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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_0278 and PP_0279 are separated by 392 nucleotides
PP_0279 and PP_0280 are separated by 128 nucleotides
PP_0280 and PP_0281 overlap by 1 nucleotides
PP_0281 and PP_0282 are separated by 54 nucleotides
PP_0278: PP_0278 - conserved protein of unknown function, at 335,916 to 336,152
_0278
PP_0279: PP_0279 - conserved protein of unknown function, at 336,545 to 337,750
_0279
PP_0280: PP_0280 - Amino acid ABC transporter, permease protein, at 337,879 to 338,568
_0280
PP_0281: PP_0281 - Amino acid ABC transporter, permease protein, at 338,568 to 339,260
_0281
PP_0282: PP_0282 - L-arginine ABC transporter - periplasmic binding subunit, at 339,315 to 340,067
_0282
Group
Condition
PP
_0278
PP
_0279
PP
_0280
PP
_0281
PP
_0282
pH
Growth at pH7 and (C) D-Glucose
-1.7
-0.5
-0.7
-0.1
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.9
-0.3
-0.7
-0.7
-0.1
stress
Geraniol 1 vol%
-0.2
-0.2
-0.7
-0.7
-1.0
carbon source
Trisodium citrate (C)
-0.8
-0.3
-1.5
+0.3
+0.1
nitrogen source
L-Ornithine (N)
-0.1
-0.2
-0.5
-0.9
-0.6
nitrogen source
L-Ornithine (N)
-0.2
-0.1
-0.6
-0.9
-0.4
phage moi 100
Time6-phageFRS
-0.4
-0.2
-0.3
-0.5
-0.8
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.3
-0.1
-0.4
-0.1
-0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-1.0
-0.6
-0.5
+0.2
-0.1
stress
R2A with Fusaric 480 ug/mL
-0.8
-0.3
+0.2
+0.1
-1.2
carbon source
3-methyl-1-butanol (C)
+0.1
+0.3
-0.8
-0.8
-0.8
stress
R2A with Fusaric 480 ug/mL
-0.1
-0.1
-0.4
+0.3
-1.4
stress
R2A with Fusaric 480 ug/mL
-0.5
+0.1
+0.1
+0.1
-1.5
nitrogen source
Hippuric (N)
+0.4
-0.3
-0.5
-0.7
-0.5
carbon source
1,3-Butandiol (C)
-0.5
-0.4
+0.2
-0.7
+0.3
carbon source
carbon source Bisabolene 48.9 mM
-0.9
-0.5
+0.6
+0.3
-0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days
+1.1
-0.1
-0.0
-0.8
-0.9
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
-0.4
-0.4
+0.7
-0.7
+0.1
carbon source
carbon source 3-O-methyl-glucose 50 mM
-0.8
-0.3
+0.4
+0.5
-0.4
carbon source
carbon source D-Glucose 55.5 mM
-0.3
-0.9
+0.1
+0.5
+0.3
stress
Growth with Chloride 500 mM; with MOPS
-1.1
+0.4
+0.0
+0.6
+0.2
pH
pH 9
-1.1
-0.2
+0.5
+0.3
+0.6
carbon source
ferulate (C); with MOPS
-0.3
+0.9
-0.3
+0.5
-0.3
nitrogen source
D-2-Aminobutyric (N)
+1.0
-0.4
+0.4
-0.2
+0.1
reactor
72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay
-0.1
-0.3
+0.6
+0.7
+0.5
carbon source
L-Lysine (C)
-0.3
-0.3
+0.6
+0.7
+1.0
carbon source
L-Lysine 10 mM (C)
+0.1
-0.2
+0.8
+0.8
+0.7
micoeukaryotes
C.elegans, mixed culture-2
+0.5
+0.0
+0.7
+0.3
+0.6
nitrogen source
Propandiamine (N)
+0.0
+0.4
+1.1
+0.8
+0.9
nitrogen source
Propandiamine (N)
+0.1
+0.3
+1.0
+0.9
+1.5
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