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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_0349 and PP_0350 are separated by 56 nucleotides
PP_0350 and PP_0351 are separated by 251 nucleotides
PP_0351 and PP_0352 overlap by 4 nucleotides
PP_0352 and PP_0353 are separated by 147 nucleotides
PP_0349: PP_0349 - putative Membrane protein, at 424,197 to 425,309
_0349
PP_0350: PP_0350 - Outer membrane ferrichrome-iron receptor, at 425,366 to 427,798
_0350
PP_0351: PP_0351 - putative Transmembrane sensor protein, at 428,050 to 429,006
_0351
PP_0352: PP_0352 - RNA polymerase sigma-70 factor, ECF subfamily, at 429,003 to 429,521
_0352
PP_0353: PP_0353 - Exonuclease, at 429,669 to 430,379
_0353
Group
Condition
PP
_0349
PP
_0350
PP
_0351
PP
_0352
PP
_0353
stress
MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM
-0.2
-0.0
-0.1
-0.6
-4.6
stress
MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM
-0.1
+0.0
-0.1
-0.0
-4.4
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
-0.4
+0.2
-1.8
-0.2
+0.2
stress
Geraniol 1 vol%
-0.3
-0.2
-0.8
-0.9
+0.1
nitrogen source
Propandiamine (N)
+0.0
+0.1
-0.5
+0.1
-1.8
carbon source
Myristic (C)
+0.2
-0.1
-0.3
-1.7
+0.2
nitrogen source
D-2-Aminobutyric (N)
+0.3
+0.1
-0.3
-0.3
-1.5
reactor
24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
+0.2
+0.1
-1.8
-0.6
+0.4
pH
Growth at pH7 and (C) D-Glucose
-0.0
+0.1
-0.3
-1.6
+0.4
carbon source
Acetate Carbon Source (5mM)
+0.0
-0.2
+0.0
+0.4
-1.6
reactor
40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
-0.4
+0.0
-1.4
+0.3
+0.1
carbon source
ferulate (C); with MOPS
-0.2
+0.0
+0.5
-1.4
-0.0
nitrogen source
D-2-Aminobutyric (N)
+0.4
+0.0
+0.3
-1.2
-0.5
stress
R2A with Fusaric 480 ug/mL
+0.4
-0.2
-0.7
-0.5
+0.3
carbon source
carbon source 4-vinylphenol 0.01 mM
-0.3
-0.3
-0.2
+1.1
+0.2
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
-0.2
+0.2
+1.1
-0.6
carbon source
butyrate (C)
+0.2
+0.2
-0.1
-0.5
+1.2
nitrogen source
2,6-diaminopimelic (N)
-0.3
-0.1
+0.2
+1.3
+0.1
carbon source
Levulinic Acid (C)
-0.1
+0.1
-0.2
-0.0
+1.7
carbon source
4-Hydroxyvalerate (C) (40mM)
-0.1
+0.1
-0.2
+0.6
+1.2
carbon source
Levulinic Acid (C)
-0.0
+0.1
-0.4
+0.4
+1.6
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
-0.2
+0.1
+0.8
+1.0
carbon source
4-Hydroxyvalerate (C) (40mM)
+0.0
+0.1
-0.4
+0.7
+1.5
carbon source
acetate (C)
+0.4
-0.3
+0.5
+1.1
+0.2
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.6
+0.4
-0.4
+0.6
+0.9
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.6
+0.2
+0.4
+0.6
+0.6
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
+0.2
+1.3
+0.8
+0.0
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.0
-0.1
+4.8
+0.4
+0.8
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.1
-0.1
+5.2
+0.2
+0.6
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.1
-0.0
+5.4
+0.8
+0.8
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