Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_t68 and PP_0832 are separated by 105 nucleotidesPP_0832 and PP_0833 are separated by 14 nucleotidesPP_0833 and PP_0834 are separated by 42 nucleotidesPP_0834 and PP_0835 are separated by 63 nucleotides PP_t68: PP_t68 - tRNA-Leu, at 968,898 to 968,982 _t68 PP_0832: PP_0832 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase, at 969,088 to 970,137 _0832 PP_0833: PP_0833 - queuine tRNA-ribosyltransferase, at 970,152 to 971,267 _0833 PP_0834: PP_0834 - Sec translocation complex subunit YajC, at 971,310 to 971,645 _0834 PP_0835: PP_0835 - Sec Translocation Complex SecD subunit, at 971,709 to 973,571 _0835
Group Condition PP_t68 PP_0832 PP_0833 PP_0834 PP_0835
phage P. put South MOI 10 N.D. +0.2 -3.7 -5.9 N.D.
micoeukaryotes Dictyostellum sp.mixed culture-2 N.D. -2.4 -4.0 -2.8 N.D.
phage P. putida South MOI 10 N.D. -0.1 -2.6 -6.0 N.D.
carbon source L-Lysine (C) N.D. -3.3 -1.9 -3.0 N.D.
pH Growth at pH6 and (C) Trisodium citrate N.D. -0.7 -1.1 -6.3 N.D.
carbon source Tween 20 (C) N.D. -0.2 -2.8 -4.9 N.D.
micoeukaryotes Tetrahymena sp., Mixed culture-2 N.D. -2.3 -3.6 -2.1 N.D.
nitrogen source spermidine (N) N.D. -1.5 -1.3 -4.7 N.D.
micoeukaryotes Dictyostellum sp.mixed culture-1 N.D. -3.2 -2.0 -2.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. -2.3 -2.0 -3.1 N.D.
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.0 -1.2 -5.2 N.D.
nitrogen source Propandiamine (N) N.D. -0.5 -1.1 -5.8 N.D.
micoeukaryotes Tetrahymena sp., Mixed culture-1 N.D. -1.7 -4.0 -1.4 N.D.
nitrogen source spermidine (N) N.D. -1.0 -2.8 -3.1 N.D.
nitrogen source Beta-alanine (N) N.D. -3.0 -4.0 +0.0 N.D.
micoeukaryotes C.elegans, mixed culture-1 N.D. -2.0 -3.2 -1.1 N.D.
micoeukaryotes Acanthamoeba sp., muxed culture-2 N.D. -0.3 -3.1 -2.9 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs N.D. -0.8 -0.6 -4.6 N.D.
carbon source 3-methyl-1-butanol 0.1 vol% (C) N.D. -3.1 -3.2 +0.3 N.D.
stress R2A with Fusaric 480 ug/mL N.D. -0.6 -4.1 -1.2 N.D.
carbon source D-Glucuronic gamma lactone (C) N.D. -0.6 -2.4 -2.6 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs N.D. -3.1 -1.5 -1.0 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs N.D. -2.1 -1.7 -1.8 N.D.
carbon source Levulinic Acid (C) N.D. -1.4 -4.3 +0.1 N.D.
carbon source Levulinic Acid (C) N.D. -1.6 -3.8 -0.1 N.D.
nitrogen source D-2-Aminobutyric (N) N.D. -1.4 -3.2 +1.7 N.D.
carbon source Lignin Monomers (C) N.D. +0.2 +1.1 -4.2 N.D.
carbon source L-Leucine (C) N.D. +1.1 +1.5 -4.8 N.D.
carbon source carbon source 4-vinylphenol 0.1 mM N.D. +1.0 +2.3 -2.4 N.D.
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay N.D. +1.6 +2.5 +1.6 N.D.
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