Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0828 and PP_0829 are separated by 318 nucleotidesPP_0829 and PP_0830 are separated by 10 nucleotidesPP_0830 and PP_0831 are separated by 167 nucleotidesPP_0831 and PP_t68 are separated by 330 nucleotides PP_0828: PP_0828 - conserved membrane protein of unknown function, at 965,823 to 966,716 _0828 PP_0829: PP_0829 - conserved protein of unknown function, at 967,035 to 967,331 _0829 PP_0830: PP_0830 - Acetyltransferase, GNAT family, at 967,342 to 967,821 _0830 PP_0831: PP_0831 - hypothetical protein, at 967,989 to 968,567 _0831 PP_t68: PP_t68 - tRNA-Leu, at 968,898 to 968,982 _t68
Group Condition PP_0828 PP_0829 PP_0830 PP_0831 PP_t68
nitrogen source Ammonium chloride (N) -1.2 +0.2 -0.5 -0.3 N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.8 +0.0 -0.6 -0.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 -0.1 +0.0 -0.9 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.3 -0.2 -0.2 -0.9 N.D.
temperature shift Temperature shift 10_to_30; with MOPS -0.8 -0.0 +0.3 -1.1 N.D.
attachment on pdms attachment on PDMS; 2 hours -0.1 -0.0 -0.9 -0.5 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -0.3 +0.2 -1.2 N.D.
nitrogen source nitrate (N) -0.2 -0.1 +0.0 -1.0 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +0.0 +0.1 -1.1 N.D.
temperature shift Temperature shift 30_to_25; with MOPS -0.4 +0.1 +0.1 -0.9 N.D.
nitrogen source Propandiamine (N) -0.5 -0.1 +0.1 -0.7 N.D.
biofilm mediium pdms biofilm mediium PDMS; 7 hours -0.1 -0.0 +0.2 -1.1 N.D.
carbon source D-Glucose (C) +0.3 -0.0 -0.4 -0.9 N.D.
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. -0.3 +0.2 +0.1 -0.8 N.D.
carbon source carbon source 3-O-methyl-glucose 50 mM -0.2 -0.3 +0.4 -0.6 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.6 -0.1 -0.2 -0.9 N.D.
carbon source carbon source Glycerol 108.6 mM -0.3 -0.5 +0.5 -0.1 N.D.
carbon source carbon source 2-Deoxy-D-glucose 50 mM -0.2 -0.1 +0.5 -0.6 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.5 +0.2 -0.2 -0.7 N.D.
stress R2A with Fusaric 120 ug/mL +0.2 -0.3 +0.4 -0.5 N.D.
pH pH 4 -0.2 -0.2 +0.8 -0.4 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.3 +0.2 +0.3 -0.6 N.D.
carbon source carbon source 3-O-methyl-glucose 10 mM -0.0 -0.1 +0.8 -0.5 N.D.
temperature shift Temperature shift 30_to_21; with MOPS +0.6 -0.1 +0.5 -0.7 N.D.
carbon source carbon source 2-Deoxy-D-glucose 100 mM -0.5 -0.2 +0.7 +0.3 N.D.
pH pH 9 -0.1 -0.3 +1.0 -0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +0.1 +0.5 -0.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 +0.4 +0.5 +0.2 N.D.
carbon source D-Glucose (C) +0.6 +0.2 +0.3 -0.4 N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.5 +0.3 +0.9 +0.3 N.D.
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