Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0744 and PP_0745 are separated by 35 nucleotidesPP_0745 and PP_0746 are separated by 2 nucleotidesPP_0746 and PP_0747 are separated by 162 nucleotidesPP_0747 and PP_0748 are separated by 2 nucleotides PP_0744: PP_0744 - Ferrochelatase, at 862,867 to 863,883 _0744 PP_0745: PP_0745 - putative uracil permease, at 863,919 to 865,184 _0745 PP_0746: PP_0746 - Uracil phosphoribosyltransferase, at 865,187 to 865,825 _0746 PP_0747: PP_0747 - Hypoxanthine-guanine phosphoribosyltransferase, at 865,988 to 866,545 _0747 PP_0748: PP_0748 - conserved protein of unknown function, at 866,548 to 867,021 _0748
Group Condition PP_0744 PP_0745 PP_0746 PP_0747 PP_0748
carbon source 3-methyl-1-butanol 0.1 vol% (C) N.D. -0.8 -1.9 -0.1 -0.1
carbon source L-Phenylalanine 5 mM (C) N.D. -0.5 -0.4 -0.3 -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.1 -1.0 +0.1 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs N.D. +0.2 +0.1 -1.1 -0.5
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. -0.6 -0.6 +0.2 +0.0
carbon source 1,3-Butandiol (C) N.D. -0.2 -0.2 +0.3 -0.7
nitrogen source Ammonium chloride (N) N.D. -0.5 +0.3 -0.5 -0.1
phage P. putida Sci Big MOI 0.1 N.D. -0.1 +0.3 -0.8 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. +0.5 +0.0 -0.8 -0.2
carbon source Valerolactone (C) N.D. -0.3 -0.3 -0.4 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. +0.3 -0.3 +0.5 -1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.1 -1.0 +0.3 +0.1
r2a control with 0.2% methanol R2A control with 0.2% methanol N.D. -0.0 +0.4 -0.3 -0.5
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay N.D. -0.3 -0.4 +0.5 -0.1
carbon source carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM N.D. -0.2 +0.4 +0.1 -0.7
nitrogen source D-2-Aminobutyric (N) N.D. -0.2 -1.0 +0.3 +0.6
pH pH 8 N.D. -0.2 +0.2 +0.4 -0.7
carbon source carbon source Bisabolene 48.9 mM N.D. -0.5 +0.7 +0.0 -0.4
pH pH 5 N.D. -0.6 +0.6 +0.2 -0.1
carbon source carbon source 3-O-methyl-glucose 50 mM N.D. -0.6 +0.4 +0.3 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.3 +0.0 +0.5 -0.5
carbon source carbon source 4-vinylphenol 0.01 mM N.D. -0.7 +0.9 +0.5 -0.3
carbon source carbon source 3-O-methyl-glucose 10 mM N.D. -0.6 +0.5 +0.4 +0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.2 -0.4 +0.5 +0.6
carbon source D-Glucose (C) N.D. -0.1 +0.3 +0.7 -0.4
carbon source carbon source Glycerol 108.6 mM N.D. -0.4 +0.5 +0.5 -0.0
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.7 -0.3 +0.2 +0.5
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. N.D. +0.9 +0.2 +0.2 +0.1
phage P. putida South MOI 0.1 N.D. +0.2 +0.3 +0.6 +0.3
nitrogen source NAG (N); with MOPS N.D. +0.7 +0.3 +0.4 +0.1
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