Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0505 and PP_0506 are separated by 126 nucleotidesPP_0506 and PP_0507 overlap by 1 nucleotidesPP_0507 and PP_0508 are separated by 0 nucleotidesPP_0508 and PP_0509 are separated by 130 nucleotides PP_0505: PP_0505 - conserved protein of unknown function, at 594,249 to 594,761 _0505 PP_0506: PP_0506 - putative ABC efflux transporter, permease protein, at 594,888 to 596,153 _0506 PP_0507: PP_0507 - putative ABC efflux transporter, ATP-binding protein, at 596,153 to 596,863 _0507 PP_0508: PP_0508 - conserved exported protein of unknown function, at 596,864 to 597,445 _0508 PP_0509: PP_0509 - conserved protein of unknown function, at 597,576 to 597,920 _0509
Group Condition PP_0505 PP_0506 PP_0507 PP_0508 PP_0509
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM -1.2 -3.1 -3.1 -3.6 -0.6
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM -0.5 -2.4 -2.3 -2.8 -0.3
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM -0.8 -1.3 -2.0 -4.1 +0.4
carbon source carbon source Bisabolene 48.9 mM -1.2 -1.8 -2.4 -2.0 +0.4
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.2 -0.4 -0.7 -2.3 -1.5
carbon source carbon source Bisabolol 45 mM -1.1 -2.2 -1.4 -0.8 +0.8
pH pH 5 -0.2 -2.3 -1.5 -1.1 +0.7
carbon source L-tyrosine 5 mM (C) -0.2 -0.5 -0.9 -2.1 -0.7
pH pH 7 -0.1 -1.3 -1.3 -1.2 -0.1
stress Growth with Chloride 200 mM; with MOPS +0.4 -0.1 +0.2 -3.4 -0.9
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.2 -0.6 -0.1 -2.1 -0.4
pH Growth at pH9 and (C) Trisodium citrate -0.8 -0.1 -0.0 -0.3 -2.2
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.2 -0.6 -1.3 -1.6 +0.2
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM -0.0 -0.5 -0.9 -1.2 -0.6
pH pH 8 +0.0 -1.9 -0.4 -1.7 +0.7
carbon source L-tyrosine 5 mM (C) -0.4 -0.8 -1.0 -0.8 -0.2
carbon source carbon source 2-Deoxy-D-glucose 50 mM -0.4 -1.3 -1.2 -0.6 +0.5
carbon source carbon source 4-vinylphenol 0.01 mM +0.2 -1.1 -1.2 -1.1 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.6 +0.2 -0.7 -1.0 -0.4
pH pH 4 -0.1 -1.7 -0.8 -0.2 +0.3
pH pH 6 -0.1 -2.0 -0.6 +0.1 +0.3
pH Growth at pH7 and (C) D-Glucose -1.1 +0.0 +0.4 -1.5 +0.1
carbon source carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM -0.3 -1.0 -1.1 -0.6 +1.1
carbon source carbon source 4-vinylphenol 0.1 mM -0.8 -1.3 -0.5 -0.5 +1.1
pH pH 9 +0.2 -1.2 -1.2 -0.2 +0.8
carbon source carbon source 3-O-methyl-glucose 50 mM +0.0 -0.8 -0.9 -1.2 +1.3
carbon source carbon source Glycerol 108.6 mM -0.3 -1.5 -0.9 +0.2 +1.0
carbon source carbon source D-Glucose 55.5 mM +0.6 -0.5 -0.2 -1.6 +1.3
pH Valerolactone (C) pH 6.5 +0.5 -0.2 +0.2 -1.2 +0.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.2 +0.1 +0.2 +2.0 -0.8
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