Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0651 and PP_5453 are separated by 833 nucleotidesPP_5453 and PP_0652 are separated by 64 nucleotidesPP_0652 and PP_0653 overlap by 4 nucleotidesPP_0653 and PP_0654 are separated by 151 nucleotides PP_0651: PP_0651 - Acetyltransferase, GNAT family, at 758,579 to 759,028 _0651 PP_5453: PP_5453 - conserved protein of unknown function, at 759,862 to 760,089 _5453 PP_0652: PP_0652 - Gluconate transporter, at 760,154 to 761,539 _0652 PP_0653: PP_0653 - conserved protein of unknown function, at 761,536 to 761,817 _0653 PP_0654: PP_0654 - putative malate dehydrogenase, at 761,969 to 762,901 _0654
Group Condition PP_0651 PP_5453 PP_0652 PP_0653 PP_0654
temperature Growth at 25C +0.0 -5.4 -0.2 -5.7 -0.2
carbon source D-Lysine 10 mM (C) -0.1 -0.9 +0.1 -5.6 -0.1
carbon source ferulate (C); with MOPS -0.0 -1.0 +0.1 -5.2 -0.3
nitrogen source Hydantoin (N) +0.1 -3.5 -0.1 -0.9 +0.2
pH Growth at pH6 and (C) Trisodium citrate -0.2 -0.9 -0.2 -2.2 -0.2
carbon source Valeric (C) -0.3 -0.7 -0.5 -2.2 -0.1
carbon source ferulate (C); with MOPS +0.2 -3.0 -0.2 -0.9 +0.3
nitrogen source nitrogen source nitrite 0.5mM +0.1 -3.6 +0.2 -0.0 -0.0
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM +0.0 +0.1 -0.2 -3.1 -0.2
carbon source acetate (C) -0.1 -4.9 +0.2 +1.2 +0.4
carbon source D-Glucose (C) with 20AA_mix_minus_Cys -0.1 -3.4 -0.3 +0.5 +0.2
carbon source carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM +0.1 -3.2 +0.3 -0.1 -0.1
temperature shift Temperature shift 10_to_30; with MOPS -0.0 -3.5 -0.3 +0.6 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 -1.3 +0.3 -1.6 -0.7
temperature shift Temperature shift 30_to_10 -0.2 -2.2 +0.1 -0.4 -0.2
carbon source carbon source D-Glucose 55.5 mM -0.2 -2.0 +0.1 -0.8 +0.2
temperature shift Temperature shift 30_to_34; with MOPS -0.1 -1.9 +0.3 -0.5 +0.3
phage P. put South MOI 10 -0.2 +0.5 +0.1 -2.3 +0.1
carbon source Hexanoic (C) -0.1 -2.3 +0.0 +0.6 +0.0
nitrogen source 2,6-diaminopimelic (N) +0.3 -0.5 +0.4 -2.0 +0.3
temperature shift Temperature shift 30_to_25 +0.1 +0.8 +0.0 -2.3 +0.0
phage P. putida South MOI 0.1 -0.2 -2.2 +0.2 +0.9 -0.0
temperature shift Temperature shift 10_to_30; with MOPS -0.1 +0.7 +0.1 -2.0 +0.1
nitrogen source NAG (N); with MOPS +0.2 +0.9 +0.2 -1.8 -0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 +0.9 +0.3 -2.0 +0.3
carbon source 3-methyl-1-butanol 0.25 vol% (C) -0.2 +1.2 +0.2 -1.6 -0.1
carbon source Myristic (C) -0.2 -1.5 -0.0 +1.6 -0.1
carbon source L-Alanine (C) -0.2 +2.0 +0.1 -1.3 +0.0
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. +0.1 +2.4 -0.1 -0.9 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.2 +1.5 -0.3 +1.7 -0.1
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