Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0866 and PP_0867 are separated by 108 nucleotidesPP_0867 and PP_0868 are separated by 89 nucleotidesPP_0868 and PP_0869 overlap by 4 nucleotidesPP_0869 and PP_0870 overlap by 4 nucleotides PP_0866: PP_0866 - putative Transmembrane sensor, at 1,003,976 to 1,004,962 _0866 PP_0867: PP_0867 - FecA-like outer membrane receptor, at 1,005,071 to 1,007,500 _0867 PP_0868: PP_0868 - ABC transporter ATP-binding subunit, at 1,007,590 to 1,008,747 _0868 PP_0869: PP_0869 - osmoprotectant ABC transporter permease subunit, at 1,008,744 to 1,009,397 _0869 PP_0870: PP_0870 - putative Glycine betaine/carnitine/choline ABC transporter, periplasmic binding protein, at 1,009,394 to 1,010,335 _0870
Group Condition PP_0866 PP_0867 PP_0868 PP_0869 PP_0870
temperature Growth at 30C; with MOPS -0.0 +0.2 -0.5 -0.6 -1.0
carbon source D-Glucose (C) -0.1 +0.0 -0.6 -1.0 +0.2
stress R2A with Polymyxin B sulfate 0.001 mg/ml -0.2 +0.0 -0.2 -0.8 -0.2
pH Growth at pH7 and (C) Trisodium citrate +0.2 -0.1 -1.1 +0.3 -0.3
carbon source L-Leucine (C) -0.4 +0.0 +0.5 -0.6 -0.3
nitrogen source D-2-Aminobutyric (N) -0.2 +0.3 -0.8 -0.2 +0.4
nitrogen source 2,6-diaminopimelic (N) -0.5 -0.3 +0.7 +0.2 -0.5
carbon source carbon source 3-O-methyl-glucose 50 mM +0.2 +0.0 -0.2 -0.8 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.3 +0.2 +0.3 -0.5 -0.7
temperature shift Temperature shift 10_to_25; with MOPS +0.2 -0.1 -0.9 +0.3 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 +0.4 +0.4 -1.4 +0.0
stress Growth with Chloride 500 mM; with MOPS -0.5 +0.1 +0.4 -0.5 +0.2
temperature shift Temperature shift 30_to_10; with MOPS -0.2 +0.1 +0.5 -0.9 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.4 +0.1 +0.4 -0.6 -0.5
phage P. putida Sci Big MOI 0.1 -0.2 -0.0 +0.4 +0.5 -0.8
pH Growth at pH6 and (C) D-Glucose -0.2 +0.1 -0.5 +0.3 +0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 +0.4 +0.8 -0.9 +0.2
micoeukaryotes Tetrahymena sp., Mixed culture-2 -0.0 +0.4 +0.2 -0.5 +0.4
carbon source carbon source 4-vinylphenol 0.1 mM +0.4 -0.1 +0.6 -0.5 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.2 -0.2 +0.6 -0.2
carbon source carbon source 2-Deoxy-D-glucose 100 mM +0.2 -0.1 +0.5 -0.2 +0.5
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.5 -0.2 -0.2 +0.4 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.2 -0.0 +0.8 +0.8 -0.3
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.3 -0.2 +0.4 +0.3 +0.3
pH Growth at pH9 and (C) D-Glucose -0.2 +0.4 +0.2 +0.4 +0.4
pH pH 8 +0.4 -0.1 +0.3 +0.0 +0.7
carbon source 2-Methylbutanoic (C) -0.1 +0.0 +0.6 +0.4 +0.3
supernatant control:fungal media R2A with Vogels_fungal_media 0.2X +0.2 +0.1 +0.5 +0.4 +0.3
nitrogen source D-2-Aminobutyric (N) +0.1 +0.3 +0.2 +0.6 +0.5
temperature shift Temperature shift 30_to_21; with MOPS +0.1 +0.2 +0.6 +0.4 +0.8
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