Fitness for 5 genes in Pseudomonas putida KT2440

Top 30 experiments (either direction), sorted by average fitness

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Group	Condition	PP_0196	PP_0197	PP_0198	PP_0199	PP_0200
temperature shift	Temperature shift 30_to_25; with MOPS	+0.1	-2.0	-0.7	+0.0	-0.1
phage	P. putida Missley MOI 0.1	-0.4	+0.0	-0.8	-0.2	-1.0
agar plate interaction	Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant	-0.0	+0.0	-2.5	+0.1	+0.1
soil	Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs	-0.5	-1.3	+0.7	-0.2	-0.3
nitrogen source	2,6-diaminopimelic (N)	-0.5	-1.0	-0.1	+0.3	-0.1
mixed carbon source	mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM	+0.3	-0.6	-1.0	+0.1	-0.1
carbon source	L-Leucine (C)	-1.2	+0.3	+0.1	-0.1	-0.3
nitrogen source	nitrate (N)	+0.5	-0.6	-1.2	-0.1	+0.1
pH	Growth at pH7 and (C) D-Glucose	+0.1	-0.5	-1.1	+0.2	+0.2
carbon source	Myristic (C)	+0.4	-1.6	+0.7	-0.8	+0.2
pH	pH 4	+0.3	-0.6	-0.3	-0.6	+0.2
carbon source	ferulate (C); with MOPS	-0.3	+0.2	-1.4	+0.4	+0.2
soil	Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs	-0.3	+0.5	-0.3	-0.6	-0.1
in planta	Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days	-0.6	-0.7	+1.4	-0.3	-0.3
nitrogen source	D-2-Aminobutyric (N)	-0.7	+1.1	-0.5	-0.0	-0.3
in planta	Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days	-0.3	+0.6	-0.7	-0.3	+0.3
stress	Growth with Chloride 200 mM	+0.1	+0.6	-0.4	-0.8	+0.2
in planta	Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days	+0.3	-0.0	-0.9	-0.2	+0.9
soil	Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs	-0.2	-0.7	+0.3	+0.7	+0.0
stress	Growth with Chloride 500 mM; with MOPS	+0.1	-0.4	+0.9	-0.4	+0.1
in planta	Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days	-0.3	+0.2	+0.6	+0.7	-0.4
in planta	Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days	+0.1	+1.2	+0.2	-0.3	-0.1
in planta	Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days	-0.0	+0.7	+0.7	-0.4	+0.2
micoeukaryotes	Acanthamoeba sp., muxed culture-2	+0.6	+0.7	-0.4	+0.2	+0.0
carbon source	carbon source 4-vinylphenol 0.1 mM	+0.6	-0.2	+1.0	+0.4	-0.3
reactor	48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.	+0.6	-0.1	+1.2	-0.3	-0.0
supernatant	Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X	-0.2	+0.6	+0.7	+0.2	+0.3
carbon source	ferulate (C); with MOPS	+0.4	+0.3	+1.4	-0.4	+0.3
carbon source	4-Hydroxyvalerate (C) (40mM)	+0.4	+0.9	+0.2	+0.3	+0.2
reactor	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.	+0.3	+0.4	+1.1	+0.4	+0.0