Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_03730 and PS417_03735 are separated by 85 nucleotidesPS417_03735 and PS417_03740 are separated by 91 nucleotidesPS417_03740 and PS417_03745 are separated by 255 nucleotidesPS417_03745 and PS417_03750 are separated by 253 nucleotides PS417_03730: PS417_03730 - phosphate acetyltransferase, at 844,773 to 846,872 _03730 PS417_03735: PS417_03735 - hypothetical protein, at 846,958 to 847,149 _03735 PS417_03740: PS417_03740 - peptidylprolyl isomerase, at 847,241 to 847,726 _03740 PS417_03745: PS417_03745 - 30S ribosomal protein S20, at 847,982 to 848,260 _03745 PS417_03750: PS417_03750 - membrane protein, at 848,514 to 850,052 _03750
Group Condition PS417_03730 PS417_03735 PS417_03740 PS417_03745 PS417_03750
nitrogen source Urea (N) -0.1 N.D. -5.1 N.D. N.D.
nitrogen source Inosine (N) -0.4 N.D. -3.7 N.D. N.D.
carbon source L-Leucine (C) -1.7 N.D. -0.1 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 N.D. -1.4 N.D. N.D.
phage JP1 MOI 10 -0.6 N.D. -1.0 N.D. N.D.
carbon source L-Leucine (C) -1.6 N.D. +0.0 N.D. N.D.
phage JP1 MOI 1 -0.6 N.D. -0.9 N.D. N.D.
stress Gentamicin 0.008 mg/ml +0.1 N.D. -1.5 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 N.D. -0.9 N.D. N.D.
solid stress Fraxetin 2 mM; solid stress -0.4 N.D. -1.0 N.D. N.D.
stress Neomycin 0.04 mg/ml -0.0 N.D. -1.4 N.D. N.D.
phage JP1 MOI 0.1 -0.9 N.D. -0.4 N.D. N.D.
solid stress Fraxetin 2 mM; solid stress -0.6 N.D. -0.7 N.D. N.D.
soil soil sample 4; outgrowth in LB -0.8 N.D. -0.4 N.D. N.D.
carbon source L-Valine (C) +0.6 N.D. -1.4 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.0 N.D. +0.2 N.D. N.D.
stress Doxycycline hyclate 0.001 mg/ml +0.3 N.D. -1.0 N.D. N.D.
seeds Growth on radish seeds for 72 hours -1.1 N.D. +0.6 N.D. N.D.
solid stress Fraxetin 3 mM; solid stress +0.7 N.D. -1.0 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -1.1 N.D. +0.9 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.6 N.D. +0.6 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 N.D. +0.7 N.D. N.D.
carbon source L-Valine (C) +1.3 N.D. -1.0 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.5 N.D. +0.7 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.8 N.D. +1.1 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 N.D. +1.2 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +1.1 N.D. -0.2 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 N.D. +1.0 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.8 N.D. +0.5 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.7 N.D. +0.9 N.D. N.D.
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