Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0051 and PP_0052 are separated by 222 nucleotidesPP_0052 and PP_0053 are separated by 40 nucleotidesPP_0053 and PP_0054 overlap by 43 nucleotidesPP_0054 and PP_0055 overlap by 12 nucleotides PP_0051: PP_0051 - putative sigma-54 dependent transcriptional regulator, at 59,316 to 60,608 _0051 PP_0052: PP_0052 - beta-lactamase domain protein, putative hydrolase, at 60,831 to 61,715 _0052 PP_0053: PP_0053 - putative sulfide:quinone oxidoreductase, at 61,756 to 63,024 _0053 PP_0054: PP_0054 - conserved membrane protein of unknown function, at 62,982 to 63,839 _0054 PP_0055: PP_0055 - transcriptional regulator, at 63,828 to 64,715 _0055
Group Condition PP_0051 PP_0052 PP_0053 PP_0054 PP_0055
carbon source 3-Hydroxypropionate (C) -0.1 +0.2 +0.0 -0.2 -6.8
carbon source DL-3-Hydroxyisobutyric (C) +0.2 +0.2 +0.0 +0.1 -7.1
carbon source DL-3-Hydroxyisobutyric (C) +0.3 +0.1 -0.0 -0.1 -6.3
carbon source 3-Hydroxypropionate (C) +0.1 +0.1 +0.0 +0.1 -4.7
phage P. putida Sci small MOI 0.1 +0.0 -0.2 -0.2 -1.8 -0.3
nitrogen source NAG (N); with MOPS +0.3 -0.6 +0.1 -0.7 -1.2
carbon source Tween 20 (C) -0.1 -0.8 -0.1 -0.3 -0.4
micoeukaryotes Acanthamoeba sp., muxed culture-2 -0.5 -0.5 -0.2 +0.2 -0.3
carbon source DL-3-Hydroxybutyric (C) -0.1 +0.0 +0.1 -0.0 -1.3
carbon source DL-3-Hydroxybutyric (C) -0.1 +0.2 +0.1 -0.1 -1.4
pH Growth at pH8 and (C) Trisodium citrate +0.2 -0.3 -0.2 -0.8 +0.1
pH Growth at pH7 and (C) D-Glucose +0.1 +0.4 -0.1 -0.6 -0.5
pH Growth at pH6 and (C) D-Glucose -0.1 +0.6 -0.0 +0.0 -1.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.4 -0.5 -0.1 -0.1 +0.6
carbon source ferulate (C); with MOPS +0.4 -0.1 +0.2 -0.7 -0.1
carbon source ferulate (C); with MOPS +0.6 -0.4 -0.0 +0.3 -0.8
nitrogen source NAG (N); with MOPS +0.2 +0.4 +0.4 -0.8 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -0.4 -0.6 +0.3 +0.5 +0.1
carbon source Valeric (C) -0.5 -0.2 -0.2 +0.1 +0.7
temperature shift Temperature shift 30_to_21; with MOPS +0.2 -0.2 +0.1 +0.5 -0.6
carbon source 1,3-Butandiol (C) -0.2 +0.3 -0.2 +0.6 -0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 +0.4 -0.3 -0.5 +0.5
carbon source carbon source D-Glucose 55.5 mM +0.3 -0.6 -0.2 +0.4 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +0.3 -0.4 +0.7 -0.1
carbon source L-Alanine (C) -0.7 -0.1 +0.3 +0.1 +0.7
carbon source carbon source Bisabolene 48.9 mM +0.6 -0.3 -0.2 +0.1 +0.5
pH pH 8 +0.8 -0.3 +0.1 +0.4 -0.1
nitrogen source D-2-Aminobutyric (N) +0.3 +0.3 -0.4 +0.3 +0.5
nitrogen source nitrogen source nitrite 1mM +0.5 -0.2 +0.1 +0.4 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.3 +0.0 -0.1 +0.8 +0.3
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