Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0278 and PP_0279 are separated by 392 nucleotidesPP_0279 and PP_0280 are separated by 128 nucleotidesPP_0280 and PP_0281 overlap by 1 nucleotidesPP_0281 and PP_0282 are separated by 54 nucleotides PP_0278: PP_0278 - conserved protein of unknown function, at 335,916 to 336,152 _0278 PP_0279: PP_0279 - conserved protein of unknown function, at 336,545 to 337,750 _0279 PP_0280: PP_0280 - Amino acid ABC transporter, permease protein, at 337,879 to 338,568 _0280 PP_0281: PP_0281 - Amino acid ABC transporter, permease protein, at 338,568 to 339,260 _0281 PP_0282: PP_0282 - L-arginine ABC transporter - periplasmic binding subunit, at 339,315 to 340,067 _0282
Group Condition PP_0278 PP_0279 PP_0280 PP_0281 PP_0282
pH Growth at pH7 and (C) D-Glucose -1.7 -0.5 -0.7 -0.1 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.9 -0.3 -0.7 -0.7 -0.1
stress Geraniol 1 vol% -0.2 -0.2 -0.7 -0.7 -1.0
carbon source Trisodium citrate (C) -0.8 -0.3 -1.5 +0.3 +0.1
nitrogen source L-Ornithine (N) -0.1 -0.2 -0.5 -0.9 -0.6
nitrogen source L-Ornithine (N) -0.2 -0.1 -0.6 -0.9 -0.4
phage moi 100 Time6-phageFRS -0.4 -0.2 -0.3 -0.5 -0.8
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.3 -0.1 -0.4 -0.1 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -1.0 -0.6 -0.5 +0.2 -0.1
stress R2A with Fusaric 480 ug/mL -0.8 -0.3 +0.2 +0.1 -1.2
carbon source 3-methyl-1-butanol (C) +0.1 +0.3 -0.8 -0.8 -0.8
stress R2A with Fusaric 480 ug/mL -0.1 -0.1 -0.4 +0.3 -1.4
stress R2A with Fusaric 480 ug/mL -0.5 +0.1 +0.1 +0.1 -1.5
nitrogen source Hippuric (N) +0.4 -0.3 -0.5 -0.7 -0.5
carbon source 1,3-Butandiol (C) -0.5 -0.4 +0.2 -0.7 +0.3
carbon source carbon source Bisabolene 48.9 mM -0.9 -0.5 +0.6 +0.3 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +1.1 -0.1 -0.0 -0.8 -0.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.4 -0.4 +0.7 -0.7 +0.1
carbon source carbon source 3-O-methyl-glucose 50 mM -0.8 -0.3 +0.4 +0.5 -0.4
carbon source carbon source D-Glucose 55.5 mM -0.3 -0.9 +0.1 +0.5 +0.3
stress Growth with Chloride 500 mM; with MOPS -1.1 +0.4 +0.0 +0.6 +0.2
pH pH 9 -1.1 -0.2 +0.5 +0.3 +0.6
carbon source ferulate (C); with MOPS -0.3 +0.9 -0.3 +0.5 -0.3
nitrogen source D-2-Aminobutyric (N) +1.0 -0.4 +0.4 -0.2 +0.1
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay -0.1 -0.3 +0.6 +0.7 +0.5
carbon source L-Lysine (C) -0.3 -0.3 +0.6 +0.7 +1.0
carbon source L-Lysine 10 mM (C) +0.1 -0.2 +0.8 +0.8 +0.7
micoeukaryotes C.elegans, mixed culture-2 +0.5 +0.0 +0.7 +0.3 +0.6
nitrogen source Propandiamine (N) +0.0 +0.4 +1.1 +0.8 +0.9
nitrogen source Propandiamine (N) +0.1 +0.3 +1.0 +0.9 +1.5
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