Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_02990 and PS417_02995 are separated by 2 nucleotidesPS417_02995 and PS417_03000 are separated by 182 nucleotidesPS417_03000 and PS417_03005 are separated by 142 nucleotidesPS417_03005 and PS417_03010 are separated by 131 nucleotides PS417_02990: PS417_02990 - hypothetical protein, at 684,487 to 685,554 _02990 PS417_02995: PS417_02995 - NUDIX hydrolase, at 685,557 to 686,093 _02995 PS417_03000: PS417_03000 - translation initiation factor Sui1, at 686,276 to 686,647 _03000 PS417_03005: PS417_03005 - arginine decarboxylase, at 686,790 to 688,703 _03005 PS417_03010: PS417_03010 - XRE family transcriptional regulator, at 688,835 to 690,178 _03010
Group Condition PS417_02990 PS417_02995 PS417_03000 PS417_03005 PS417_03010
solid stress Fraxetin 3 mM; solid stress -4.9 -0.4 -1.9 -0.4 -0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.1 -0.4 -1.4 -1.6 -0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.8 -0.4 -1.5 -1.4 -0.1
soil soil sample 3; outgrowth in LB -3.0 -0.4 -0.2 -1.1 +0.5
soil soil sample 5; outgrowth in LB -0.3 -0.5 -1.7 -1.0 -0.0
soil soil sample 6; outgrowth in LB -2.1 -0.2 -0.7 -0.1 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days -0.4 -0.2 -0.9 -1.5 -0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.2 -0.0 -1.7 -1.1 +0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 -1.0 -1.2 -0.6 -0.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.4 -0.4 -0.4 -1.9 +0.1
solid stress Fraxetin 3 mM; solid stress -1.5 -0.1 -0.6 -0.6 -0.0
stress methylglyoxal 0.032 vol% -0.5 -1.0 -0.4 -0.8 -0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.7 +0.2 -1.1 -1.1 +0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.2 -1.0 -1.3 -0.3 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 -0.0 -1.5 -0.5 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.8 -1.1 +0.2 -0.5
soil soil sample 8; outgrowth in LB -0.3 +0.4 -1.2 -0.9 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -0.8 -1.7 +0.1 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 -0.7 -0.3 -1.0 -0.1
soil soil sample 7; outgrowth in LB +0.5 -0.0 -1.0 -1.1 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.7 -0.5 -0.9 -0.7 -0.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.4 +0.5 -0.3 -1.9 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 +0.5 -0.6 -1.0 -0.4
motility inner cut, LB soft agar motility assay +0.2 +0.6 -0.9 -1.0 +0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.5 +0.3 -0.9 -1.0 +0.2
soil soil sample 4; outgrowth in LB -1.2 +0.7 +0.7 -1.2 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +1.0 +0.7 -0.4 -0.6 -0.1
phage P. simiae OR Antrim MOI 10 +1.3 +1.0 -0.4 -0.5 +0.2
root root sample 6; outgrowth in LB +1.4 +0.5 -0.3 -0.3 +0.6
stress Gentamicin 0.008 mg/ml +0.1 +0.1 +1.2 +1.5 -0.2
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