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Protein
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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_0424 and PP_0425 are separated by 190 nucleotides
PP_0425 and PP_0426 are separated by 76 nucleotides
PP_0426 and PP_0427 are separated by 10 nucleotides
PP_0427 and PP_0428 are separated by 65 nucleotides
PP_0424: PP_0424 - DNA-binding transcriptional dual regulator, at 512,709 to 513,353
_0424
PP_0425: PP_0425 - conserved protein of unknown function, at 513,544 to 513,966
_0425
PP_0426: PP_0426 - conserved membrane protein of unknown function, at 514,043 to 515,083
_0426
PP_0427: PP_0427 - 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, at 515,094 to 515,741
_0427
PP_0428: PP_0428 - aminoacyl-adenylate hydrolase/purine nucleoside phosphoramidase, at 515,807 to 516,145
_0428
Group
Condition
PP
_0424
PP
_0425
PP
_0426
PP
_0427
PP
_0428
carbon source
4-Hydroxyvalerate (C) (40mM)
+0.4
-3.3
-0.0
N.D.
-0.4
carbon source
p-Coumaric (C)
-0.5
-0.7
+0.1
N.D.
-1.8
carbon source
Delta-Nonalactone (C)
-0.2
-0.2
-0.0
N.D.
-2.0
carbon source
ferulate (C); with MOPS
-0.1
-1.6
-0.1
N.D.
+0.1
carbon source
Trisodium citrate (C)
+0.3
-2.0
-0.1
N.D.
+0.1
carbon source
p-Coumaric (C)
-0.4
+0.1
-0.0
N.D.
-1.5
temperature
Growth at 30C; with MOPS
-0.5
-1.1
-0.3
N.D.
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.5
-2.0
+0.2
N.D.
+0.1
carbon source
Valerolactone (C)
-1.3
+0.3
-0.2
N.D.
+0.1
nitrogen source
D-2-Aminobutyric (N)
-0.2
-0.6
+0.3
N.D.
-0.5
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM
+0.4
-1.2
-0.1
N.D.
-0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days
+0.6
-0.9
-0.0
N.D.
-0.7
temperature shift
Temperature shift 30_to_34; with MOPS
+0.3
-1.1
+0.0
N.D.
-0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
-0.5
-1.0
+0.2
N.D.
+0.4
carbon source
1,3-Hexanediol (C)
+0.7
+0.2
+0.1
N.D.
-1.8
carbon source
Tween 20 (C)
-1.4
+0.4
+0.1
N.D.
+0.2
carbon source
Delta-Decalactone (C)
+0.3
+0.1
+0.2
N.D.
-1.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
+0.8
-1.0
-0.0
N.D.
-0.3
pH
Growth at pH7 and (C) D-Glucose
+0.8
-1.0
+0.2
N.D.
-0.4
carbon source
1,3-Hexanediol (C)
+0.7
+0.2
+0.1
N.D.
-1.3
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X
+0.6
-1.1
-0.0
N.D.
+0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.5
-0.9
+0.3
N.D.
+0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.8
-0.3
+0.3
N.D.
-0.3
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.6
+1.0
+0.3
N.D.
-0.0
carbon source
carbon source Bisabolene 48.9 mM
+0.6
+0.5
+0.2
N.D.
-0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.5
+1.4
+0.0
N.D.
+0.1
pH
Growth at pH7 and (C) Trisodium citrate
+0.7
+0.8
+0.1
N.D.
-0.4
phage
P. putida PUT 7 MOI 1
+0.6
-0.4
+0.6
N.D.
+0.8
phage
P. put South MOI 10
+0.4
+1.1
+0.3
N.D.
+0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+1.3
+2.0
-0.1
N.D.
+0.3
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