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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_04115 and PS417_04120 are separated by 24 nucleotides
PS417_04120 and PS417_04125 are separated by 79 nucleotides
PS417_04125 and PS417_04130 are separated by 148 nucleotides
PS417_04130 and PS417_04135 are separated by 145 nucleotides
PS417_04115: PS417_04115 - alpha/beta hydrolase, at 925,288 to 925,923
_04115
PS417_04120: PS417_04120 - tryptophan--tRNA ligase, at 925,948 to 927,303
_04120
PS417_04125: PS417_04125 - ATPase, at 927,383 to 928,477
_04125
PS417_04130: PS417_04130 - AraC family transcriptional regulator, at 928,626 to 929,621
_04130
PS417_04135: PS417_04135 - acyl-CoA dehydrogenase, at 929,767 to 930,903
_04135
Group
Condition
PS417
_04115
PS417
_04120
PS417
_04125
PS417
_04130
PS417
_04135
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.0
N.D.
N.D.
-0.3
-0.1
phage
JP1 MOI 10
-1.2
N.D.
N.D.
-0.3
+0.2
phage
P. simiae ORA MOI 0.1
-0.6
N.D.
N.D.
-0.4
-0.2
solid stress
Scopoletin 3 mM; solid stress
-0.6
N.D.
N.D.
-0.1
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.6
N.D.
N.D.
+0.1
+0.4
stress
LB_noSalt with Chloride 1100 mM
-1.1
N.D.
N.D.
+0.0
-0.1
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.5
N.D.
N.D.
-0.1
-0.5
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.8
N.D.
N.D.
-0.1
-0.2
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.5
N.D.
N.D.
+0.1
-0.5
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.8
N.D.
N.D.
+0.1
-0.2
agar plate interaction control
Taped volatile agar plate with no fungus
-1.1
N.D.
N.D.
+0.1
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.9
N.D.
N.D.
-0.1
+0.2
carbon source
Carbon source L-Ornithine 10 mM
-0.6
N.D.
N.D.
-0.3
+0.2
stress
LB_noSalt with Chloride 1100 mM
-0.9
N.D.
N.D.
+0.1
+0.1
stress
Choline chloride 300 mM
-0.6
N.D.
N.D.
+0.2
-0.2
carbon source
L-Valine (C)
-0.7
N.D.
N.D.
-0.3
+0.3
stress
Gentamicin 0.008 mg/ml
-1.1
N.D.
N.D.
+0.2
+0.2
stress
Doxycycline hyclate 0.001 mg/ml
-0.8
N.D.
N.D.
+0.2
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.9
N.D.
N.D.
+0.3
+0.1
rhizosphere
rhizosphere sample 2; outgrowth in LB
-0.1
N.D.
N.D.
+0.5
-0.7
phage
JP1 MOI 1
-0.5
N.D.
N.D.
+0.4
-0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.7
N.D.
N.D.
+0.4
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.5
N.D.
N.D.
-0.1
+0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.6
N.D.
N.D.
+0.2
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.5
N.D.
N.D.
+0.2
+0.3
soil
soil sample 2; outgrowth in LB
+0.5
N.D.
N.D.
+0.4
-0.4
carbon source
Deoxyribonucleic from herring sperm 5 mg/mL (C)
+0.8
N.D.
N.D.
+0.1
-0.2
stress
Phosphomycin 0.001 mg/ml
+0.8
N.D.
N.D.
+0.2
-0.0
stress
methylglyoxal 0.032 vol%
+0.4
N.D.
N.D.
+0.5
+0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.6
N.D.
N.D.
+0.2
+0.6
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