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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_00340 and PS417_00345 overlap by 4 nucleotides
PS417_00345 and PS417_00350 are separated by 161 nucleotides
PS417_00350 and PS417_00355 are separated by 51 nucleotides
PS417_00355 and PS417_00360 overlap by 1 nucleotides
PS417_00340: PS417_00340 - protoheme IX farnesyltransferase, at 66,005 to 66,904
_00340
PS417_00345: PS417_00345 - copper-binding protein, at 66,901 to 67,536
_00345
PS417_00350: PS417_00350 - methionine ABC transporter substrate-binding protein, at 67,698 to 68,468
_00350
PS417_00355: PS417_00355 - metal ABC transporter permease, at 68,520 to 69,194
_00355
PS417_00360: PS417_00360 - methionine ABC transporter ATP-binding protein, at 69,194 to 70,201
_00360
Group
Condition
PS417
_00340
PS417
_00345
PS417
_00350
PS417
_00355
PS417
_00360
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.6
-1.7
-0.2
-3.6
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
+0.5
-1.1
-3.4
N.D.
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
+0.2
-4.2
+0.7
-0.0
N.D.
motility_chemotaxis
Method=Plug_approach; Chemical=None; Chemical_conc=0mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.2
-2.7
-0.2
-0.2
N.D.
liquid stress
Fraxetin 3 mM; liquid stress
-0.0
-3.0
-0.1
+0.0
N.D.
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.4
-2.3
-0.3
-0.1
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.5
-2.2
-0.5
+0.0
N.D.
phage
Wcs_0.1
+0.4
-1.1
-0.3
-1.8
N.D.
carbon source
L-Valine (C)
+0.2
-0.0
-0.1
-2.8
N.D.
liquid stress
Scopoletin 3 mM; liquid stress
-0.1
-2.7
-0.1
+0.2
N.D.
soil
soil sample 3; outgrowth in LB
-0.1
+0.2
+0.1
-2.8
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.0
-3.2
+0.3
+0.6
N.D.
solid stress
Fraxetin 3 mM; solid stress
+0.1
-3.2
+0.3
+0.9
N.D.
liquid stress
Fraxetin 2 mM; liquid stress
-0.2
-2.2
-0.2
+0.8
N.D.
liquid stress
Fraxetin 3 mM; liquid stress
+0.0
-2.3
+0.3
+0.2
N.D.
solid stress
80% Methanol 0.5 mM; solid stress
-0.1
-2.4
-0.0
+0.8
N.D.
solid stress
Fraxetin 3 mM; solid stress
-0.2
-2.5
+0.7
+0.3
N.D.
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.5
-2.2
-0.2
+1.4
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
-2.0
-0.4
+0.5
N.D.
seeds
Growth on radish seeds for 72 hours
+0.0
+0.8
-0.8
-1.3
N.D.
phage
P. simiae OR Antrim MOI 1
+0.3
+0.8
-0.5
-1.8
N.D.
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
+0.4
-1.7
+0.6
-0.5
N.D.
soil
soil sample 5; outgrowth in LB
-0.5
+1.0
-0.5
-1.1
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
-0.2
-1.5
+0.1
+0.9
N.D.
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
+0.1
-2.2
+0.5
+1.0
N.D.
phage
JP1 MOI 0.1
+0.8
-0.8
-0.3
+0.8
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.7
+1.6
-0.6
-1.1
N.D.
phage
P. simiae Grant Run MOI 0.1
-0.0
-0.5
-0.5
+2.2
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
+1.5
-0.8
+0.7
N.D.
root
root sample 6; outgrowth in LB
+0.2
+2.2
+0.2
-0.9
N.D.
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