Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0769 and PP_0770 are separated by 193 nucleotidesPP_0770 and PP_0771 overlap by 4 nucleotidesPP_0771 and PP_0772 are separated by 18 nucleotidesPP_0772 and PP_0773 are separated by 118 nucleotides PP_0769: PP_0769 - Sensor histidine kinase, at 888,571 to 889,263 _0769 PP_0770: PP_0770 - PemI-like protein, at 889,457 to 889,711 _0770 PP_0771: PP_0771 - mRNA interferase, at 889,708 to 890,055 _0771 PP_0772: PP_0772 - Metallo-beta-lactamase family protein, at 890,074 to 890,718 _0772 PP_0773: PP_0773 - OmpA/MotB domain-containing protein, at 890,837 to 891,547 _0773
Group Condition PP_0769 PP_0770 PP_0771 PP_0772 PP_0773
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 -2.0 +0.0 -0.3 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 -1.6 -0.2 +0.1 -0.5
carbon source Valeric (C) -0.5 -0.8 -0.3 -0.0 -0.6
pH pH 4 +0.1 -0.5 -0.5 -0.6 -0.7
carbon source D-Glucose (C) 10 mM -0.3 -0.4 -0.3 -0.3 -0.7
r2a control R2A control -0.0 -0.3 -0.4 -0.9 -0.4
phage P. put PUT 10 MOI 0.1 +0.3 -1.8 -0.6 +0.1 -0.1
carbon source L-Leucine (C) +0.0 -1.5 -0.7 -0.1 +0.3
carbon source Myristic (C) -0.6 -0.3 -0.6 +0.3 -0.3
nitrogen source D-2-Aminobutyric (N) +0.2 -0.8 -0.6 +0.2 -0.4
stress R2A with Fusaric 480 ug/mL +0.3 -0.9 +0.2 -0.3 -0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.8 -0.9 +0.4 +0.1 -0.0
pH Valerolactone (C) pH 6.5 -0.8 -0.4 -0.4 -0.1 +0.4
carbon source D-Glucose (C) +0.0 +0.5 +0.1 -0.9 -0.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -0.4 -1.3 +0.1 +0.2 +0.2
carbon source carbon source Bisabolol 45 mM +0.6 -0.4 -0.5 -0.5 -0.3
carbon source ferulate (C); with MOPS +0.2 +0.1 -0.4 -1.4 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.2 +0.1 +0.2 -1.1 -0.4
pH Growth at pH7 and (C) Trisodium citrate -0.6 -0.3 +0.6 +0.1 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.0 -1.2 +0.3 +0.4 -0.4
pH pH 5 +0.8 -0.6 -0.0 -0.8 -0.2
carbon source carbon source D-Glucose 55.5 mM +0.8 -0.4 -0.1 -0.7 -0.2
nitrogen source D-2-Aminobutyric (N) +0.5 -0.4 +0.3 -0.3 -0.7
pH pH 8 +0.5 +0.1 +0.1 -0.7 -0.6
nitrogen source Propiolactam (N) +0.4 -0.8 -0.6 +0.4 +0.0
phage P. putida South MOI 10 +0.2 -0.3 -0.8 +0.3 +0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.6 -1.3 +0.8 +0.3 -0.1
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. +0.5 +0.5 -0.1 -0.6 +0.4
carbon source D-Glucose (C); with MOPS -0.5 +0.7 +0.1 +0.8 +0.3
pH Growth at pH8 and (C) D-Glucose -0.1 +0.7 +0.1 +0.6 +0.5
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