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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_0744 and PP_0745 are separated by 35 nucleotides
PP_0745 and PP_0746 are separated by 2 nucleotides
PP_0746 and PP_0747 are separated by 162 nucleotides
PP_0747 and PP_0748 are separated by 2 nucleotides
PP_0744: PP_0744 - Ferrochelatase, at 862,867 to 863,883
_0744
PP_0745: PP_0745 - putative uracil permease, at 863,919 to 865,184
_0745
PP_0746: PP_0746 - Uracil phosphoribosyltransferase, at 865,187 to 865,825
_0746
PP_0747: PP_0747 - Hypoxanthine-guanine phosphoribosyltransferase, at 865,988 to 866,545
_0747
PP_0748: PP_0748 - conserved protein of unknown function, at 866,548 to 867,021
_0748
Group
Condition
PP
_0744
PP
_0745
PP
_0746
PP
_0747
PP
_0748
carbon source
3-methyl-1-butanol 0.1 vol% (C)
N.D.
-0.8
-1.9
-0.1
-0.1
carbon source
L-Phenylalanine 5 mM (C)
N.D.
-0.5
-0.4
-0.3
-0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+0.1
-1.0
+0.1
-0.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
N.D.
+0.2
+0.1
-1.1
-0.5
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
N.D.
-0.6
-0.6
+0.2
+0.0
carbon source
1,3-Butandiol (C)
N.D.
-0.2
-0.2
+0.3
-0.7
nitrogen source
Ammonium chloride (N)
N.D.
-0.5
+0.3
-0.5
-0.1
phage
P. putida Sci Big MOI 0.1
N.D.
-0.1
+0.3
-0.8
-0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
N.D.
+0.5
+0.0
-0.8
-0.2
carbon source
Valerolactone (C)
N.D.
-0.3
-0.3
-0.4
+0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days
N.D.
+0.3
-0.3
+0.5
-1.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
N.D.
+0.1
-1.0
+0.3
+0.1
r2a control with 0.2% methanol
R2A control with 0.2% methanol
N.D.
-0.0
+0.4
-0.3
-0.5
reactor
48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay
N.D.
-0.3
-0.4
+0.5
-0.1
carbon source
carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM
N.D.
-0.2
+0.4
+0.1
-0.7
nitrogen source
D-2-Aminobutyric (N)
N.D.
-0.2
-1.0
+0.3
+0.6
pH
pH 8
N.D.
-0.2
+0.2
+0.4
-0.7
carbon source
carbon source Bisabolene 48.9 mM
N.D.
-0.5
+0.7
+0.0
-0.4
pH
pH 5
N.D.
-0.6
+0.6
+0.2
-0.1
carbon source
carbon source 3-O-methyl-glucose 50 mM
N.D.
-0.6
+0.4
+0.3
-0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+0.3
+0.0
+0.5
-0.5
carbon source
carbon source 4-vinylphenol 0.01 mM
N.D.
-0.7
+0.9
+0.5
-0.3
carbon source
carbon source 3-O-methyl-glucose 10 mM
N.D.
-0.6
+0.5
+0.4
+0.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-0.2
-0.4
+0.5
+0.6
carbon source
D-Glucose (C)
N.D.
-0.1
+0.3
+0.7
-0.4
carbon source
carbon source Glycerol 108.6 mM
N.D.
-0.4
+0.5
+0.5
-0.0
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+0.7
-0.3
+0.2
+0.5
reactor
48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
N.D.
+0.9
+0.2
+0.2
+0.1
phage
P. putida South MOI 0.1
N.D.
+0.2
+0.3
+0.6
+0.3
nitrogen source
NAG (N); with MOPS
N.D.
+0.7
+0.3
+0.4
+0.1
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