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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_04835 and PS417_04840 are separated by 38 nucleotides
PS417_04840 and PS417_04845 are separated by 74 nucleotides
PS417_04845 and PS417_04850 are separated by 836 nucleotides
PS417_04850 and PS417_04855 are separated by 169 nucleotides
PS417_04835: PS417_04835 - hemolysin D, at 1,072,984 to 1,074,150
_04835
PS417_04840: PS417_04840 - glycosyl transferase, at 1,074,189 to 1,075,679
_04840
PS417_04845: PS417_04845 - GDP-mannose dehydrogenase, at 1,075,754 to 1,077,070
_04845
PS417_04850: PS417_04850 - hypothetical protein, at 1,077,907 to 1,078,686
_04850
PS417_04855: PS417_04855 - polysaccharide deacetylase, at 1,078,856 to 1,079,980
_04855
Group
Condition
PS417
_04835
PS417
_04840
PS417
_04845
PS417
_04850
PS417
_04855
phage
JP1 MOI 10
-1.6
-0.7
-0.3
+0.0
-0.0
phage
JP1 MOI 1
-1.5
-0.4
-0.4
-0.2
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.8
+0.1
+0.2
-0.3
-0.3
phage
P. simiae OR Antrim MOI 10
-1.5
-0.3
+0.1
-0.2
-0.1
phage
JP1 MOI 10
-0.7
-0.2
+0.2
-0.7
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-1.3
+0.1
+0.2
-0.3
+0.0
carbon source
2-Deoxy-D-Ribose 10 mM (C)
-1.0
-0.1
-0.5
+0.1
+0.4
seeds
Growth on radish seeds for 72 hours
-1.1
+0.4
-0.4
-0.3
+0.5
agar plate interaction control
Taped volatile agar plate with no fungus
-0.9
-0.0
-0.4
+0.4
+0.1
liquid stress
Scopoletin 3 mM; liquid stress
-0.8
-0.0
+0.4
-0.4
-0.0
stress
Cisplatin 0.01 mg/ml
-1.1
+0.1
+0.2
-0.1
+0.1
soil
soil sample 7; outgrowth in LB
-0.5
+0.4
+0.2
-0.4
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-1.2
+0.0
+0.3
+0.3
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.9
-0.2
-0.5
+0.1
+0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.7
-0.7
-0.1
-0.0
+0.4
stress
Phosphomycin 0.003 mg/ml
-0.5
+0.5
+0.7
+0.0
+0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.4
-0.5
+0.4
+0.3
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.0
-0.1
-0.2
-0.0
+0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+1.2
-0.2
+0.1
-0.1
+0.0
phage
P. simiae OR1 MOI 0.1
+1.0
+0.1
+0.3
-0.3
+0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+1.0
-0.2
+0.4
-0.1
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.7
-0.2
+0.4
+0.4
+0.1
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
+0.6
-0.4
+0.3
+0.6
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.3
+0.4
+0.7
+0.2
+0.1
motility
inner cut, LB soft agar motility assay
+1.0
+0.3
+0.5
-0.2
+0.2
stress
Phosphomycin 0.001 mg/ml
+0.4
+0.6
+0.8
-0.1
+0.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+1.2
+0.4
+0.1
+0.1
+0.2
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+1.3
-0.1
+0.3
+0.5
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+1.2
+0.5
+0.2
+0.1
+0.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+3.0
-0.1
-0.1
-0.0
+0.1
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