Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0424 and PP_0425 are separated by 190 nucleotidesPP_0425 and PP_0426 are separated by 76 nucleotidesPP_0426 and PP_0427 are separated by 10 nucleotidesPP_0427 and PP_0428 are separated by 65 nucleotides PP_0424: PP_0424 - DNA-binding transcriptional dual regulator, at 512,709 to 513,353 _0424 PP_0425: PP_0425 - conserved protein of unknown function, at 513,544 to 513,966 _0425 PP_0426: PP_0426 - conserved membrane protein of unknown function, at 514,043 to 515,083 _0426 PP_0427: PP_0427 - 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, at 515,094 to 515,741 _0427 PP_0428: PP_0428 - aminoacyl-adenylate hydrolase/purine nucleoside phosphoramidase, at 515,807 to 516,145 _0428
Group Condition PP_0424 PP_0425 PP_0426 PP_0427 PP_0428
carbon source 4-Hydroxyvalerate (C) (40mM) +0.4 -3.3 -0.0 N.D. -0.4
carbon source p-Coumaric (C) -0.5 -0.7 +0.1 N.D. -1.8
carbon source Delta-Nonalactone (C) -0.2 -0.2 -0.0 N.D. -2.0
carbon source ferulate (C); with MOPS -0.1 -1.6 -0.1 N.D. +0.1
carbon source Trisodium citrate (C) +0.3 -2.0 -0.1 N.D. +0.1
carbon source p-Coumaric (C) -0.4 +0.1 -0.0 N.D. -1.5
temperature Growth at 30C; with MOPS -0.5 -1.1 -0.3 N.D. +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 -2.0 +0.2 N.D. +0.1
carbon source Valerolactone (C) -1.3 +0.3 -0.2 N.D. +0.1
nitrogen source D-2-Aminobutyric (N) -0.2 -0.6 +0.3 N.D. -0.5
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM +0.4 -1.2 -0.1 N.D. -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.6 -0.9 -0.0 N.D. -0.7
temperature shift Temperature shift 30_to_34; with MOPS +0.3 -1.1 +0.0 N.D. -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.5 -1.0 +0.2 N.D. +0.4
carbon source 1,3-Hexanediol (C) +0.7 +0.2 +0.1 N.D. -1.8
carbon source Tween 20 (C) -1.4 +0.4 +0.1 N.D. +0.2
carbon source Delta-Decalactone (C) +0.3 +0.1 +0.2 N.D. -1.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.8 -1.0 -0.0 N.D. -0.3
pH Growth at pH7 and (C) D-Glucose +0.8 -1.0 +0.2 N.D. -0.4
carbon source 1,3-Hexanediol (C) +0.7 +0.2 +0.1 N.D. -1.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X +0.6 -1.1 -0.0 N.D. +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.5 -0.9 +0.3 N.D. +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.8 -0.3 +0.3 N.D. -0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.6 +1.0 +0.3 N.D. -0.0
carbon source carbon source Bisabolene 48.9 mM +0.6 +0.5 +0.2 N.D. -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.5 +1.4 +0.0 N.D. +0.1
pH Growth at pH7 and (C) Trisodium citrate +0.7 +0.8 +0.1 N.D. -0.4
phage P. putida PUT 7 MOI 1 +0.6 -0.4 +0.6 N.D. +0.8
phage P. put South MOI 10 +0.4 +1.1 +0.3 N.D. +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.3 +2.0 -0.1 N.D. +0.3
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