Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0198 and PP_0199 are separated by 48 nucleotidesPP_0199 and PP_0200 are separated by 8 nucleotidesPP_0200 and PP_0201 are separated by 65 nucleotidesPP_0201 and PP_5432 are separated by 61 nucleotides PP_0198: PP_0198 - putative transporter, at 248,420 to 249,052 _0198 PP_0199: PP_0199 - putative membrane protease family, stomatin, at 249,101 to 249,847 _0199 PP_0200: PP_0200 - putative membrane bound peptidase, nefD, at 249,856 to 251,202 _0200 PP_0201: PP_0201 - conserved protein of unknown function, at 251,268 to 251,759 _0201 PP_5432: PP_5432 - conserved protein of unknown function, at 251,821 to 252,063 _5432
Group Condition PP_0198 PP_0199 PP_0200 PP_0201 PP_5432
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -2.5 +0.1 +0.1 -0.2 N.D.
phage P. putida Missley MOI 0.1 -0.8 -0.2 -1.0 -0.4 N.D.
nitrogen source nitrate (N) -1.2 -0.1 +0.1 -0.4 N.D.
carbon source ferulate (C); with MOPS -1.4 +0.4 +0.2 +0.0 N.D.
pH Growth at pH7 and (C) D-Glucose -0.4 -0.8 +0.3 +0.1 N.D.
stress MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL +0.3 -0.7 -0.2 -0.2 N.D.
stress Growth with Chloride 200 mM -0.4 -0.8 +0.2 +0.2 N.D.
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM -1.0 +0.1 -0.1 +0.3 N.D.
pH Growth at pH7 and (C) D-Glucose -1.1 +0.2 +0.2 -0.0 N.D.
pH pH 6 +0.5 -0.4 +0.4 -0.9 N.D.
carbon source D-Glucose (C) +0.4 -0.3 +0.1 -0.6 N.D.
pH pH 4 -0.3 -0.6 +0.2 +0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.9 -0.2 +0.9 +0.2 N.D.
carbon source 3-methyl-1-butanol 0.1 vol% (C) +0.8 -0.0 -0.2 -0.3 N.D.
carbon source Myristic (C) +0.7 -0.8 +0.2 +0.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.4 -0.3 -0.3 -0.3 N.D.
stress Growth with Chloride 500 mM; with MOPS +0.9 -0.4 +0.1 -0.2 N.D.
carbon source acetate (C) +0.9 +0.0 -0.2 -0.4 N.D.
carbon source carbon source 4-vinylphenol 0.1 mM +1.0 +0.4 -0.3 -0.4 N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.7 -0.4 +0.2 +0.5 N.D.
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay +0.9 +0.3 +0.0 -0.2 N.D.
pH pH 8 +1.1 +0.1 +0.1 -0.3 N.D.
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. +1.2 -0.3 -0.0 +0.1 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.7 +0.2 +0.3 -0.2 N.D.
carbon source carbon source D-Glucose 55.5 mM +0.8 -0.2 +0.1 +0.3 N.D.
carbon source ferulate (C); with MOPS +1.4 -0.4 +0.3 +0.0 N.D.
micoeukaryotes C.elegans, mixed culture-2 +0.4 +0.1 +0.4 +0.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 +0.7 -0.4 +0.6 N.D.
nitrogen source Propandiamine (N) +0.9 -0.0 +0.2 +0.3 N.D.
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. +1.1 +0.4 +0.0 +0.1 N.D.
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