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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_03020 and PS417_03025 are separated by 251 nucleotides
PS417_03025 and PS417_03030 are separated by 151 nucleotides
PS417_03030 and PS417_03035 overlap by 8 nucleotides
PS417_03035 and PS417_03040 are separated by 24 nucleotides
PS417_03020: PS417_03020 - MFS transporter, at 695,355 to 695,648
_03020
PS417_03025: PS417_03025 - penicillin-binding protein 1C, at 695,900 to 698,191
_03025
PS417_03030: PS417_03030 - pyrophosphatase, at 698,343 to 698,651
_03030
PS417_03035: PS417_03035 - SAM-dependent methyltransferase, at 698,644 to 699,393
_03035
PS417_03040: PS417_03040 - hypothetical protein, at 699,418 to 700,020
_03040
Group
Condition
PS417
_03020
PS417
_03025
PS417
_03030
PS417
_03035
PS417
_03040
rhizosphere
rhizosphere sample 2; outgrowth in LB
N.D.
+0.1
-0.7
-0.1
-0.6
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
+0.1
-0.6
-0.4
-0.2
seeds
Growth on radish seeds for 72 hours
N.D.
-0.4
-0.4
+0.1
-0.5
supernatant
Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
N.D.
-0.6
-0.4
+0.0
-0.2
root
root sample 6; outgrowth in LB
N.D.
-0.3
+0.1
-0.4
-0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+0.1
-0.8
+0.3
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.5
+0.3
+0.0
-0.5
soil
soil sample 8; outgrowth in LB
N.D.
-0.3
+0.4
-0.4
-0.3
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
+0.5
-0.5
-0.2
-0.3
solid stress
Fraxetin 2 mM; solid stress
N.D.
+0.5
-0.7
-0.2
+0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.4
-0.4
+0.3
+0.1
phage
Wcs_1
N.D.
-0.3
-0.5
+0.1
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.5
-0.6
-0.1
-0.0
soil
soil sample 5; outgrowth in LB
N.D.
-0.5
+0.5
-0.3
-0.0
supernatant
LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
N.D.
-0.5
+0.5
+0.0
-0.2
seeds
Growth on radish seeds for 72 hours
N.D.
+0.2
+0.4
-0.2
-0.5
phage
P. simiae Grant Run MOI 10
N.D.
+0.3
+0.3
-0.1
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.1
+0.7
-0.5
-0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
-0.5
+0.4
+0.2
-0.1
supernatant control
Vogels_fungal_media 0.1X
N.D.
-0.3
+0.5
-0.3
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
N.D.
+0.5
+0.3
-0.3
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
+0.4
-0.4
-0.1
+0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
N.D.
-0.3
+0.7
+0.0
+0.2
supernatant control
Vogels_fungal_media 0.1X
N.D.
-0.2
+0.9
+0.1
-0.1
phage
JP1 MOI 1
N.D.
-0.2
+0.7
+0.0
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.3
-0.1
+0.2
+0.6
carbon source
2'-Deoxyinosine 5 mM (C)
N.D.
+0.4
+0.6
-0.1
+0.1
nophagecontrol
TIme6-nophage
N.D.
+0.2
+0.7
-0.2
+0.2
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+0.3
+1.0
-0.0
-0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+0.7
-0.3
+0.2
+0.6
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