Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_04220 and PS417_04225 overlap by 4 nucleotidesPS417_04225 and PS417_04230 are separated by 58 nucleotidesPS417_04230 and PS417_04235 are separated by 157 nucleotidesPS417_04235 and PS417_04240 are separated by 10 nucleotides PS417_04220: PS417_04220 - 4-carboxymuconolactone decarboxylase, at 946,407 to 946,787 _04220 PS417_04225: PS417_04225 - conjugal transfer protein TraR, at 946,784 to 947,830 _04225 PS417_04230: PS417_04230 - lipoprotein, at 947,889 to 948,260 _04230 PS417_04235: PS417_04235 - glutamyl-tRNA amidotransferase, at 948,418 to 949,863 _04235 PS417_04240: PS417_04240 - glutamyl-tRNA amidotransferase, at 949,874 to 951,325 _04240
Group Condition PS417_04220 PS417_04225 PS417_04230 PS417_04235 PS417_04240
carbon source L-Leucine (C) -4.7 -0.7 +0.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.1 -1.8 -0.3 N.D. N.D.
root root sample 6; outgrowth in LB -2.4 -0.1 -0.4 N.D. N.D.
carbon source L-Valine (C) -2.4 -0.6 +0.1 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.3 -0.8 -0.8 N.D. N.D.
soil soil sample 6; outgrowth in LB -1.5 -0.2 -1.0 N.D. N.D.
rhizosphere rhizosphere sample 1; outgrowth in LB -2.5 +0.0 +0.0 N.D. N.D.
seeds Growth on radish seeds for 24 hours -1.0 -0.7 -0.6 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.9 -0.7 -0.6 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.9 +0.4 -0.3 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.5 -0.6 +0.2 N.D. N.D.
stress Neomycin 0.04 mg/ml +0.4 -1.7 -0.2 N.D. N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -1.8 -0.2 +0.5 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X -2.0 +0.5 -0.0 N.D. N.D.
nophagecontrol Only library -1.2 -0.6 +0.4 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -2.0 -0.3 +1.2 N.D. N.D.
soil soil sample 7; outgrowth in LB +0.9 -1.6 -0.4 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.2 +0.8 -0.4 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.4 +0.8 -0.1 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.5 -0.2 +0.9 N.D. N.D.
solid stress Fraxetin 3 mM; solid stress -1.5 +0.7 +0.1 N.D. N.D.
phage P. simiae OR1 MOI 0.1 -1.2 -0.2 +0.8 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -2.0 +1.8 +0.4 N.D. N.D.
phage JP1 MOI 0.1 -0.7 -0.2 +1.3 N.D. N.D.
phage JP1 MOI 10 -0.9 +0.8 +0.7 N.D. N.D.
stress methylglyoxal 0.032 vol% -1.0 +1.0 +0.8 N.D. N.D.
phage P. simiae Grant Run MOI 0.1 +1.6 -0.4 +0.5 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 +1.8 +0.9 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.9 +1.2 +0.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +3.2 -0.4 +0.5 N.D. N.D.
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