Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0928 and PP_0929 are separated by 60 nucleotidesPP_0929 and PP_0930 are separated by 126 nucleotidesPP_0930 and PP_0931 are separated by 11 nucleotidesPP_0931 and PP_0932 are separated by 15 nucleotides PP_0928: PP_0928 - K+-dependent Na+/Ca+ exchanger related-protein, at 1,070,342 to 1,071,385 _0928 PP_0929: PP_0929 - putative Lipoprotein, at 1,071,446 to 1,071,820 _0929 PP_0930: PP_0930 - Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B, at 1,071,947 to 1,073,392 _0930 PP_0931: PP_0931 - Glutamyl-tRNA(Gln) amidotransferase subunit A, at 1,073,404 to 1,074,855 _0931 PP_0932: PP_0932 - Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C, at 1,074,871 to 1,075,179 _0932
Group Condition PP_0928 PP_0929 PP_0930 PP_0931 PP_0932
phage P. putida South MOI 0.1 -0.8 -0.4 N.D. N.D. N.D.
phage PUT 8 MOI 1 -1.1 -0.0 N.D. N.D. N.D.
phage PUT8 MOI 0.1 -0.9 -0.1 N.D. N.D. N.D.
phage P. put South MOI 1 -0.7 -0.3 N.D. N.D. N.D.
phage PUT8 MOI 0.1 -1.1 +0.1 N.D. N.D. N.D.
nitrogen source NAG (N); with MOPS -0.3 -0.6 N.D. N.D. N.D.
phage P. putida South MOI 1 -0.9 -0.0 N.D. N.D. N.D.
phage P. putida South MOI 0.1 -1.0 +0.0 N.D. N.D. N.D.
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.1 -0.8 N.D. N.D. N.D.
phage P. put PUT 10 MOI 0.1 -0.8 -0.1 N.D. N.D. N.D.
phage P. putida PUT10 MOI 0.1 -0.8 -0.1 N.D. N.D. N.D.
pH Growth at pH7 and (C) D-Glucose -0.2 -0.7 N.D. N.D. N.D.
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.1 -0.7 N.D. N.D. N.D.
phage P. put PUT 10 MOI 1 -0.7 -0.2 N.D. N.D. N.D.
phage P. put PUT 10 MOI 10 -0.8 +0.1 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.2 -0.7 N.D. N.D. N.D.
phage P. put South MOI 0.1 -0.7 +0.2 N.D. N.D. N.D.
phage P. put South MOI 0.1 -0.7 +0.3 N.D. N.D. N.D.
phage P. putida PUT10 MOI 1 -0.7 +0.4 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 -0.6 N.D. N.D. N.D.
phage P. put PUT 10 MOI 0.1 -0.5 +0.3 N.D. N.D. N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.5 +0.4 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 +0.5 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.7 +0.8 N.D. N.D. N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.2 +0.7 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 +0.8 N.D. N.D. N.D.
stress R2A with Fusaric 480 ug/mL +0.6 +0.2 N.D. N.D. N.D.
phage P. putida Sci small MOI 0.1 +0.3 +0.6 N.D. N.D. N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs +0.5 +0.5 N.D. N.D. N.D.
carbon source L-Leucine (C) +0.4 +0.7 N.D. N.D. N.D.
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