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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_0205 and PP_0206 are separated by 62 nucleotides
PP_0206 and PP_0207 are separated by 119 nucleotides
PP_0207 and PP_0208 are separated by 15 nucleotides
PP_0208 and PP_0209 overlap by 1 nucleotides
PP_0205: PP_0205 - putative Oxidoreductase, at 256,315 to 258,039
_0205
PP_0206: PP_0206 - Ferredoxin, 4Fe-4S, at 258,102 to 258,347
_0206
PP_0207: PP_0207 - putative Nitrate ABC transporter, periplasmic nitrate-binding protein, at 258,467 to 259,873
_0207
PP_0208: PP_0208 - putative Nitrate ABC transporter, permease protein, at 259,889 to 260,671
_0208
PP_0209: PP_0209 - ATP-binding taurine transporter subunit, at 260,671 to 261,522
_0209
Group
Condition
PP
_0205
PP
_0206
PP
_0207
PP
_0208
PP
_0209
phage
P. putida Sci small MOI 0.1
-0.6
-0.8
-0.5
-0.8
-0.7
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days
-0.2
-2.6
-0.0
+0.1
-0.2
carbon source
D-Glucose (C)
+0.2
-3.5
+0.1
+0.0
+0.5
temperature shift
Temperature shift 30_to_25; with MOPS
-0.1
-1.9
-0.1
-0.1
-0.1
carbon source
Valerolactone (C)
+0.1
-2.2
-0.0
+0.0
-0.3
temperature shift
Temperature shift 30_to_34; with MOPS
+0.1
-1.7
-0.2
-0.1
-0.2
nitrogen source
D-2-Aminobutyric (N)
-0.1
-1.0
-0.1
-0.0
-0.6
carbon source
Trisodium citrate (C)
-0.1
-0.3
-0.5
-0.2
-0.7
phage
P. putida Sci Big MOI 0.1
-0.5
+0.0
-0.5
-0.3
-0.5
pH
Growth at pH8 and (C) D-Glucose
-0.1
-1.2
-0.1
-0.1
-0.2
phage
P. putida PUT 7 MOI 1
-0.4
+0.4
-0.3
-0.7
-0.7
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.2
-0.8
-0.1
-0.3
-0.7
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.1
-1.3
-0.0
+0.0
-0.4
r2a control with 0.2% methanol
R2A control with 0.2% methanol
+0.2
-1.3
+0.0
-0.0
-0.4
pH
Growth at pH6 and (C) D-Glucose
+0.4
-2.7
-0.1
+0.1
+0.7
phage
P. putida Missley MOI 0.1
-0.4
+0.3
-0.6
-0.4
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.0
-1.6
+0.1
+0.1
+0.4
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.1
-0.8
+0.1
+0.2
-0.5
temperature
Growth at 25C
+0.1
-1.0
-0.4
+0.0
+0.5
phage
P. putida Sci small MOI 0.1
+0.0
+0.6
-0.5
-0.2
-0.6
carbon source
4-Methyl-2-oxopentanoic (C)
+0.2
-1.3
+0.1
-0.2
+0.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.1
+0.4
+0.1
+0.3
-1.2
nitrogen source
Propandiamine (N)
+0.2
-1.2
+0.3
+0.2
+0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.4
-1.0
+0.1
+0.0
+0.3
carbon source
acetate (C)
+0.1
+0.7
-0.3
-0.2
-0.5
pH
Valerolactone (C) pH 6.5
+0.2
+1.0
+0.1
-0.3
-0.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
+0.1
+1.1
+0.2
-0.2
+0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.3
+0.5
+0.1
+0.3
+0.7
carbon source
carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM
+0.1
+1.1
+0.3
+0.2
-0.3
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
+1.3
+0.1
+0.0
-0.1
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