Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_03690 and PS417_03695 are separated by 14 nucleotidesPS417_03695 and PS417_03700 are separated by 125 nucleotidesPS417_03700 and PS417_03705 are separated by 110 nucleotidesPS417_03705 and PS417_03710 are separated by 101 nucleotides PS417_03690: PS417_03690 - NAD-dependent dehydratase, at 834,433 to 835,332 _03690 PS417_03695: PS417_03695 - ferrochelatase, at 835,347 to 836,372 _03695 PS417_03700: PS417_03700 - TonB-dependent receptor, at 836,498 to 838,594 _03700 PS417_03705: PS417_03705 - 2-alkenal reductase, at 838,705 to 839,859 _03705 PS417_03710: PS417_03710 - metal-binding protein, at 839,961 to 840,719 _03710
Group Condition PS417_03690 PS417_03695 PS417_03700 PS417_03705 PS417_03710
carbon source octanoate (C) +0.0 N.D. -0.1 -1.4 -0.5
phage P. simiae Grant Run MOI 0.1 -0.1 N.D. -0.5 -1.0 -0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.0 N.D. -0.1 -1.1 -0.5
carbon source octanoate (C) +0.0 N.D. -0.1 -1.2 -0.3
stress Polymyxin B sulfate 0.006 mg/ml -0.1 N.D. +0.1 -1.2 -0.2
liquid stress 80% Methanol 3 mM; liquid stress -0.2 N.D. -0.0 -1.4 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.6 N.D. -0.1 -1.0 +0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.1 N.D. -0.2 -1.8 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 N.D. +0.2 -0.5 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.2 N.D. +0.0 -1.1 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 N.D. -0.0 -1.5 +0.6
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.0 N.D. +0.3 -1.6 +0.2
stress LB_noSalt with Chloride 1100 mM -0.0 N.D. -0.0 -1.4 +0.4
stress LB_noSalt with Chloride 1100 mM -0.0 N.D. +0.0 -1.5 +0.6
carbon source L-Valine (C) -0.0 N.D. +0.2 -1.4 +0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.2 N.D. +0.1 -1.2 +0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.3 N.D. +0.1 -1.3 +0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.2 N.D. +0.2 -1.1 +0.2
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI -0.3 N.D. +0.1 -0.8 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 N.D. -0.1 -0.9 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 N.D. +0.4 -0.9 +0.3
agar plate interaction control Taped volatile agar plate with no fungus +0.3 N.D. +0.0 -1.3 +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 N.D. +0.4 -0.5 +0.5
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 N.D. +0.3 +1.1 -0.3
rhizosphere rhizosphere sample 1; outgrowth in LB -0.2 N.D. -0.2 +0.5 +0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.0 N.D. -0.4 +0.8 +0.8
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2 N.D. +0.3 +0.7 +0.4
soil soil sample 5; outgrowth in LB -0.1 N.D. -0.1 +1.0 +0.5
root root sample 6; outgrowth in LB -0.2 N.D. +0.2 +1.5 +0.1
carbon source Trisodium citrate (C) -0.0 N.D. +0.5 +1.0 +0.3
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