), at 1,089,140 to 1,090,519 _0944 PP_0945: PP_0945 - conserved protein of unknown function, at 1,090,547 to 1,091,008 _0945 PP_0946: PP_0946 - superoxide dismutase (Mn), at 1,090,989 to 1,091,621 _0946 PP_0947: PP_0947 - conserved membrane protein of unknown function, at 1,091,618 to 1,092,526 _0947
Group Condition PP_0943 PP_0944 PP_0945 PP_0946 PP_0947
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 -0.3 -1.8 -0.2 -0.3
temperature Growth at 30C; with MOPS -0.5 -0.1 -2.5 +0.4 -0.1
carbon source ferulate (C); with MOPS -1.0 +0.1 -0.7 -0.4 -0.8
carbon source 3-methyl-1-butanol 0.1 vol% (C) -1.0 -0.4 -1.8 +0.3 +0.3
nitrogen source D-2-Aminobutyric (N) -1.2 +0.0 -0.6 -0.6 +0.2
temperature Growth at 25C -0.3 +0.2 -0.4 +0.1 -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -2.2 -0.3 +0.6 +0.2 +0.2
carbon source Nonanoic (C) -0.1 -0.3 -0.5 +0.3 -0.8
carbon source carbon source 3-O-methyl-glucose 10 mM -0.5 +0.5 -1.6 -0.2 +0.4
pH pH 4 +0.5 +0.3 -1.1 -0.7 -0.1
mixed carbon source mixed (C)s Trisodium citrate 10 mM and Parachlorophenylalanine 10 mM -0.9 -0.2 +0.5 -0.4 -0.1
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM -0.1 +0.4 -1.6 +0.3 -0.0
phage P. putida Sci Big MOI 0.1 +0.3 -0.8 -0.8 -0.1 +0.2
carbon source L-Leucine (C) -0.1 +0.0 -1.2 +0.6 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.7 -0.2 +0.6 -0.6 +0.1
temperature shift Temperature shift 30_to_21; with MOPS +0.4 +0.4 -0.5 -0.5 -0.4
nitrogen source NAG (N); with MOPS -0.5 -0.9 +0.5 +0.3 -0.1
carbon source D-Glucose (C) +0.5 -0.2 -1.4 +0.4 +0.4
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.4 -0.8 -0.4 +0.3 +0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.6 -0.5 +0.3 +0.3 +0.5
carbon source carbon source D-Glucose 55.5 mM +0.6 +0.6 -0.3 -0.8 +0.0
nitrogen source nitrogen source Ammonium chloride 20mM -0.1 -0.3 +1.2 -0.3 -0.3
carbon source D-Glucose (C); with MOPS +0.7 -0.4 -0.6 +0.4 +0.3
stress Growth with Chloride 500 mM; with MOPS -0.3 +0.4 +0.7 +0.2 -0.5
phage P. put South MOI 10 -0.1 -0.5 +1.0 +0.2 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 +0.1 +1.5 +0.1 -0.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.2 -0.6 +0.8 +0.6 +0.2
nitrogen source nitrogen source nitrite 0.5mM +0.3 +0.1 +1.1 +0.2 -0.4
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +1.1 -0.1 -0.1 +0.8 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.1 +0.1 +0.9 +0.4 +0.6
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