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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_0699 and PP_t70 are separated by 130 nucleotides
PP_t70 and PP_0700 are separated by 95 nucleotides
PP_0700 and PP_0701 are separated by 130 nucleotides
PP_0701 and PP_0702 are separated by 324 nucleotides
PP_0699: PP_0699 - Transporter, LysE family, at 812,479 to 813,093
_0699
PP_t70: PP_t70 - tRNA-Met, at 813,224 to 813,300
_t70
PP_0700: PP_0700 - putative Transmembrane sensor, at 813,396 to 814,382
_0700
PP_0701: PP_0701 - fosmidomycin efflux system, at 814,513 to 815,727
_0701
PP_0702: PP_0702 - Major facilitator family transporter, at 816,052 to 817,209
_0702
Group
Condition
PP
_0699
PP
_t70
PP
_0700
PP
_0701
PP
_0702
pH
Growth at pH7 and (C) D-Glucose
-1.6
N.D.
+0.1
-0.2
-0.0
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.5
N.D.
-0.5
-0.1
-0.4
reactor
72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
-0.6
N.D.
-0.9
+0.0
+0.0
nitrogen source
Urea (N)
-0.3
N.D.
-0.3
-0.0
-0.6
nitrogen source
D-2-Aminobutyric (N)
-0.7
N.D.
-0.3
-0.3
+0.1
pH
Growth at pH8 and (C) D-Glucose
-0.7
N.D.
-0.0
-0.4
+0.1
carbon source
carbon source 3-O-methyl-glucose 50 mM
+0.1
N.D.
-0.3
-0.4
-0.4
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
-0.5
N.D.
-0.6
-0.0
+0.2
phage
P. putida Sci small MOI 0.1
-0.4
N.D.
+0.3
-0.2
-0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days
-0.1
N.D.
-0.6
-0.3
+0.2
phage
P. putida Sci Big MOI 0.1
+0.3
N.D.
-0.5
-0.3
-0.2
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.5
N.D.
-0.4
+0.2
+0.1
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.1
N.D.
-0.9
+0.1
+0.2
temperature shift
Temperature shift 10_to_30; with MOPS
-0.2
N.D.
+0.3
-0.6
-0.0
carbon source
carbon source 4-vinylphenol 0.1 mM
-0.3
N.D.
-0.3
+0.3
-0.2
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.0
N.D.
-0.9
+0.0
+0.4
nitrogen source
Urea (N); with MOPS
+0.3
N.D.
-0.4
+0.2
-0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.7
N.D.
-0.0
+0.4
+0.1
temperature shift
Temperature shift 30_to_25; with MOPS
+0.6
N.D.
-0.4
-0.3
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.1
N.D.
-0.4
-0.2
+0.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
-0.5
N.D.
+0.2
+0.1
+0.3
temperature shift
Temperature shift 30_to_10; with MOPS
+0.5
N.D.
-0.1
-0.4
+0.1
carbon source
3-methyl-1-butanol 0.1 vol% (C)
+0.2
N.D.
+0.2
-0.5
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.3
N.D.
+0.4
-0.3
-0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.3
N.D.
-0.1
+0.6
-0.4
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
N.D.
+0.4
-0.3
+0.2
pH
Growth at pH6 and (C) D-Glucose
+0.6
N.D.
+0.3
-0.2
+0.3
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.5
N.D.
+0.4
+0.2
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
+0.3
N.D.
-0.1
+0.3
+0.5
nitrogen source
2,6-diaminopimelic (N)
+1.1
N.D.
+0.3
-0.0
-0.2
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