Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_03620 and PS417_03625 are separated by 112 nucleotidesPS417_03625 and PS417_03630 are separated by 136 nucleotidesPS417_03630 and PS417_03635 are separated by 137 nucleotidesPS417_03635 and PS417_03640 overlap by 8 nucleotides PS417_03620: PS417_03620 - glutamate racemase, at 822,680 to 823,474 _03620 PS417_03625: PS417_03625 - lipid A 3-O-deacylase, at 823,587 to 824,105 _03625 PS417_03630: PS417_03630 - hypothetical protein, at 824,242 to 824,463 _03630 PS417_03635: PS417_03635 - membrane protein, at 824,601 to 825,080 _03635 PS417_03640: PS417_03640 - cyclopropane-fatty-acyl-phospholipid synthase, at 825,073 to 826,344 _03640
Group Condition PS417_03620 PS417_03625 PS417_03630 PS417_03635 PS417_03640
solid stress Fraxetin 3 mM; solid stress N.D. -4.1 -0.6 N.D. -0.1
solid stress Fraxetin 3 mM; solid stress N.D. -2.3 -0.5 N.D. -0.1
carbon source octanoate (C) N.D. -1.8 -0.7 N.D. -0.2
solid stress Fraxetin 3 mM; solid stress N.D. -2.6 +0.2 N.D. -0.1
phage P. simiae OR Antrim MOI 10 N.D. +0.1 -1.6 N.D. -0.8
carbon source L-Valine (C) N.D. -0.5 -0.7 N.D. -0.5
solid stress Fraxetin 3 mM; solid stress N.D. -2.1 +0.6 N.D. -0.0
carbon source octanoate (C) N.D. -1.1 -0.3 N.D. -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.4 -1.0 N.D. +0.0
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.5 -0.7 N.D. -0.0
solid stress Fraxetin 2 mM; solid stress N.D. -1.0 -0.2 N.D. +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.4 -0.8 N.D. +0.1
solid stress Fraxetin 2 mM; solid stress N.D. -1.0 +0.2 N.D. -0.1
phage JP1 MOI 1 N.D. +0.1 -1.4 N.D. +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.3 -0.7 N.D. +0.3
phage P. simiae ORA MOI 0.1 N.D. +0.5 -0.7 N.D. +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.1 -0.7 N.D. +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.5 +0.8 N.D. -0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. +0.8 -0.4 N.D. -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.4 +1.1 N.D. -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. +0.9 -0.3 N.D. +0.2
seeds Growth on radish seeds for 72 hours N.D. -0.5 +0.5 N.D. +0.9
stress methylglyoxal 0.032 vol% N.D. -0.5 +1.3 N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.7 +0.6 N.D. -0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +0.8 +0.1 N.D. +0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. +1.0 +0.0 N.D. +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.5 +0.7 N.D. +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.2 +0.9 N.D. +0.5
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +1.6 -0.2 N.D. +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.3 +1.3 N.D. +0.5
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