Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0837 and PP_0838 are separated by 168 nucleotidesPP_0838 and PP_0839 are separated by 151 nucleotidesPP_0839 and PP_0840 are separated by 3 nucleotidesPP_0840 and PP_0841 are separated by 179 nucleotides PP_0837: PP_0837 - putative regulator of flagellation, at 974,609 to 975,151 _0837 PP_0838: PP_0838 - inositol monophosphatase / glycerol-2-phosphatase, at 975,320 to 976,138 _0838 PP_0839: PP_0839 - tRNA Cm32/Um32 methyltransferase, at 976,290 to 977,045 _0839 PP_0840: PP_0840 - serine acetyltransferase, at 977,049 to 977,834 _0840 PP_0841: PP_0841 - DNA-binding transcriptional dual regulator 2Fe-2S cluster IscR, at 978,014 to 978,505 _0841
Group Condition PP_0837 PP_0838 PP_0839 PP_0840 PP_0841
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 N.D. -0.1 -1.1 -1.2
pH Growth at pH6 and (C) D-Glucose +0.2 N.D. -0.7 -0.6 -1.5
stress R2A with Fusaric 240 ug/mL -0.4 N.D. -0.6 +0.2 -1.9
pH Growth at pH6 and (C) Trisodium citrate +0.1 N.D. -2.1 +0.3 -0.9
temperature shift Temperature shift 10_to_30 -0.3 N.D. -1.0 -0.2 -0.7
carbon source carbon source 4-vinylphenol 0.1 mM +0.0 N.D. -0.8 +0.6 -1.6
nitrogen source nitrate (N) -0.5 N.D. -1.3 -0.5 +0.5
phage P. putida PUT10 MOI 10 +0.4 N.D. -1.4 +0.5 -1.1
temperature shift Temperature shift 30_to_25; with MOPS +0.2 N.D. +0.3 -1.0 -1.0
phage P. putida PUT10 MOI 10 +0.3 N.D. -1.1 +0.3 -0.8
carbon source carbon source 2-Deoxy-D-glucose 50 mM -0.1 N.D. +0.6 -0.9 -0.9
phage P. put South MOI 1 +0.1 N.D. -1.0 +0.4 -0.8
carbon source carbon source D-Glucose 55.5 mM -0.1 N.D. +0.6 -0.5 -1.2
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. +0.2 N.D. -1.4 +0.4 -0.3
pH Growth at pH6 and (C) Trisodium citrate -0.3 N.D. +0.2 +0.5 -1.4
pH Growth at pH7 and (C) Trisodium citrate +0.1 N.D. -1.3 +0.5 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.6 N.D. -0.8 +0.4 -1.2
carbon source D-Glucose (C); with MOPS +0.1 N.D. -1.9 +0.5 +0.4
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM +0.2 N.D. +0.4 +0.3 -1.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.3 N.D. -0.2 -1.3 +1.0
carbon source butyrate (C) +0.2 N.D. -0.2 +0.5 -1.3
pH pH 8 -0.5 N.D. +0.4 +0.5 -1.0
pH Growth at pH7 and (C) Trisodium citrate +0.3 N.D. -1.4 +0.6 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 N.D. +0.0 +0.6 -1.2
phage PUT 8 MOI 1 +0.3 N.D. -1.2 +0.7 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 N.D. +0.2 +1.1 -1.1
micoeukaryotes Acanthamoeba sp., muxed culture-2 -0.1 N.D. -1.0 -0.2 +1.4
nitrogen source NAG (N); with MOPS +0.4 N.D. -1.2 +0.5 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.5 N.D. +1.0 -0.4 +0.4
temperature shift Temperature shift 30_to_21; with MOPS +0.6 N.D. +0.8 +0.9 +0.3
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