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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_0252 and PP_0254 are separated by 1868 nucleotides
PP_0254 and PP_0255 are separated by 56 nucleotides
PP_0255 and PP_0256 are separated by 196 nucleotides
PP_0256 and PP_0257 are separated by 5 nucleotides
PP_0252: PP_0252 - 33 kDa chaperonin, at 306,086 to 306,985
_0252
PP_0254: PP_0254 - conserved protein of unknown function, at 308,854 to 309,294
_0254
PP_0255: PP_0255 - conserved exported protein of unknown function, at 309,351 to 309,833
_0255
PP_0256: PP_0256 - putative oxidoreductase, at 310,030 to 312,375
_0256
PP_0257: PP_0257 - protein sulfur transferase, at 312,381 to 313,211
_0257
Group
Condition
PP
_0252
PP
_0254
PP
_0255
PP
_0256
PP
_0257
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
N.D.
-2.4
+0.4
+0.0
-0.2
nitrogen source
Urea (N)
N.D.
-2.2
-0.1
-0.0
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
N.D.
-1.5
+0.4
+0.0
-0.9
carbon source
Delta-Decalactone (C)
N.D.
-1.5
+0.3
-0.1
-0.7
carbon source
Delta-Nonalactone (C)
N.D.
-0.2
+0.0
-0.1
-1.7
phage
P. putida Sci small MOI 0.1
N.D.
-0.5
-0.0
-0.2
-1.2
pH
Growth at pH7 and (C) Trisodium citrate
N.D.
-0.5
-0.6
-0.4
-0.4
nitrogen source
NAG (N); with MOPS
N.D.
-0.7
-0.2
-0.2
-0.8
phage
P. putida Sci Big MOI 0.1
N.D.
-0.2
-0.3
-0.3
-0.9
carbon source
ferulate (C); with MOPS
N.D.
-1.2
+0.1
-0.0
-0.7
phage
P. putida Sci small MOI 0.1
N.D.
-0.6
-0.0
-0.5
-0.5
nitrogen source
no extra (N); with MOPS
N.D.
-1.7
-0.0
+0.4
-0.2
nitrogen source
Ammonium chloride (N)
N.D.
-1.1
-0.2
-0.4
+0.3
carbon source
ferulate (C); with MOPS
N.D.
-0.7
-0.2
+0.4
-0.6
carbon source
Trisodium citrate (C)
N.D.
-0.9
+0.2
+0.2
-0.6
carbon source
Nonanoic (C)
N.D.
-1.0
-0.1
+0.4
-0.3
phage
P. putida South MOI 10
N.D.
-1.1
-0.3
+0.1
+0.3
nitrogen source
nitrogen source nitrite 1mM
N.D.
-1.2
-0.2
+0.4
+0.3
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-0.1
-0.1
+0.7
-1.3
pH
Growth at pH7 and (C) Trisodium citrate
N.D.
-1.0
-0.4
+0.4
+0.3
nitrogen source
D-2-Aminobutyric (N)
N.D.
-1.8
-0.1
+0.3
+1.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
+0.4
+0.1
-0.1
-1.2
temperature shift
Temperature shift 30_to_21; with MOPS
N.D.
+0.4
+0.2
+0.3
-1.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
N.D.
+1.0
-0.3
+0.1
-0.7
carbon source
Acetate Carbon Source (20mM)
N.D.
+1.1
-0.3
-0.1
-0.3
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
N.D.
+0.5
+0.4
+0.5
-0.8
pH
Growth at pH6 and (C) D-Glucose
N.D.
-0.3
-0.2
+0.3
+0.9
carbon source
Tween 20 (C)
N.D.
+1.0
-0.6
+0.2
+0.4
phage
PUT 8 MOI 1
N.D.
+1.0
-0.3
-0.0
+0.4
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+1.0
-0.3
+0.2
+0.4
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