Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_03535 and PS417_03540 are separated by 2 nucleotidesPS417_03540 and PS417_03545 overlap by 8 nucleotidesPS417_03545 and PS417_03550 are separated by 6 nucleotidesPS417_03550 and PS417_03555 are separated by 473 nucleotides PS417_03535: PS417_03535 - type III secretion protein, at 807,504 to 808,115 _03535 PS417_03540: PS417_03540 - type III secretion protein, at 808,118 to 808,675 _03540 PS417_03545: PS417_03545 - type III secretion protein, at 808,668 to 809,489 _03545 PS417_03550: PS417_03550 - hypothetical protein, at 809,496 to 809,858 _03550 PS417_03555: PS417_03555 - ATPase AAA, at 810,332 to 811,258 _03555
Group Condition PS417_03535 PS417_03540 PS417_03545 PS417_03550 PS417_03555
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 -0.1 +0.2 -1.2 +0.1
soil soil sample 5; outgrowth in LB -0.3 +0.2 +0.2 -1.2 -0.0
stress Paraquat dichloride 0.008 mg/ml +0.2 +0.1 +0.0 -1.3 -0.1
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -0.6 +0.0 +0.2 -0.6 +0.2
carbon source L-Valine (C) -0.4 -0.2 +0.5 -1.0 +0.4
motility inner cut, LB soft agar motility assay +0.3 +0.1 +0.2 -0.9 -0.2
motility outer cut, LB soft agar motility assay +0.3 +0.1 +0.2 -0.9 -0.1
phage P. simiae OR Antrim MOI 1 +0.4 +0.2 -0.3 -0.9 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 +0.3 -0.0 -0.8 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +0.5 +0.2 +0.2 -1.1 -0.1
phage Wcs_0.1 +0.4 +0.3 +0.1 -0.9 -0.1
motility outer cut, LB soft agar motility assay +0.6 +0.2 +0.0 -0.6 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -0.3 +0.5 -0.5 +0.2
motility inner cut, LB soft agar motility assay +0.4 +0.3 +0.4 -0.8 -0.1
phage P. simiae ORA MOI 0.1 +0.6 +0.3 -0.2 -0.6 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.0 -0.0 -0.4 +0.8 +0.3
seeds Growth on radish seeds for 72 hours -0.2 +0.5 +0.2 +0.9 -0.5
soil soil sample 3; outgrowth in LB +0.8 +0.1 +0.3 -0.4 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.7 +0.3 -0.3 +0.3 -0.0
phage P. simiae OR Antrim MOI 10 -0.3 +0.4 +0.1 +0.2 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4 +0.1 +0.2 +0.9 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.4 -0.1 +0.9 -0.1
rhizosphere rhizosphere sample 1; outgrowth in LB -0.2 +0.7 +0.3 +0.5 -0.1
stress 1-ethyl-3-methylimidazolium chloride 200 mM +0.2 +0.3 -0.2 +0.9 +0.0
seeds Growth on radish seeds for 24 hours +0.7 +0.4 +0.3 -0.1 +0.2
nitrogen source Putrescine (N) +0.3 +0.0 +0.1 +1.1 -0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.2 -0.1 +0.6 +0.5 +0.2
rhizosphere rhizosphere sample 2; outgrowth in LB +0.6 +0.7 +0.5 -0.5 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 +0.2 +0.2 +1.3 +0.1
stress LB_noSalt with Chloride 1100 mM +0.3 +0.3 +0.4 +0.6 +0.3
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