Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0023 and PP_0024 are separated by 122 nucleotidesPP_0024 and PP_0025 are separated by 442 nucleotidesPP_0025 and PP_0026 are separated by 288 nucleotidesPP_0026 and PP_0027 are separated by 67 nucleotides PP_0023: PP_0023 - conserved hypothetical protein, at 27,599 to 27,727 _0023 PP_0024: PP_0024 - putative membrane-associated metal-dependent hydrolase, at 27,850 to 29,547 _0024 PP_0025: PP_0025 - hypothetical protein, at 29,990 to 30,142 _0025 PP_0026: PP_0026 - putative cobalt/cadmium/zinc exporter, at 30,431 to 31,336 _0026 PP_0027: PP_0027 - conserved membrane protein of unknown function, at 31,404 to 31,844 _0027
Group Condition PP_0023 PP_0024 PP_0025 PP_0026 PP_0027
carbon source D-Glucose (C); with MOPS N.D. -0.1 -5.2 +0.0 -0.3
nitrogen source Urea (N) N.D. -0.2 -5.2 -0.0 +0.1
carbon source butyrate (C) N.D. -0.0 -4.8 -0.0 -0.2
carbon source D-Galacturonic Acid (C) N.D. -0.0 -4.3 -0.1 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.0 -4.5 +0.3 -0.0
pH Growth at pH7 and (C) D-Glucose N.D. -0.3 -3.1 -0.1 -0.0
temperature shift Temperature shift 10_to_25; with MOPS N.D. -0.1 -2.9 -0.2 -0.1
phage P. putida Sci Big MOI 0.1 N.D. -0.3 -2.4 -0.1 -0.0
carbon source Crotonic (C) N.D. +0.0 -2.6 -0.1 -0.1
pH Growth at pH9 and (C) Trisodium citrate N.D. -0.2 -2.1 -0.2 -0.4
temperature shift Temperature shift 10_to_34; with MOPS N.D. -0.1 -2.5 -0.1 -0.1
carbon source ferulate (C); with MOPS N.D. -0.0 -2.6 -0.1 -0.0
carbon source 2,3-Butanediol (C) N.D. +0.1 -2.6 -0.0 -0.2
carbon source L-Leucine (C) N.D. -0.2 -2.4 -0.1 +0.1
pH Growth at pH6 and (C) D-Glucose N.D. -0.1 -2.6 +0.1 +0.2
carbon source Valerolactone (C) N.D. +0.2 -3.2 +0.3 +0.2
pH Growth at pH6 and (C) Trisodium citrate N.D. -0.1 -2.4 +0.1 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.1 -2.3 +0.1 -0.1
carbon source Heptanoic (C) N.D. -0.2 -2.0 -0.1 -0.1
nitrogen source NAG (N); with MOPS N.D. +0.0 -2.1 -0.1 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. +0.1 -2.9 +0.0 +0.4
no stress control Growth with no stress N.D. -0.4 -1.7 -0.3 +0.1
stress Growth with Chloride 500 mM; with MOPS N.D. -0.1 -1.7 -0.0 -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.0 -1.7 -0.1 -0.3
nitrogen source no extra (N); with MOPS N.D. -0.2 -1.8 +0.1 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.1 -2.1 +0.0 -0.1
nitrogen source 2,6-diaminopimelic (N) N.D. +0.3 -1.6 +0.2 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.0 +1.6 +0.2 +0.3
stress R2A with Polymyxin B sulfate 0.001 mg/ml N.D. +0.1 +1.9 +0.1 +0.1
stress R2A with Polymyxin B sulfate 0.001 mg/ml N.D. -0.0 +3.0 +0.1 -0.3
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