Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_03010 and PS417_03015 are separated by 178 nucleotidesPS417_03015 and PS417_03020 are separated by 99 nucleotidesPS417_03020 and PS417_03025 are separated by 251 nucleotidesPS417_03025 and PS417_03030 are separated by 151 nucleotides PS417_03010: PS417_03010 - XRE family transcriptional regulator, at 688,835 to 690,178 _03010 PS417_03015: PS417_03015 - hypothetical protein, at 690,357 to 695,255 _03015 PS417_03020: PS417_03020 - MFS transporter, at 695,355 to 695,648 _03020 PS417_03025: PS417_03025 - penicillin-binding protein 1C, at 695,900 to 698,191 _03025 PS417_03030: PS417_03030 - pyrophosphatase, at 698,343 to 698,651 _03030
Group Condition PS417_03010 PS417_03015 PS417_03020 PS417_03025 PS417_03030
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -0.4 N.D. -0.4 -0.4
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2 -0.3 N.D. -0.5 -0.4
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x +0.1 -0.3 N.D. -0.6 -0.4
phage P. simiae Grant Run MOI 0.1 +0.2 -0.3 N.D. -0.2 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 -0.5 N.D. -0.4 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -0.6 N.D. -0.5 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +0.2 N.D. +0.1 -0.8
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 +0.3 N.D. -0.4 -0.6
supernatant control Vogels_fungal_media 0.1X -0.5 -0.2 N.D. -0.3 +0.5
soil soil sample 5; outgrowth in LB -0.0 -0.3 N.D. -0.5 +0.5
supernatant LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x -0.0 -0.3 N.D. -0.5 +0.5
rhizosphere rhizosphere sample 8; outgrowth in LB +0.7 -0.2 N.D. -0.5 -0.1
phage JP1 MOI 1 -0.5 -0.0 N.D. -0.2 +0.7
phage JP1 MOI 0.1 -0.4 +0.2 N.D. -0.4 +0.5
solid stress Fraxetin 2 mM; solid stress -0.1 +0.2 N.D. +0.5 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Phenylalanine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.2 +0.2 N.D. +0.4 -0.5
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.2 +0.3 N.D. +0.5 -0.5
supernatant control Vogels_fungal_media 0.1X -0.4 -0.2 N.D. -0.2 +0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.1 +0.4 N.D. +0.3 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.2 -0.2 N.D. -0.3 +0.7
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.4 -0.1 N.D. +0.1 +0.7
carbon source 2'-Deoxyinosine 5 mM (C) -0.0 +0.3 N.D. +0.6 -0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.3 +0.2 N.D. +0.4 +0.4
solid stress 80% Methanol 2 mM; solid stress -0.3 +0.5 N.D. +0.4 +0.2
carbon source 2'-Deoxyinosine 5 mM (C) +0.3 -0.1 N.D. +0.4 +0.6
solid stress Fraxetin 0.5 mM; solid stress +0.1 +0.4 N.D. +0.5 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.2 +0.2 N.D. +0.4 +0.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 +0.1 N.D. +0.3 +1.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.4 N.D. +0.2 +0.6
rhizosphere rhizosphere sample 1; outgrowth in LB +0.5 +0.4 N.D. +0.6 +0.4
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