Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_03020 and PS417_03025 are separated by 251 nucleotidesPS417_03025 and PS417_03030 are separated by 151 nucleotidesPS417_03030 and PS417_03035 overlap by 8 nucleotidesPS417_03035 and PS417_03040 are separated by 24 nucleotides PS417_03020: PS417_03020 - MFS transporter, at 695,355 to 695,648 _03020 PS417_03025: PS417_03025 - penicillin-binding protein 1C, at 695,900 to 698,191 _03025 PS417_03030: PS417_03030 - pyrophosphatase, at 698,343 to 698,651 _03030 PS417_03035: PS417_03035 - SAM-dependent methyltransferase, at 698,644 to 699,393 _03035 PS417_03040: PS417_03040 - hypothetical protein, at 699,418 to 700,020 _03040
Group Condition PS417_03020 PS417_03025 PS417_03030 PS417_03035 PS417_03040
rhizosphere rhizosphere sample 2; outgrowth in LB N.D. +0.1 -0.7 -0.1 -0.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +0.1 -0.6 -0.4 -0.2
seeds Growth on radish seeds for 72 hours N.D. -0.4 -0.4 +0.1 -0.5
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x N.D. -0.6 -0.4 +0.0 -0.2
root root sample 6; outgrowth in LB N.D. -0.3 +0.1 -0.4 -0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.1 -0.8 +0.3 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.5 +0.3 +0.0 -0.5
soil soil sample 8; outgrowth in LB N.D. -0.3 +0.4 -0.4 -0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +0.5 -0.5 -0.2 -0.3
solid stress Fraxetin 2 mM; solid stress N.D. +0.5 -0.7 -0.2 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.4 -0.4 +0.3 +0.1
phage Wcs_1 N.D. -0.3 -0.5 +0.1 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.5 -0.6 -0.1 -0.0
soil soil sample 5; outgrowth in LB N.D. -0.5 +0.5 -0.3 -0.0
supernatant LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x N.D. -0.5 +0.5 +0.0 -0.2
seeds Growth on radish seeds for 72 hours N.D. +0.2 +0.4 -0.2 -0.5
phage P. simiae Grant Run MOI 10 N.D. +0.3 +0.3 -0.1 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.1 +0.7 -0.5 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.5 +0.4 +0.2 -0.1
supernatant control Vogels_fungal_media 0.1X N.D. -0.3 +0.5 -0.3 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.5 +0.3 -0.3 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.4 -0.4 -0.1 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -0.3 +0.7 +0.0 +0.2
supernatant control Vogels_fungal_media 0.1X N.D. -0.2 +0.9 +0.1 -0.1
phage JP1 MOI 1 N.D. -0.2 +0.7 +0.0 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.3 -0.1 +0.2 +0.6
carbon source 2'-Deoxyinosine 5 mM (C) N.D. +0.4 +0.6 -0.1 +0.1
nophagecontrol TIme6-nophage N.D. +0.2 +0.7 -0.2 +0.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.3 +1.0 -0.0 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.7 -0.3 +0.2 +0.6
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