Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0120 and PP_0121 are separated by 49 nucleotidesPP_0121 and PP_0122 are separated by 69 nucleotidesPP_0122 and PP_0123 are separated by 75 nucleotidesPP_0123 and PP_0124 are separated by 370 nucleotides PP_0120: PP_0120 - putative zinc ABC transporter, at 125,182 to 126,090 _0120 PP_0121: PP_0121 - homoserine kinase, at 126,140 to 127,090 _0121 PP_0122: PP_0122 - conserved exported protein of unknown function, at 127,160 to 127,447 _0122 PP_0123: PP_0123 - DNA polymerase I, 5, at 127,523 to 130,270 _0123 PP_0124: PP_0124 - probable GTP-binding protein, at 130,641 to 131,273 _0124
Group Condition PP_0120 PP_0121 PP_0122 PP_0123 PP_0124
temperature Growth at 30C; with MOPS -0.3 N.D. -6.9 -0.1 N.D.
temperature shift Temperature shift 30_to_25 -0.3 N.D. -6.6 +0.3 N.D.
carbon source 3-methyl-1-butanol 0.1 vol% (C) +0.3 N.D. -2.5 -2.8 N.D.
carbon source carbon source Bisabolene 48.9 mM -4.6 N.D. +0.0 -0.2 N.D.
pH Growth at pH6 and (C) Trisodium citrate -0.2 N.D. -4.4 -0.2 N.D.
carbon source carbon source Bisabolol 45 mM -4.7 N.D. -0.3 +0.2 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.6 N.D. -2.2 -2.0 N.D.
carbon source carbon source D-Glucose 55.5 mM -3.1 N.D. -1.6 +0.2 N.D.
micoeukaryotes Tetrahymena sp., Mixed culture-2 -0.3 N.D. -3.3 -0.9 N.D.
pH Growth at pH9 and (C) Trisodium citrate -0.4 N.D. -3.5 -0.4 N.D.
carbon source 3-methyl-1-butanol (C) -0.3 N.D. -0.5 -3.4 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.5 N.D. -0.5 -3.0 N.D.
carbon source ferulate (C); with MOPS -1.5 N.D. -2.7 +0.2 N.D.
nitrogen source Hippuric (N) -2.2 N.D. -1.2 -0.6 N.D.
carbon source Lignin Monomers (C) -0.6 N.D. -1.2 -2.0 N.D.
stress Formaldehyde 0.03125 vol% -1.3 N.D. +0.3 -2.8 N.D.
nitrogen source Urea (N) -0.4 N.D. -3.0 -0.1 N.D.
carbon source BC Lignin Sorghum 20 vol% (C) -0.6 N.D. -0.2 -2.8 N.D.
temperature shift Temperature shift 30_to_25; with MOPS -1.1 N.D. -2.8 +0.5 N.D.
carbon source 3-methyl-1-butanol (C) +0.1 N.D. -0.3 -3.1 N.D.
pH Growth at pH6 and (C) D-Glucose -0.3 N.D. -3.1 +0.4 N.D.
nitrogen source D-2-Aminobutyric (N) -2.3 N.D. -0.8 +0.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.5 N.D. -2.6 -1.4 N.D.
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -2.0 N.D. -0.5 +1.4 N.D.
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. -2.5 N.D. +0.4 +1.3 N.D.
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -2.5 N.D. +0.2 +1.6 N.D.
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. -0.9 N.D. -0.2 +2.4 N.D.
phage P. putida South MOI 1 -0.7 N.D. +0.4 +2.4 N.D.
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay -0.3 N.D. +1.0 +2.1 N.D.
carbon source L-Leucine (C) +3.5 N.D. +0.4 +0.6 N.D.
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