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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_03340 and PS417_03345 are separated by 242 nucleotides
PS417_03345 and PS417_03350 are separated by 18 nucleotides
PS417_03350 and PS417_03355 are separated by 18 nucleotides
PS417_03355 and PS417_03360 overlap by 77 nucleotides
PS417_03340: PS417_03340 - gluconate transporter, at 765,701 to 767,071
_03340
PS417_03345: PS417_03345 - type I secretion protein TolC, at 767,314 to 768,534
_03345
PS417_03350: PS417_03350 - cytochrome C peroxidase, at 768,553 to 769,659
_03350
PS417_03355: PS417_03355 - cation transporter, at 769,678 to 772,815
_03355
PS417_03360: PS417_03360 - histidine kinase, at 772,739 to 774,175
_03360
Group
Condition
PS417
_03340
PS417
_03345
PS417
_03350
PS417
_03355
PS417
_03360
carbon source
L-Valine (C)
-0.3
-1.2
-1.5
+0.2
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.6
-0.3
+0.2
+0.1
-1.3
soil
soil sample 2; outgrowth in LB
-0.5
-1.2
+0.1
-0.5
-0.4
supernatant
Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
-0.6
-0.3
-0.5
-0.3
+0.2
agar plate interaction control
Taped volatile agar plate with no fungus
-0.5
+0.5
-1.8
+0.2
+0.1
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.6
-0.4
+0.4
-0.3
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-1.0
-0.2
-0.2
+0.3
+0.2
seeds
Growth on radish seeds for 72 hours
+0.6
-0.4
-0.6
-0.2
-0.3
soil
soil sample 7; outgrowth in LB
-0.3
+0.6
-0.7
-0.4
-0.1
phage
JP1 MOI 10
-1.3
-0.0
+0.4
-0.2
+0.3
phage
P. simiae ORA MOI 0.1
+0.4
+0.1
-1.5
+0.4
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.7
-0.5
+0.2
-0.3
+0.6
phage
Wcs_1
-1.4
+0.1
+0.3
+0.2
+0.2
nophagecontrol
Only library
-1.0
-0.3
+0.3
+0.3
+0.3
carbon source
L-Valine (C)
+0.9
-0.1
-0.5
-0.1
-0.3
phage
P. simiae OR Antrim MOI 0.1
+1.1
-0.4
-1.0
-0.1
+0.2
soil
soil sample 5; outgrowth in LB
+0.5
+0.1
+0.7
-0.2
-1.1
phage
JP1 MOI 0.1
-0.5
+0.4
+0.8
-0.8
+0.1
solid stress
Fraxetin 3 mM; solid stress
+0.9
-0.5
+0.2
-0.5
+0.0
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.3
+1.0
-0.8
+0.2
-0.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.9
+0.5
-0.1
+0.2
+0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.1
-0.7
-0.0
+0.9
+0.2
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
+0.5
-0.7
+0.4
+0.3
-0.1
seeds
Growth on radish seeds for 72 hours
+1.1
+0.9
-0.9
-0.1
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
+0.6
-0.6
+0.6
+0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.7
-0.7
+0.3
+0.7
+0.6
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.3
-0.5
+0.9
-0.1
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.7
-0.2
+0.4
-0.0
+0.9
carbon source
Carbon source L-Citrulline 5 mM
+0.4
+0.6
+0.4
-0.1
+0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.7
-0.1
-0.2
+0.7
+1.0
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