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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_0651 and PP_5453 are separated by 833 nucleotides
PP_5453 and PP_0652 are separated by 64 nucleotides
PP_0652 and PP_0653 overlap by 4 nucleotides
PP_0653 and PP_0654 are separated by 151 nucleotides
PP_0651: PP_0651 - Acetyltransferase, GNAT family, at 758,579 to 759,028
_0651
PP_5453: PP_5453 - conserved protein of unknown function, at 759,862 to 760,089
_5453
PP_0652: PP_0652 - Gluconate transporter, at 760,154 to 761,539
_0652
PP_0653: PP_0653 - conserved protein of unknown function, at 761,536 to 761,817
_0653
PP_0654: PP_0654 - putative malate dehydrogenase, at 761,969 to 762,901
_0654
Group
Condition
PP
_0651
PP
_5453
PP
_0652
PP
_0653
PP
_0654
temperature
Growth at 25C
+0.0
-5.4
-0.2
-5.7
-0.2
carbon source
D-Lysine 10 mM (C)
-0.1
-0.9
+0.1
-5.6
-0.1
carbon source
ferulate (C); with MOPS
-0.0
-1.0
+0.1
-5.2
-0.3
nitrogen source
Hydantoin (N)
+0.1
-3.5
-0.1
-0.9
+0.2
pH
Growth at pH6 and (C) Trisodium citrate
-0.2
-0.9
-0.2
-2.2
-0.2
carbon source
Valeric (C)
-0.3
-0.7
-0.5
-2.2
-0.1
carbon source
ferulate (C); with MOPS
+0.2
-3.0
-0.2
-0.9
+0.3
nitrogen source
nitrogen source nitrite 0.5mM
+0.1
-3.6
+0.2
-0.0
-0.0
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM
+0.0
+0.1
-0.2
-3.1
-0.2
carbon source
acetate (C)
-0.1
-4.9
+0.2
+1.2
+0.4
carbon source
D-Glucose (C) with 20AA_mix_minus_Cys
-0.1
-3.4
-0.3
+0.5
+0.2
carbon source
carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM
+0.1
-3.2
+0.3
-0.1
-0.1
temperature shift
Temperature shift 10_to_30; with MOPS
-0.0
-3.5
-0.3
+0.6
+0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
-1.3
+0.3
-1.6
-0.7
temperature shift
Temperature shift 30_to_10
-0.2
-2.2
+0.1
-0.4
-0.2
carbon source
carbon source D-Glucose 55.5 mM
-0.2
-2.0
+0.1
-0.8
+0.2
temperature shift
Temperature shift 30_to_34; with MOPS
-0.1
-1.9
+0.3
-0.5
+0.3
phage
P. put South MOI 10
-0.2
+0.5
+0.1
-2.3
+0.1
carbon source
Hexanoic (C)
-0.1
-2.3
+0.0
+0.6
+0.0
nitrogen source
2,6-diaminopimelic (N)
+0.3
-0.5
+0.4
-2.0
+0.3
temperature shift
Temperature shift 30_to_25
+0.1
+0.8
+0.0
-2.3
+0.0
phage
P. putida South MOI 0.1
-0.2
-2.2
+0.2
+0.9
-0.0
temperature shift
Temperature shift 10_to_30; with MOPS
-0.1
+0.7
+0.1
-2.0
+0.1
nitrogen source
NAG (N); with MOPS
+0.2
+0.9
+0.2
-1.8
-0.6
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.0
+0.9
+0.3
-2.0
+0.3
carbon source
3-methyl-1-butanol 0.25 vol% (C)
-0.2
+1.2
+0.2
-1.6
-0.1
carbon source
Myristic (C)
-0.2
-1.5
-0.0
+1.6
-0.1
carbon source
L-Alanine (C)
-0.2
+2.0
+0.1
-1.3
+0.0
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
+0.1
+2.4
-0.1
-0.9
-0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.2
+1.5
-0.3
+1.7
-0.1
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