Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_03865 and PS417_03870 are separated by 103 nucleotidesPS417_03870 and PS417_03875 overlap by 4 nucleotidesPS417_03875 and PS417_03880 are separated by 75 nucleotidesPS417_03880 and PS417_03885 overlap by 4 nucleotides PS417_03865: PS417_03865 - hypothetical protein, at 868,456 to 869,085 _03865 PS417_03870: PS417_03870 - hypothetical protein, at 869,189 to 869,656 _03870 PS417_03875: PS417_03875 - membrane protein, at 869,653 to 870,342 _03875 PS417_03880: PS417_03880 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 870,418 to 870,633 _03880 PS417_03885: PS417_03885 - DNA gyrase inhibitor, at 870,630 to 870,830 _03885
Group Condition PS417_03865 PS417_03870 PS417_03875 PS417_03880 PS417_03885
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.2 -0.2 -2.1 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.2 -2.0 N.D. N.D.
motility inner cut, LB soft agar motility assay -0.3 -0.6 -0.9 N.D. N.D.
stress Doxycycline hyclate 0.001 mg/ml -0.7 -0.3 -0.9 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.5 -0.8 -0.5 N.D. N.D.
stress Phosphomycin 0.002 mg/ml -0.1 +0.3 -2.0 N.D. N.D.
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x -0.6 +0.1 -1.2 N.D. N.D.
soil soil sample 5; outgrowth in LB +0.3 -1.3 -0.5 N.D. N.D.
liquid stress Fraxetin 2 mM; liquid stress +0.2 +0.0 -1.7 N.D. N.D.
stress Gentamicin 0.008 mg/ml -0.5 +0.5 -1.4 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.3 +0.4 -1.1 N.D. N.D.
phage P. simiae OR Antrim MOI 1 +0.8 +0.4 -2.1 N.D. N.D.
supernatant control Vogels_fungal_media 0.1X +0.5 -0.9 -0.4 N.D. N.D.
phage P. simiae OR Antrim MOI 10 -0.9 +0.5 -0.2 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 -1.0 +0.2 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.7 -0.8 +0.2 N.D. N.D.
carbon source L-Valine (C) +0.3 -0.8 +0.9 N.D. N.D.
phage JP1 MOI 10 +0.5 +0.7 -0.6 N.D. N.D.
rhizosphere rhizosphere sample 8; outgrowth in LB -0.5 -0.1 +1.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +0.3 +1.2 N.D. N.D.
soil soil sample 7; outgrowth in LB -0.2 +0.5 +1.0 N.D. N.D.
solid stress 80% Methanol 2 mM; solid stress -0.3 +0.5 +1.3 N.D. N.D.
seeds Growth on radish seeds for 24 hours -0.1 +0.1 +1.5 N.D. N.D.
seeds Growth on radish seeds for 72 hours -0.3 +1.0 +0.7 N.D. N.D.
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI +0.4 +0.7 +0.8 N.D. N.D.
seeds Growth on radish seeds for 72 hours +0.5 +0.3 +1.2 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 +0.7 +1.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.8 +1.1 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.9 +0.4 +1.1 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 +0.1 +1.9 N.D. N.D.
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