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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_0941 and PP_0942 are separated by 97 nucleotides
PP_0942 and PP_0943 are separated by 253 nucleotides
PP_0943 and PP_0944 overlap by 11 nucleotides
PP_0944 and PP_0945 are separated by 27 nucleotides
PP_0941: PP_0941 - putative ribosome associated protein, at 1,086,527 to 1,087,048
_0941
PP_0942: PP_0942 - regulatory protease, at 1,087,146 to 1,088,504
_0942
PP_0943: PP_0943 - conserved protein of unknown function, at 1,088,758 to 1,089,150
_0943
PP_0944: PP_0944 - fumarate hydratase (class 2), at 1,089,140 to 1,090,519
_0944
PP_0945: PP_0945 - conserved protein of unknown function, at 1,090,547 to 1,091,008
_0945
Group
Condition
PP
_0941
PP
_0942
PP
_0943
PP
_0944
PP
_0945
carbon source
3-methyl-1-butanol 0.1 vol% (C)
+0.2
-0.3
-1.0
-0.4
-1.8
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-1.4
-0.3
-0.2
-0.6
-0.7
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.5
+0.4
-0.7
-0.3
-1.8
temperature
Growth at 30C; with MOPS
+0.1
+0.2
-0.5
-0.1
-2.5
phage
P. putida PUT10 MOI 10
-1.9
-0.1
-0.1
-0.1
-0.3
nitrogen source
Urea (N)
-0.3
-0.3
-0.4
-0.1
-1.3
phage
PUT8 MOI 0.1
-1.4
+0.1
-0.4
-0.1
-0.3
carbon source
carbon source 3-O-methyl-glucose 10 mM
-0.2
-0.4
-0.5
+0.5
-1.6
phage
P. put South MOI 1
-1.7
-0.0
+0.2
-0.1
-0.6
carbon source
Ethyl decanoate (C)
-0.8
-0.9
+0.4
+0.1
-0.8
phage
P. putida Missley MOI 0.1
-1.4
+0.1
-0.3
-0.6
+0.1
phage
P. putida PUT10 MOI 10
-2.0
-0.3
+0.1
-0.1
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.2
-0.1
-2.2
-0.3
+0.6
phage
P. putida Sci Big MOI 0.1
+0.1
-0.4
+0.3
-0.8
-0.8
phage
P. putida South MOI 0.1
-1.9
-0.3
+0.1
+0.1
+0.6
phage
PUT 8 MOI 1
-1.6
+0.1
+0.3
+0.3
-0.5
carbon source
ferulate (C); with MOPS
+0.4
-0.1
-1.0
+0.1
-0.7
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM
+0.2
+0.1
-0.1
+0.4
-1.6
carbon source
L-Leucine (C)
-0.3
+0.7
-0.1
+0.0
-1.2
phage
P. putida South MOI 10
-1.2
-0.0
+0.4
-0.3
+0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.9
+0.3
-0.3
-0.4
+0.7
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
-0.6
+0.4
+0.0
+0.6
-0.8
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.5
+0.1
-0.4
+0.1
+1.5
nitrogen source
nitrogen source Ammonium chloride 20mM
+0.0
-0.8
-0.1
-0.3
+1.2
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.8
+0.4
+0.4
-0.8
-0.4
nitrogen source
nitrogen source nitrite 0.5mM
-0.2
-0.8
+0.3
+0.1
+1.1
nitrogen source
nitrogen source nitrate 20mM
+0.5
-0.3
-0.7
+0.3
+0.7
nitrogen source
D-2-Aminobutyric (N)
+0.3
+0.3
+0.3
+0.5
-0.9
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.8
+0.6
+0.2
-0.6
+0.8
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.9
+0.7
+1.1
-0.1
-0.1
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