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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_0880 and PP_0881 are separated by 10 nucleotides
PP_0881 and PP_0882 are separated by 91 nucleotides
PP_0882 and PP_0883 are separated by 54 nucleotides
PP_0883 and PP_0884 are separated by 228 nucleotides
PP_0880: PP_0880 - dipeptide ABC transporter - putative membrane subunit, at 1,020,039 to 1,020,965
_0880
PP_0881: PP_0881 - dipeptide ABC transporter - putative membrane subunit, at 1,020,976 to 1,021,986
_0881
PP_0882: PP_0882 - dipeptide ABC transporter - periplasmic binding protein, at 1,022,078 to 1,023,712
_0882
PP_0883: PP_0883 - glycine-glutamate dipeptide porin, at 1,023,767 to 1,025,146
_0883
PP_0884: PP_0884 - dipeptide ABC transporter - periplasmic binding protein, at 1,025,375 to 1,026,967
_0884
Group
Condition
PP
_0880
PP
_0881
PP
_0882
PP
_0883
PP
_0884
carbon source
L-Isoleucine (C)
-2.1
-2.5
-0.2
-0.3
-0.1
carbon source
L-Isoleucine (C)
-2.1
-2.1
-0.1
-0.2
+0.0
nitrogen source
D-2-Aminobutyric (N)
-1.8
-1.6
-0.6
-0.0
-0.4
carbon source
L-Leucine (C)
-1.9
-1.8
+0.0
-0.1
-0.0
carbon source
Ferulic Acid (C)
-2.4
-0.7
-0.3
+0.0
-0.1
carbon source
L-Lysine 10 mM (C)
-1.7
-1.3
+0.2
-0.3
-0.0
reactor
48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
-1.4
-1.1
-0.2
-0.0
-0.0
carbon source
L-Alanine (C)
-1.4
-1.9
+0.2
+0.0
+0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.6
-0.7
-0.8
-0.4
-0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.4
-1.8
-0.1
-0.0
-0.3
carbon source
3-methyl-1-butanol (C)
-1.2
-1.1
-0.1
+0.1
-0.1
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM
-1.8
-0.6
-0.0
+0.0
+0.0
reactor
72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
-1.4
-0.9
+0.1
-0.1
-0.0
carbon source
L-Leucine (C)
-0.7
-1.1
-0.6
+0.1
+0.1
nitrogen source
D-2-Aminobutyric (N)
-1.4
-0.5
+0.1
+0.3
-0.4
carbon source
D-Glucose (C)
-2.3
+0.1
+0.3
+0.1
-0.2
stress
MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM
-1.4
-0.6
+0.2
+0.2
-0.2
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
-1.0
-0.5
-0.4
-0.2
pH
Growth at pH7 and (C) D-Glucose
-2.1
+0.7
+0.4
-0.3
+0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
-0.5
+0.2
-0.6
-0.5
pH
pH 9
-1.1
-1.1
+0.6
+0.3
+0.5
pH
pH 4
+0.1
-1.7
+0.3
+0.3
+0.4
carbon source
carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM
-0.7
-0.7
+0.6
+0.3
+0.1
carbon source
Hexanoic (C)
+1.4
+1.1
-0.1
+0.3
+0.1
micoeukaryotes
C.elegans, mixed culture-2
+0.8
+0.8
+0.7
+0.2
+0.4
carbon source
Delta-Nonalactone (C)
+1.5
+1.2
+0.2
+0.2
-0.1
micoeukaryotes
Dictyostellum sp.mixed culture-2
+0.6
+0.8
+0.7
+0.3
+0.6
micoeukaryotes
C.elegans, mixed culture-1
+1.1
+0.9
+0.7
+0.4
+0.4
carbon source
3-methyl-1-butanol 0.25 vol% (C)
+2.1
+1.7
+0.3
-0.1
-0.1
carbon source
3-methyl-1-butanol 0.1 vol% (C)
+1.4
+1.3
+0.5
+0.2
+0.7
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