Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0300 and PP_0301 are separated by 99 nucleotidesPP_0301 and PP_0302 are separated by 10 nucleotidesPP_0302 and PP_0303 are separated by 166 nucleotidesPP_0303 and PP_0304 are separated by 123 nucleotides PP_0300: PP_0300 - putative metallopeptidase, at 360,350 to 361,936 _0300 PP_0301: PP_0301 - betainyl-CoA thiolase, at 362,036 to 362,506 _0301 PP_0302: PP_0302 - L-carnitine 3-dehydrogenase (EC 1.1.1.108) (from data), at 362,517 to 363,482 _0302 PP_0303: PP_0303 - dehydrocarnitine cleavage enzyme, at 363,649 to 364,533 _0303 PP_0304: PP_0304 - Carnitine uptake ABC transporter, periplasmic component, at 364,657 to 365,601 _0304
Group Condition PP_0300 PP_0301 PP_0302 PP_0303 PP_0304
nitrogen source Carnitine Hydrochloride (N) +0.1 -3.4 -2.9 -3.4 -2.8
nitrogen source Carnitine Hydrochloride (N) +0.0 -2.6 -2.9 -2.8 -2.8
nitrogen source NAG (N); with MOPS -0.0 -0.5 -0.3 -2.4 -0.5
carbon source Valeric (C) +0.2 -1.3 +0.0 -1.1 -0.1
temperature shift Temperature shift 30_to_25; with MOPS +0.0 -0.5 -0.3 -0.7 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.0 +0.1 -0.5 -1.7 +0.2
pH Growth at pH7 and (C) Trisodium citrate -0.0 -0.5 -0.6 -1.0 +0.2
pH Growth at pH6 and (C) D-Glucose +0.3 -1.2 +0.2 -0.5 -0.6
carbon source Valerolactone (C) +0.4 -0.2 -0.3 -1.5 +0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.1 -0.6 +0.2 -0.6 -0.4
phage P. putida Sci small MOI 0.1 -0.1 -0.1 +0.3 -1.4 +0.2
mixed carbon source mixed (C)s Trisodium citrate 10 mM and Parachlorophenylalanine 10 mM +0.1 -0.2 +0.1 -1.2 +0.2
pH Growth at pH6 and (C) D-Glucose +0.1 -0.0 +0.4 -1.1 -0.4
temperature shift Temperature shift 10_to_30 +0.1 -0.4 +0.3 -0.7 -0.3
pH Valerolactone (C) pH 6.5 -0.3 +0.5 +0.2 -1.0 -0.2
nitrogen source D-2-Aminobutyric (N) +0.1 +0.7 +0.0 -1.4 -0.1
nitrogen source Propiolactam (N) +0.3 -0.5 +0.4 -1.0 +0.3
carbon source ferulate (C); with MOPS +0.1 -0.2 +0.3 -0.8 +0.3
carbon source ferulate (C); with MOPS +0.1 +0.2 +0.0 -1.1 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 +0.9 +0.4 -0.5 -0.3
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 +0.3 -0.1 +0.8 -0.5
pH pH 6 +0.3 +0.5 +0.1 -0.5 +0.3
nitrogen source nitrate (N) -0.2 +0.5 +0.4 -0.6 +0.6
nitrogen source D-2-Aminobutyric (N) -0.2 -0.2 -0.1 +0.8 +0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.0 -0.3 +0.2 +0.7 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.4 +0.3 -0.3 +0.5
pH pH 8 +0.2 +0.4 +0.4 +0.5 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.0 +1.0 +0.2 +0.4 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 -0.1 +0.1 +0.4 +0.7
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.1 +0.0 +0.8 +0.5
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