Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0726 and PP_0727 overlap by 4 nucleotidesPP_0727 and PP_0728 overlap by 4 nucleotidesPP_0728 and PP_0729 are separated by 0 nucleotidesPP_0729 and PP_0730 are separated by 59 nucleotides PP_0726: PP_0726 - Transferase, at 843,518 to 844,243 _0726 PP_0727: PP_0727 - conserved protein of unknown function, at 844,240 to 844,842 _0727 PP_0728: PP_0728 - conserved protein of unknown function, at 844,839 to 846,008 _0728 PP_0729: PP_0729 - conserved protein of unknown function, at 846,009 to 847,199 _0729 PP_0730: PP_0730 - conserved protein of unknown function, at 847,259 to 848,569 _0730
Group Condition PP_0726 PP_0727 PP_0728 PP_0729 PP_0730
phage P. putida PUT 7 MOI 1 -0.5 -0.1 -0.4 -0.1 -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.0 -0.5 +0.1 -0.2 -0.0
nitrogen source nitrate (N) -0.3 -0.3 +0.1 -0.1 -0.1
pH Growth at pH6 and (C) D-Glucose +0.0 -0.3 +0.1 -0.0 -0.3
nitrogen source D-2-Aminobutyric (N) -0.1 -0.4 -0.0 +0.2 -0.1
pH Growth at pH6 and (C) Trisodium citrate -0.0 -0.3 -0.3 +0.1 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.3 -0.1 -0.2 -0.0
nitrogen source D-2-Aminobutyric (N) -0.1 -0.4 -0.5 +0.3 +0.3
temperature shift Temperature shift 30_to_25 -0.1 -0.2 +0.2 +0.1 -0.2
phage P. putida Sci Big MOI 0.1 +0.0 -0.2 -0.3 +0.1 +0.1
carbon source ferulate (C); with MOPS -0.3 +0.4 +0.0 -0.2 -0.1
temperature Growth at 30C +0.2 -0.3 +0.1 +0.0 -0.2
nitrogen source nitrate (N) +0.1 +0.3 -0.2 -0.1 -0.2
pH Valerolactone (C) pH 6.5 +0.1 -0.1 +0.0 +0.2 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.2 -0.2 +0.2 -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.3 +0.3 +0.1 -0.1 -0.0
carbon source carbon source 4-vinylphenol 0.01 mM +0.3 -0.1 +0.3 -0.1 -0.2
pH Growth at pH8 and (C) D-Glucose +0.1 -0.2 +0.3 +0.1 -0.1
carbon source D-Glucose (C); with MOPS +0.1 -0.3 +0.2 +0.1 +0.1
pH Growth at pH7 and (C) Trisodium citrate -0.3 -0.0 +0.1 +0.2 +0.2
carbon source L-Lysine (C) +0.1 +0.4 -0.2 -0.1 +0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.1 +0.3 +0.1 -0.1 +0.2
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2 +0.5 +0.3 +0.0 +0.1
phage P. putida Sci small MOI 0.1 +0.0 +0.5 +0.2 -0.1 +0.1
pH pH 8 +0.2 +0.3 +0.3 +0.0 -0.0
carbon source carbon source Glycerol 108.6 mM +0.2 +0.2 +0.3 +0.0 +0.0
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.2 +0.4 +0.2 +0.1 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 +0.4 +0.1 +0.1 -0.1
stress Growth with Chloride 500 mM; with MOPS +0.2 +0.2 +0.2 +0.1 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.5 +0.2 -0.0 +0.2 +0.2
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