Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0910 and PP_0911 are separated by 109 nucleotidesPP_0911 and PP_0912 are separated by 13 nucleotidesPP_0912 and PP_0913 are separated by 36 nucleotidesPP_0913 and PP_0914 are separated by 158 nucleotides PP_0910: PP_0910 - conserved exported protein of unknown function, at 1,051,939 to 1,053,366 _0910 PP_0911: PP_0911 - conserved protein of unknown function, at 1,053,476 to 1,053,862 _0911 PP_0912: PP_0912 - conserved protein of unknown function, at 1,053,876 to 1,054,463 _0912 PP_0913: PP_0913 - conserved exported protein of unknown function, at 1,054,500 to 1,055,831 _0913 PP_0914: PP_0914 - GGDEF domain protein, at 1,055,990 to 1,058,071 _0914
Group Condition PP_0910 PP_0911 PP_0912 PP_0913 PP_0914
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -2.6 +0.2 -0.4 -2.3 -2.5
carbon source 3-methyl-1-butanol 0.1 vol% (C) -3.8 -0.2 +0.2 -3.0 -0.0
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -2.2 +0.1 -0.1 -1.8 -1.7
stress R2A with Fusaric 480 ug/mL -2.6 -0.2 -0.1 -2.3 -0.0
stress R2A with Fusaric 480 ug/mL -2.7 -0.3 +0.3 -2.2 -0.2
pH pH 4 -2.0 +0.4 -0.1 -1.9 -1.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -2.5 -0.1 -0.1 -1.7 -0.2
carbon source Acetate Carbon Source (5mM) +0.0 -0.1 -0.1 -0.7 -3.5
stress R2A with Fusaric 480 ug/mL -2.1 -0.0 +0.1 -2.0 -0.2
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. -2.3 -0.0 -0.1 -1.9 +0.4
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -1.6 -0.2 -0.5 -2.1 +0.3
carbon source 1,5-Pentanediol (C) -0.2 -0.0 -0.0 -0.2 -3.6
stress R2A with Fusaric 480 ug/mL -1.9 +0.2 +0.2 -2.4 -0.0
carbon source L-Phenylalanine 5 mM (C) -0.4 +0.1 -0.4 -0.7 -2.5
carbon source M9 minimal media_noCarbon with D-Glucose 10 mM; 15C -1.1 +0.0 -0.0 -1.0 -1.8
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. -2.1 +0.0 -0.0 -2.0 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.3 +0.1 +0.1 -0.6 -3.0
carbon source Tween 20 (C) -1.1 -0.3 +0.3 -0.0 -2.6
carbon source Tween 20 (C) +0.1 -0.1 +0.0 +0.0 -3.7
stress R2A with Fusaric 480 ug/mL -2.0 -0.0 +0.2 -1.6 -0.2
carbon source Vanillin (C) -1.5 +0.3 -0.1 -1.3 -1.0
carbon source carbon source Glycerol 108.6 mM -1.9 +0.1 +0.2 -1.2 -0.6
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -1.6 -0.1 +0.1 -2.1 +0.3
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -2.0 -0.1 -0.0 -1.6 +0.6
anaerobic m9 anaerobic M9 -2.0 +0.2 -0.1 -1.3 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.4 +0.4 -0.2 +0.3 -2.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.7 -0.2 -0.1 -1.4 +1.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.7 +0.4 -0.4 -1.6 +1.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.1 -0.2 +0.4 -0.1 +2.3
stress Geraniol 1 vol% -0.5 +0.1 +0.1 +0.1 +4.3
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