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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_03245 and PS417_03250 are separated by 39 nucleotides
PS417_03250 and PS417_03255 are separated by 502 nucleotides
PS417_03255 and PS417_03260 are separated by 139 nucleotides
PS417_03260 and PS417_03265 are separated by 158 nucleotides
PS417_03245: PS417_03245 - omega amino acid--pyruvate aminotransferase, at 741,039 to 742,388
_03245
PS417_03250: PS417_03250 - methylmalonate-semialdehyde dehydrogenase, at 742,428 to 743,924
_03250
PS417_03255: PS417_03255 - 23S ribosomal RNA, at 744,427 to 747,342
_03255
PS417_03260: PS417_03260 - 5S ribosomal RNA, at 747,482 to 747,597
_03260
PS417_03265: PS417_03265 - paraquat-inducible protein A, at 747,756 to 748,415
_03265
Group
Condition
PS417
_03245
PS417
_03250
PS417
_03255
PS417
_03260
PS417
_03265
nitrogen source
Uridine (N)
-3.6
-0.3
N.D.
-0.1
+0.1
soil
soil sample 8; outgrowth in LB
-0.4
-0.2
N.D.
-3.2
+0.2
solid stress
80% Methanol 0.5 mM; solid stress
-0.2
-0.0
N.D.
-3.0
-0.2
rhizosphere
rhizosphere sample 8; outgrowth in LB
-0.3
-0.2
N.D.
-2.6
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.1
+0.1
N.D.
-3.4
-0.1
solid stress
80% Methanol 3 mM; solid stress
-0.2
-0.0
N.D.
-3.0
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.1
N.D.
-3.5
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.1
+0.2
N.D.
-3.3
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
+0.0
N.D.
-3.5
-0.0
soil
soil sample 4; outgrowth in LB
-0.7
-0.0
N.D.
-3.1
+0.5
agar plate interaction control
Taped volatile agar plate with no fungus
-0.3
+0.1
N.D.
-3.3
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
+0.0
N.D.
-3.5
+0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
-0.2
N.D.
-3.4
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Threonine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.0
+0.1
N.D.
-3.4
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.0
+0.1
N.D.
-3.4
+0.1
solid stress
Scopoletin 0.5 mM; solid stress
+0.2
-0.1
N.D.
-3.1
-0.1
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB
-0.0
+0.2
N.D.
-3.3
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
+0.1
N.D.
-3.4
+0.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.2
+0.1
N.D.
-3.0
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
+0.0
N.D.
-3.4
+0.7
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.2
+0.3
N.D.
-3.0
+0.0
solid stress
Scopoletin 2 mM; solid stress
+0.1
-0.0
N.D.
-3.0
+0.2
supernatant control
Vogels_fungal_media 0.1X
+0.1
+0.4
N.D.
-2.6
-0.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.2
-0.0
N.D.
-2.8
+0.7
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.2
-0.0
N.D.
-2.8
+1.0
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.4
+0.1
N.D.
-2.7
+1.2
stress
Nalidixic 0.025 mg/ml
+0.1
+0.1
N.D.
+2.7
-0.6
stress
Doxycycline hyclate 0.001 mg/ml
+0.2
+0.2
N.D.
+2.9
-0.8
stress
Neomycin 0.04 mg/ml
+0.1
-0.1
N.D.
+3.0
+2.6
stress
Gentamicin 0.008 mg/ml
-0.1
+0.1
N.D.
+6.7
+0.6
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