Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_04795 and PS417_04800 are separated by 16 nucleotidesPS417_04800 and PS417_04805 are separated by 11 nucleotidesPS417_04805 and PS417_04810 are separated by 218 nucleotidesPS417_04810 and PS417_04815 are separated by 8 nucleotides PS417_04795: PS417_04795 - alginate O-acetyltransferase, at 1,062,199 to 1,062,855 _04795 PS417_04800: PS417_04800 - alginate O-acetyltransferase, at 1,062,872 to 1,064,056 _04800 PS417_04805: PS417_04805 - poly(beta-D-mannuronate) O-acetylase, at 1,064,068 to 1,065,600 _04805 PS417_04810: PS417_04810 - poly(beta-D-mannuronate) lyase, at 1,065,819 to 1,066,928 _04810 PS417_04815: PS417_04815 - alginate O-acetyltransferase, at 1,066,937 to 1,068,385 _04815
Group Condition PS417_04795 PS417_04800 PS417_04805 PS417_04810 PS417_04815
phage P. simiae Grant Run MOI 0.1 -0.6 -1.6 -0.8 -0.3 -0.7
phage P. simiae OR Antrim MOI 10 -0.2 -2.5 -0.3 -0.1 -0.5
soil soil sample 5; outgrowth in LB -0.6 -2.4 +0.0 +0.1 -0.1
phage JP1 MOI 10 -0.6 -1.2 -0.3 -0.5 +0.1
seeds Growth on radish seeds for 72 hours -0.6 +0.3 -0.4 -1.4 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.8 -0.2 -0.2 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -0.7 -0.0 -1.3 +0.2
agar plate interaction control Taped volatile agar plate with no fungus -0.5 -0.7 -0.3 -0.3 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.4 -1.0 -0.3 +0.2 +0.2
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 -1.0 +0.5 +0.0 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 +0.5 -0.1 -0.8 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 +0.9 -0.3 -0.8 -0.2
phage P. simiae Grant Run MOI 0.1 -0.2 +0.8 -0.6 -0.2 -0.5
seeds Growth on radish seeds for 72 hours +0.2 -1.3 +0.2 +0.1 +0.5
phage JP1 MOI 10 -0.2 +1.0 -0.4 -0.8 +0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.1 -1.2 +0.4 +0.3 +0.6
carbon source 2'-Deoxyinosine 5 mM (C) -0.8 +0.8 -0.2 +0.1 +0.2
phage JP1 MOI 0.1 -0.6 +0.8 -0.4 -0.0 +0.5
phage P. simiae ORA MOI 0.1 -0.5 +0.5 -0.3 +0.2 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.7 -0.7 +0.2 +0.6 -0.3
phage P. simiae Grant Run MOI 10 -0.1 -0.3 -0.2 +1.1 +0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.4 -0.6 +0.3 +0.4 +0.5
stress Gentamicin 0.008 mg/ml +0.4 +0.6 +0.3 -0.4 +0.4
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.8 +1.3 -0.3 +0.0 -0.0
stress Phosphomycin 0.002 mg/ml +0.4 +0.9 +0.1 +0.2 +0.6
carbon source L-Valine (C) +0.6 +1.0 +0.1 +0.7 -0.1
stress methylglyoxal 0.032 vol% +0.8 +0.4 -0.1 +0.7 +0.7
stress Phosphomycin 0.003 mg/ml +0.5 +0.9 +0.2 +0.3 +0.7
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.1 +1.5 +0.0 +0.6 +0.6
stress Phosphomycin 0.001 mg/ml +0.6 +1.3 +0.5 +0.4 +1.1
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