Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_03245 and PS417_03250 are separated by 39 nucleotidesPS417_03250 and PS417_03255 are separated by 502 nucleotidesPS417_03255 and PS417_03260 are separated by 139 nucleotidesPS417_03260 and PS417_03265 are separated by 158 nucleotides PS417_03245: PS417_03245 - omega amino acid--pyruvate aminotransferase, at 741,039 to 742,388 _03245 PS417_03250: PS417_03250 - methylmalonate-semialdehyde dehydrogenase, at 742,428 to 743,924 _03250 PS417_03255: PS417_03255 - 23S ribosomal RNA, at 744,427 to 747,342 _03255 PS417_03260: PS417_03260 - 5S ribosomal RNA, at 747,482 to 747,597 _03260 PS417_03265: PS417_03265 - paraquat-inducible protein A, at 747,756 to 748,415 _03265
Group Condition PS417_03245 PS417_03250 PS417_03255 PS417_03260 PS417_03265
nitrogen source Uridine (N) -3.6 -0.3 N.D. -0.1 +0.1
soil soil sample 8; outgrowth in LB -0.4 -0.2 N.D. -3.2 +0.2
solid stress 80% Methanol 0.5 mM; solid stress -0.2 -0.0 N.D. -3.0 -0.2
rhizosphere rhizosphere sample 8; outgrowth in LB -0.3 -0.2 N.D. -2.6 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.1 +0.1 N.D. -3.4 -0.1
solid stress 80% Methanol 3 mM; solid stress -0.2 -0.0 N.D. -3.0 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -0.1 N.D. -3.5 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +0.2 N.D. -3.3 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 +0.0 N.D. -3.5 -0.0
soil soil sample 4; outgrowth in LB -0.7 -0.0 N.D. -3.1 +0.5
agar plate interaction control Taped volatile agar plate with no fungus -0.3 +0.1 N.D. -3.3 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 +0.0 N.D. -3.5 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -0.2 N.D. -3.4 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Threonine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.0 +0.1 N.D. -3.4 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.0 +0.1 N.D. -3.4 +0.1
solid stress Scopoletin 0.5 mM; solid stress +0.2 -0.1 N.D. -3.1 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.0 +0.2 N.D. -3.3 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 +0.1 N.D. -3.4 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +0.1 N.D. -3.0 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 +0.0 N.D. -3.4 +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +0.3 N.D. -3.0 +0.0
solid stress Scopoletin 2 mM; solid stress +0.1 -0.0 N.D. -3.0 +0.2
supernatant control Vogels_fungal_media 0.1X +0.1 +0.4 N.D. -2.6 -0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.2 -0.0 N.D. -2.8 +0.7
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.2 -0.0 N.D. -2.8 +1.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.4 +0.1 N.D. -2.7 +1.2
stress Nalidixic 0.025 mg/ml +0.1 +0.1 N.D. +2.7 -0.6
stress Doxycycline hyclate 0.001 mg/ml +0.2 +0.2 N.D. +2.9 -0.8
stress Neomycin 0.04 mg/ml +0.1 -0.1 N.D. +3.0 +2.6
stress Gentamicin 0.008 mg/ml -0.1 +0.1 N.D. +6.7 +0.6
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