Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_r01 and PP_5SB are separated by 105 nucleotidesPP_5SB and PP_0160 are separated by 64 nucleotidesPP_0160 and PP_0161 are separated by 93 nucleotidesPP_0161 and PP_0162 are separated by 4 nucleotides PP_r01: PP_r01 - 5S ribosomal RNA, at 181,667 to 181,786 _r01 PP_5SB: PP_5SB - 5S ribosomal RNA, at 181,892 to 182,011 _5SB PP_0160: PP_0160 - putative ferrioxamine receptor, at 182,076 to 184,562 _0160 PP_0161: PP_0161 - putative transmembrane sensor, at 184,656 to 185,612 _0161 PP_0162: PP_0162 - RNA polymerase sigma-70 factor, ECF subfamily, at 185,617 to 186,138 _0162
Group Condition PP_r01 PP_5SB PP_0160 PP_0161 PP_0162
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. -2.3 -0.2 +0.1 +0.1
no stress control Growth with no stress N.D. -1.8 -0.2 -0.3 +0.1
stress Growth with Chloride 500 mM; with MOPS N.D. -1.3 -0.1 -0.6 -0.0
pH Growth at pH7 and (C) D-Glucose N.D. -2.0 -0.0 +0.1 -0.0
stress Butyrolactam 2 vol% N.D. -1.0 -0.2 -0.2 -0.4
r2a control with 0.2x vogels R2A control with 0.2X Vogels N.D. -1.4 +0.1 -0.2 -0.2
pH Growth at pH7 and (C) Trisodium citrate N.D. -1.5 -0.1 -0.3 +0.3
carbon source Valeric (C) N.D. -0.7 +0.1 -0.4 -0.5
carbon source L-Glutamic (C) N.D. -1.5 -0.1 +0.1 -0.1
nitrogen source nitrite (N) N.D. -1.6 -0.1 +0.1 -0.0
temperature Growth at 34C N.D. -1.3 -0.1 -0.3 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.6 +0.1 +0.0 +0.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.2 +0.2 -0.2 -0.1
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM N.D. -1.2 -0.1 +0.2 -0.1
temperature shift Temperature shift 30_to_34; with MOPS N.D. -1.1 -0.1 -0.3 +0.4
temperature shift Temperature shift 30_to_34; with MOPS N.D. -1.3 +0.0 -0.1 +0.3
temperature Growth at 30C N.D. -1.3 +0.0 +0.3 -0.1
nitrogen source Urea (N) N.D. -1.2 -0.2 +0.0 +0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.5 +0.1 +0.2 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs N.D. -1.3 +0.1 +0.3 -0.0
nitrogen source Urea (N); with MOPS N.D. -1.1 +0.0 +0.2 +0.4
phage P. put PUT 10 MOI 0.1 N.D. -1.0 +0.1 +0.5 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs N.D. -0.8 +0.1 +0.6 +0.5
phage P. putida Sci small MOI 0.1 N.D. -0.3 +0.5 +0.7 +0.2
phage P. putida Sci Big MOI 0.1 N.D. +0.6 +0.3 +0.6 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +1.2 +0.2 -0.0 +0.8
phage P. putida compost MOI 0.1 N.D. +0.8 +0.5 +0.6 +0.4
phage P. putida Sci small MOI 0.1 N.D. +0.4 +0.7 +1.0 +0.4
phage P. putida PUT 7 MOI 1 N.D. +1.0 +1.0 +1.3 +1.0
phage P. putida Missley MOI 0.1 N.D. +1.7 +1.1 +1.2 +1.0
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