Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0941 and PP_0942 are separated by 97 nucleotidesPP_0942 and PP_0943 are separated by 253 nucleotidesPP_0943 and PP_0944 overlap by 11 nucleotidesPP_0944 and PP_0945 are separated by 27 nucleotides PP_0941: PP_0941 - putative ribosome associated protein, at 1,086,527 to 1,087,048 _0941 PP_0942: PP_0942 - regulatory protease, at 1,087,146 to 1,088,504 _0942 PP_0943: PP_0943 - conserved protein of unknown function, at 1,088,758 to 1,089,150 _0943 PP_0944: PP_0944 - fumarate hydratase (class 2), at 1,089,140 to 1,090,519 _0944 PP_0945: PP_0945 - conserved protein of unknown function, at 1,090,547 to 1,091,008 _0945
Group Condition PP_0941 PP_0942 PP_0943 PP_0944 PP_0945
carbon source 3-methyl-1-butanol 0.1 vol% (C) +0.2 -0.3 -1.0 -0.4 -1.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.4 -0.3 -0.2 -0.6 -0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 +0.4 -0.7 -0.3 -1.8
temperature Growth at 30C; with MOPS +0.1 +0.2 -0.5 -0.1 -2.5
phage P. putida PUT10 MOI 10 -1.9 -0.1 -0.1 -0.1 -0.3
nitrogen source Urea (N) -0.3 -0.3 -0.4 -0.1 -1.3
phage PUT8 MOI 0.1 -1.4 +0.1 -0.4 -0.1 -0.3
carbon source carbon source 3-O-methyl-glucose 10 mM -0.2 -0.4 -0.5 +0.5 -1.6
phage P. put South MOI 1 -1.7 -0.0 +0.2 -0.1 -0.6
carbon source Ethyl decanoate (C) -0.8 -0.9 +0.4 +0.1 -0.8
phage P. putida Missley MOI 0.1 -1.4 +0.1 -0.3 -0.6 +0.1
phage P. putida PUT10 MOI 10 -2.0 -0.3 +0.1 -0.1 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.2 -0.1 -2.2 -0.3 +0.6
phage P. putida Sci Big MOI 0.1 +0.1 -0.4 +0.3 -0.8 -0.8
phage P. putida South MOI 0.1 -1.9 -0.3 +0.1 +0.1 +0.6
phage PUT 8 MOI 1 -1.6 +0.1 +0.3 +0.3 -0.5
carbon source ferulate (C); with MOPS +0.4 -0.1 -1.0 +0.1 -0.7
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM +0.2 +0.1 -0.1 +0.4 -1.6
carbon source L-Leucine (C) -0.3 +0.7 -0.1 +0.0 -1.2
phage P. putida South MOI 10 -1.2 -0.0 +0.4 -0.3 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.9 +0.3 -0.3 -0.4 +0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.6 +0.4 +0.0 +0.6 -0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.5 +0.1 -0.4 +0.1 +1.5
nitrogen source nitrogen source Ammonium chloride 20mM +0.0 -0.8 -0.1 -0.3 +1.2
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.8 +0.4 +0.4 -0.8 -0.4
nitrogen source nitrogen source nitrite 0.5mM -0.2 -0.8 +0.3 +0.1 +1.1
nitrogen source nitrogen source nitrate 20mM +0.5 -0.3 -0.7 +0.3 +0.7
nitrogen source D-2-Aminobutyric (N) +0.3 +0.3 +0.3 +0.5 -0.9
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.8 +0.6 +0.2 -0.6 +0.8
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.9 +0.7 +1.1 -0.1 -0.1
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