Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_04520 and PS417_04525 are separated by 260 nucleotidesPS417_04525 and PS417_04530 are separated by 30 nucleotidesPS417_04530 and PS417_04535 are separated by 85 nucleotidesPS417_04535 and PS417_04540 are separated by 258 nucleotides PS417_04520: PS417_04520 - membrane protein, at 998,673 to 999,257 _04520 PS417_04525: PS417_04525 - 16S rRNA methyltransferase, at 999,518 to 1,000,516 _04525 PS417_04530: PS417_04530 - glycerate dehydrogenase, at 1,000,547 to 1,001,512 _04530 PS417_04535: PS417_04535 - lysine transporter LysE, at 1,001,598 to 1,002,230 _04535 PS417_04540: PS417_04540 - long-chain fatty acid--CoA ligase, at 1,002,489 to 1,004,171 _04540
Group Condition PS417_04520 PS417_04525 PS417_04530 PS417_04535 PS417_04540
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x N.D. +0.0 +0.6 -3.3 +0.1
phage P. simiae OR1 MOI 0.1 N.D. -0.4 -1.5 -0.1 -0.3
phage JP1 MOI 0.1 N.D. -0.8 -0.2 -0.7 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.2 -0.8 -0.7 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.0 -1.3 +0.6 -0.3
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x N.D. +0.3 -0.8 -0.5 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.3 -1.1 +0.5 +0.3
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x N.D. +0.6 -0.1 -0.9 +0.3
carbon source Carbon source L-Citrulline 10 mM N.D. -0.3 +0.8 -0.5 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.7 -1.0 +0.6 -0.1
stress methylglyoxal 0.032 vol% N.D. -0.9 +1.0 +0.6 -0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.9 +1.4 -0.2 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.1 -0.5 +1.0 -0.1
seeds Growth on radish seeds for 72 hours N.D. +0.5 -0.7 +0.6 +0.0
carbon source L-Valine (C) N.D. -0.5 +0.5 +1.0 -0.3
soil soil sample 6; outgrowth in LB N.D. +0.8 -0.8 +1.0 -0.2
phage P. simiae OR Antrim MOI 0.1 N.D. -0.4 +1.0 +0.2 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.7 -0.3 +0.6 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. -0.3 +1.1 +0.6 -0.2
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.6 +1.3 -0.4 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.7 +0.8 -0.3 +0.1
stress Gentamicin 0.008 mg/ml N.D. +1.8 -0.9 +0.5 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.5 -0.6 +1.4 +0.2
soil soil sample 2; outgrowth in LB N.D. +0.5 +1.3 -0.2 +0.0
nitrogen source Adenine (N) N.D. +0.9 +0.6 +0.4 -0.2
motility inner cut, LB soft agar motility assay N.D. +0.4 +0.6 +0.7 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.5 +0.9 +0.7 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.6 +0.6 +0.8 +0.1
seeds Growth on radish seeds for 24 hours N.D. +0.8 +0.8 +1.1 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.4 +1.2 +1.0 +0.1
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