Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0333 and PP_0334 are separated by 149 nucleotidesPP_0334 and PP_0335 are separated by 428 nucleotidesPP_0335 and PP_0336 are separated by 3 nucleotidesPP_0336 and PP_0337 are separated by 168 nucleotides PP_0333: PP_0333 - conserved exported protein of unknown function, at 399,765 to 400,265 _0333 PP_0334: PP_0334 - transposase, at 400,415 to 401,473 _0334 PP_0335: PP_0335 - Glutathione S-transferase family protein, at 401,902 to 402,525 _0335 PP_0336: PP_0336 - Peptide methionine sulfoxide reductase MsrA, at 402,529 to 403,197 _0336 PP_0337: PP_0337 - Sensory box protein, at 403,366 to 406,056 _0337
Group Condition PP_0333 PP_0334 PP_0335 PP_0336 PP_0337
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.5 N.D. -0.6 -0.7 -0.3
pH Valerolactone (C) pH 6.5 +0.2 N.D. -0.6 -0.9 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.1 N.D. -1.0 -0.1 -0.2
pH Growth at pH7 and (C) D-Glucose +0.4 N.D. -0.9 -0.4 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.1 N.D. -1.3 +0.3 +0.1
pH Growth at pH7 and (C) D-Glucose -0.6 N.D. -0.7 +0.3 +0.0
pH Growth at pH6 and (C) Trisodium citrate +0.4 N.D. -0.9 -0.2 -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 N.D. +0.8 -1.0 -0.0
temperature Growth at 25C +0.4 N.D. -0.9 -0.0 +0.3
temperature shift Temperature shift 10_to_30 +0.3 N.D. -0.7 +0.3 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 N.D. +0.3 -0.3 +0.4
nitrogen source NAG (N); with MOPS -0.1 N.D. -0.6 +0.3 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 N.D. -0.2 -0.3 +0.5
nitrogen source Urea (N); with MOPS +0.9 N.D. +0.2 -0.6 +0.1
carbon source ferulate (C); with MOPS +0.9 N.D. -0.5 +0.3 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 N.D. +0.5 -0.1 +0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.4 N.D. +0.6 -0.3 +0.2
carbon source D-Lysine 10 mM (C) +0.5 N.D. +0.5 +0.2 -0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 N.D. +0.2 -0.1 +0.3
carbon source Delta-Undecalactone (C) +0.6 N.D. +0.5 +0.2 -0.1
carbon source ferulate (C); with MOPS +0.4 N.D. +0.3 -0.0 +0.6
carbon source D-Glucose (C) +0.3 N.D. +0.6 +0.3 +0.1
pH Growth at pH8 and (C) D-Glucose +0.9 N.D. +0.0 +0.5 -0.1
phage P. putida Sci small MOI 0.1 +0.8 N.D. +0.4 +0.2 -0.0
pH pH 5 +0.1 N.D. +0.6 +0.3 +0.4
phage P. putida Sci small MOI 0.1 +0.8 N.D. +0.3 +0.1 +0.2
stress Geraniol 1 vol% -0.1 N.D. +0.1 -0.1 +1.6
nitrogen source D-2-Aminobutyric (N) -0.2 N.D. +0.6 +0.7 +0.6
nitrogen source NAG (N); with MOPS +0.8 N.D. +0.6 +0.6 -0.3
pH Growth at pH6 and (C) D-Glucose +0.8 N.D. +0.7 +0.4 +0.1
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