Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0873 and PP_0874 are separated by 52 nucleotidesPP_0874 and PP_0875 are separated by 33 nucleotidesPP_0875 and PP_0876 are separated by 40 nucleotidesPP_0876 and PP_5461 are separated by 276 nucleotides PP_0873: PP_0873 - putrescine-binding periplasmic protein, at 1,013,303 to 1,014,382 _0873 PP_0874: PP_0874 - conserved protein of unknown function, at 1,014,435 to 1,014,776 _0874 PP_0875: PP_0875 - conserved protein of unknown function, at 1,014,810 to 1,016,240 _0875 PP_0876: PP_0876 - Transcriptional regulator, AraC family, at 1,016,281 to 1,017,105 _0876 PP_5461: PP_5461 - protein of unknown function, at 1,017,382 to 1,017,861 _5461
Group Condition PP_0873 PP_0874 PP_0875 PP_0876 PP_5461
carbon source 1,3-Butandiol (C) +0.1 -6.6 +0.0 -0.4 +0.0
pH Growth at pH7 and (C) Trisodium citrate -0.2 -6.4 +0.1 -0.0 +0.4
carbon source L-Leucine (C) -0.0 -4.3 +0.2 -0.3 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.0 -4.4 -0.2 -0.0 +0.2
pH Growth at pH7 and (C) Trisodium citrate -0.3 -2.6 -0.3 -0.5 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.1 -3.5 -0.2 -0.1 +0.0
phage P. putida PUT 7 MOI 1 -0.0 -2.8 +0.0 -0.0 +0.2
temperature Growth at 30C; with MOPS -0.2 -1.9 -0.4 -0.0 -0.0
carbon source D-Glucose (C) -0.2 -1.0 -0.5 -0.7 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.0 -2.5 -0.0 +0.0 +0.2
carbon source carbon source D-Glucose 55.5 mM +0.1 -1.9 -0.2 -0.2 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.1 -1.5 -0.1 -0.4 -0.1
temperature Growth at 25C -0.3 -1.1 -0.0 -0.5 -0.1
carbon source D-Glucose (C); with MOPS -0.1 -1.8 +0.4 -0.2 -0.0
nitrogen source D-2-Aminobutyric (N) -0.2 -1.1 -0.3 +0.1 -0.3
nitrogen source nitrogen source nitrite 0.5mM -0.3 -2.3 +0.6 +0.2 +0.1
temperature shift Temperature shift 10_to_30; with MOPS +0.1 -1.7 +0.1 -0.2 -0.0
temperature shift Temperature shift 30_to_25; with MOPS +0.3 -1.4 -0.2 -0.3 +0.2
carbon source 4-Methyl-2-oxopentanoic (C) +0.1 -1.7 +0.1 +0.1 -0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 -1.9 +0.4 -0.1 -0.0
nitrogen source 2,6-diaminopimelic (N) +0.0 -1.8 +0.0 +0.2 +0.2
temperature shift Temperature shift 10_to_30 +0.2 -1.2 +0.2 -0.3 -0.1
carbon source ferulate (C); with MOPS +0.1 -1.8 +0.4 +0.2 -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.1 -1.6 +0.2 +0.3 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.6 +1.1 +0.1 -0.6 -0.0
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.1 +1.3 -0.4 -0.7 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +1.5 -0.3 -0.1 -0.1
r2a control with 0.2x vogels R2A control with 0.2X Vogels +0.2 +1.6 +0.2 -0.3 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.1 +1.2 -0.2 +0.6 +0.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +2.7 +0.5 +0.2 -0.1
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