Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0699 and PP_t70 are separated by 130 nucleotidesPP_t70 and PP_0700 are separated by 95 nucleotidesPP_0700 and PP_0701 are separated by 130 nucleotidesPP_0701 and PP_0702 are separated by 324 nucleotides PP_0699: PP_0699 - Transporter, LysE family, at 812,479 to 813,093 _0699 PP_t70: PP_t70 - tRNA-Met, at 813,224 to 813,300 _t70 PP_0700: PP_0700 - putative Transmembrane sensor, at 813,396 to 814,382 _0700 PP_0701: PP_0701 - fosmidomycin efflux system, at 814,513 to 815,727 _0701 PP_0702: PP_0702 - Major facilitator family transporter, at 816,052 to 817,209 _0702
Group Condition PP_0699 PP_t70 PP_0700 PP_0701 PP_0702
pH Growth at pH7 and (C) D-Glucose -1.6 N.D. +0.1 -0.2 -0.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.5 N.D. -0.5 -0.1 -0.4
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.6 N.D. -0.9 +0.0 +0.0
nitrogen source Urea (N) -0.3 N.D. -0.3 -0.0 -0.6
nitrogen source D-2-Aminobutyric (N) -0.7 N.D. -0.3 -0.3 +0.1
pH Growth at pH8 and (C) D-Glucose -0.7 N.D. -0.0 -0.4 +0.1
carbon source carbon source 3-O-methyl-glucose 50 mM +0.1 N.D. -0.3 -0.4 -0.4
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.5 N.D. -0.6 -0.0 +0.2
phage P. putida Sci small MOI 0.1 -0.4 N.D. +0.3 -0.2 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.1 N.D. -0.6 -0.3 +0.2
phage P. putida Sci Big MOI 0.1 +0.3 N.D. -0.5 -0.3 -0.2
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.5 N.D. -0.4 +0.2 +0.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.1 N.D. -0.9 +0.1 +0.2
temperature shift Temperature shift 10_to_30; with MOPS -0.2 N.D. +0.3 -0.6 -0.0
carbon source carbon source 4-vinylphenol 0.1 mM -0.3 N.D. -0.3 +0.3 -0.2
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.0 N.D. -0.9 +0.0 +0.4
nitrogen source Urea (N); with MOPS +0.3 N.D. -0.4 +0.2 -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.7 N.D. -0.0 +0.4 +0.1
temperature shift Temperature shift 30_to_25; with MOPS +0.6 N.D. -0.4 -0.3 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 N.D. -0.4 -0.2 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.5 N.D. +0.2 +0.1 +0.3
temperature shift Temperature shift 30_to_10; with MOPS +0.5 N.D. -0.1 -0.4 +0.1
carbon source 3-methyl-1-butanol 0.1 vol% (C) +0.2 N.D. +0.2 -0.5 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3 N.D. +0.4 -0.3 -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 N.D. -0.1 +0.6 -0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 N.D. +0.4 -0.3 +0.2
pH Growth at pH6 and (C) D-Glucose +0.6 N.D. +0.3 -0.2 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 N.D. +0.4 +0.2 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.3 N.D. -0.1 +0.3 +0.5
nitrogen source 2,6-diaminopimelic (N) +1.1 N.D. +0.3 -0.0 -0.2
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