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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_0607 and PP_0608 overlap by 77 nucleotides
PP_0608 and PP_0609 overlap by 4 nucleotides
PP_0609 and PP_0610 overlap by 4 nucleotides
PP_0610 and PP_0611 are separated by 1 nucleotides
PP_0607: PP_0607 - putative Type IV pili biogenesis protein FimT, at 715,011 to 715,493
_0607
PP_0608: PP_0608 - putative type 4 fimbrial biogenesis protein, at 715,417 to 715,785
_0608
PP_0609: PP_0609 - conserved exported protein of unknown function, at 715,782 to 716,426
_0609
PP_0610: PP_0610 - conserved exported protein of unknown function, at 716,423 to 716,887
_0610
PP_0611: PP_0611 - Type IV pili biogenesis protein PilE, at 716,889 to 717,278
_0611
Group
Condition
PP
_0607
PP
_0608
PP
_0609
PP
_0610
PP
_0611
phage
P. putida Sci small MOI 0.1
-0.3
+0.7
+0.1
-5.5
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-3.6
-0.1
+0.2
-0.8
-0.2
carbon source
1,3-Butandiol (C)
-1.9
-0.6
-0.1
-0.0
-0.8
phage
P. putida Missley MOI 0.1
+0.0
+0.2
+0.2
-4.1
+0.2
carbon source
carbon source 3-O-methyl-glucose 50 mM
-1.1
-1.4
-0.2
-0.2
-0.5
micoeukaryotes
Tetrahymena sp., Mixed culture-2
-1.9
-0.7
+0.3
-0.8
+0.0
carbon source
carbon source 2-Deoxy-D-glucose 100 mM
-1.4
-0.6
-0.1
-1.0
+0.0
carbon source
L-Phenylalanine 5 mM (C)
-0.8
-1.2
-0.5
-0.5
+0.1
carbon source
carbon source 4-vinylphenol 0.1 mM
-1.2
-0.4
-0.2
-0.8
-0.2
nitrogen source
D-2-Aminobutyric (N)
-1.6
-0.3
-0.2
-0.3
-0.4
carbon source
D-Glucose (C)
-2.1
+0.0
-0.1
-0.3
-0.2
pH
pH 8
-1.2
-0.2
-0.3
-1.0
+0.2
carbon source
ferulate (C); with MOPS
-0.2
+0.6
-0.0
-1.1
-1.7
carbon source
ferulate (C); with MOPS
-0.5
-0.2
+0.2
+0.0
-1.7
nitrogen source
Ammonium chloride (N)
-1.9
-0.1
+0.1
-0.4
+0.2
stress
R2A with Fusaric 240 ug/mL
+0.3
-0.1
-0.0
-1.6
-0.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-1.4
-0.1
-0.2
-0.5
+0.3
pH
Valerolactone (C) pH 6.5
-2.2
-0.1
-0.0
+0.3
+0.0
phage
PUT 8 MOI 1
-1.2
+0.5
-0.5
+0.1
-0.6
pH
Growth at pH7 and (C) D-Glucose
-0.4
+0.4
+0.7
-0.9
-1.3
pH
Growth at pH6 and (C) Trisodium citrate
-0.8
+0.3
+0.3
-1.1
+0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.4
-0.6
+0.4
+0.3
+0.6
pH
Growth at pH6 and (C) D-Glucose
+1.1
-1.9
+0.3
-0.2
+0.0
r2a control with 0.2x vogels
R2A control with 0.2X Vogels
+0.7
-0.6
+0.1
-1.0
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.4
+0.6
-0.2
-0.9
+0.8
nitrogen source
2,6-diaminopimelic (N)
-0.6
+0.6
-0.3
+1.1
-0.4
stress
R2A with Polymyxin B sulfate 0.001 mg/ml
-0.8
+0.4
-0.0
+1.3
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-1.2
+0.1
+0.3
+0.7
+0.9
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.9
+0.9
-0.2
-0.7
-0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.8
+0.1
+0.6
+1.7
+0.7
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