Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0797 and PP_0798 overlap by 4 nucleotidesPP_0798 and PP_0799 are separated by 198 nucleotidesPP_0799 and PP_5458 are separated by 205 nucleotidesPP_5458 and PP_0800 are separated by 190 nucleotides PP_0797: PP_0797 - conserved protein of unknown function, at 915,540 to 916,079 _0797 PP_0798: PP_0798 - GGDEF domain protein, at 916,076 to 917,566 _0798 PP_0799: PP_0799 - histidine-specific outer membrane porin D, at 917,765 to 919,099 _0799 PP_5458: PP_5458 - conserved exported protein of unknown function, at 919,305 to 919,529 _5458 PP_0800: PP_0800 - conserved protein of unknown function, at 919,720 to 920,472 _0800
Group Condition PP_0797 PP_0798 PP_0799 PP_5458 PP_0800
phage P. putida Sci Big MOI 0.1 -0.4 -0.5 -0.3 N.D. -0.3
carbon source ferulate (C); with MOPS -0.0 -0.2 -0.2 N.D. -1.1
phage P. putida Sci small MOI 0.1 +0.3 -1.1 -0.2 N.D. -0.1
carbon source ferulate (C); with MOPS -0.3 -0.4 +0.1 N.D. -0.3
temperature shift Temperature shift 10_to_25; with MOPS +0.1 -0.4 -0.1 N.D. -0.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.9 -0.3 +0.0 N.D. +0.3
nitrogen source 2,6-diaminopimelic (N) +0.1 -0.1 +0.1 N.D. -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.5 -0.4 -0.0 N.D. +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.6 +0.2 -0.2 N.D. +0.1
pH Growth at pH6 and (C) D-Glucose +0.3 -0.5 +0.1 N.D. -0.3
carbon source Crotonic (C) -0.6 +0.4 +0.1 N.D. +0.1
carbon source Tween 20 (C) +0.5 +0.0 +0.3 N.D. -0.7
carbon source L-Leucine (C) -0.8 +0.5 +0.0 N.D. +0.5
carbon source acetate (C) +0.6 +0.1 -0.2 N.D. -0.3
no stress control Growth with no stress +0.7 -0.1 -0.1 N.D. -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 +0.7 +0.2 N.D. -0.2
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.6 -0.4 +0.1 N.D. +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.8 -0.1 -0.2 N.D. +0.0
nitrogen source nitrogen source nitrate 5mM +0.7 -0.0 -0.1 N.D. +0.3
carbon source Valeric (C) +0.5 +0.2 -0.2 N.D. +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.5 -0.1 +0.1 N.D. +0.4
carbon source carbon source 3-O-methyl-glucose 10 mM +0.7 +0.2 +0.2 N.D. -0.1
pH pH 8 +0.6 +0.1 +0.2 N.D. +0.1
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 +0.0 -0.0 N.D. +0.9
pH pH 5 +0.4 +0.4 +0.0 N.D. +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.4 +0.2 +0.2 N.D. +0.4
pH pH 9 +0.4 +0.3 +0.2 N.D. +0.3
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 +0.0 +0.0 N.D. +1.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.9 +0.0 +0.2 N.D. +0.1
pH pH 7 +0.7 +0.2 +0.2 N.D. +0.4
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