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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_04220 and PS417_04225 overlap by 4 nucleotides
PS417_04225 and PS417_04230 are separated by 58 nucleotides
PS417_04230 and PS417_04235 are separated by 157 nucleotides
PS417_04235 and PS417_04240 are separated by 10 nucleotides
PS417_04220: PS417_04220 - 4-carboxymuconolactone decarboxylase, at 946,407 to 946,787
_04220
PS417_04225: PS417_04225 - conjugal transfer protein TraR, at 946,784 to 947,830
_04225
PS417_04230: PS417_04230 - lipoprotein, at 947,889 to 948,260
_04230
PS417_04235: PS417_04235 - glutamyl-tRNA amidotransferase, at 948,418 to 949,863
_04235
PS417_04240: PS417_04240 - glutamyl-tRNA amidotransferase, at 949,874 to 951,325
_04240
Group
Condition
PS417
_04220
PS417
_04225
PS417
_04230
PS417
_04235
PS417
_04240
carbon source
L-Leucine (C)
-4.7
-0.7
+0.3
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.1
-1.8
-0.3
N.D.
N.D.
root
root sample 6; outgrowth in LB
-2.4
-0.1
-0.4
N.D.
N.D.
carbon source
L-Valine (C)
-2.4
-0.6
+0.1
N.D.
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-1.3
-0.8
-0.8
N.D.
N.D.
soil
soil sample 6; outgrowth in LB
-1.5
-0.2
-1.0
N.D.
N.D.
rhizosphere
rhizosphere sample 1; outgrowth in LB
-2.5
+0.0
+0.0
N.D.
N.D.
seeds
Growth on radish seeds for 24 hours
-1.0
-0.7
-0.6
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.9
-0.7
-0.6
N.D.
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-1.9
+0.4
-0.3
N.D.
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-1.5
-0.6
+0.2
N.D.
N.D.
stress
Neomycin 0.04 mg/ml
+0.4
-1.7
-0.2
N.D.
N.D.
supernatant control
Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
-1.8
-0.2
+0.5
N.D.
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
-2.0
+0.5
-0.0
N.D.
N.D.
nophagecontrol
Only library
-1.2
-0.6
+0.4
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-2.0
-0.3
+1.2
N.D.
N.D.
soil
soil sample 7; outgrowth in LB
+0.9
-1.6
-0.4
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-1.2
+0.8
-0.4
N.D.
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-1.4
+0.8
-0.1
N.D.
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-1.5
-0.2
+0.9
N.D.
N.D.
solid stress
Fraxetin 3 mM; solid stress
-1.5
+0.7
+0.1
N.D.
N.D.
phage
P. simiae OR1 MOI 0.1
-1.2
-0.2
+0.8
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-2.0
+1.8
+0.4
N.D.
N.D.
phage
JP1 MOI 0.1
-0.7
-0.2
+1.3
N.D.
N.D.
phage
JP1 MOI 10
-0.9
+0.8
+0.7
N.D.
N.D.
stress
methylglyoxal 0.032 vol%
-1.0
+1.0
+0.8
N.D.
N.D.
phage
P. simiae Grant Run MOI 0.1
+1.6
-0.4
+0.5
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
+1.8
+0.9
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.9
+1.2
+0.3
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+3.2
-0.4
+0.5
N.D.
N.D.
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