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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_02990 and PS417_02995 are separated by 2 nucleotides
PS417_02995 and PS417_03000 are separated by 182 nucleotides
PS417_03000 and PS417_03005 are separated by 142 nucleotides
PS417_03005 and PS417_03010 are separated by 131 nucleotides
PS417_02990: PS417_02990 - hypothetical protein, at 684,487 to 685,554
_02990
PS417_02995: PS417_02995 - NUDIX hydrolase, at 685,557 to 686,093
_02995
PS417_03000: PS417_03000 - translation initiation factor Sui1, at 686,276 to 686,647
_03000
PS417_03005: PS417_03005 - arginine decarboxylase, at 686,790 to 688,703
_03005
PS417_03010: PS417_03010 - XRE family transcriptional regulator, at 688,835 to 690,178
_03010
Group
Condition
PS417
_02990
PS417
_02995
PS417
_03000
PS417
_03005
PS417
_03010
solid stress
Fraxetin 3 mM; solid stress
-4.9
-0.4
-1.9
-0.4
-0.2
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-1.1
-0.4
-1.4
-1.6
-0.3
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.8
-0.4
-1.5
-1.4
-0.1
soil
soil sample 3; outgrowth in LB
-3.0
-0.4
-0.2
-1.1
+0.5
soil
soil sample 5; outgrowth in LB
-0.3
-0.5
-1.7
-1.0
-0.0
soil
soil sample 6; outgrowth in LB
-2.1
-0.2
-0.7
-0.1
-0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days
-0.4
-0.2
-0.9
-1.5
-0.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.2
-0.0
-1.7
-1.1
+0.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
-1.0
-1.2
-0.6
-0.0
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.4
-0.4
-0.4
-1.9
+0.1
solid stress
Fraxetin 3 mM; solid stress
-1.5
-0.1
-0.6
-0.6
-0.0
stress
methylglyoxal 0.032 vol%
-0.5
-1.0
-0.4
-0.8
-0.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.7
+0.2
-1.1
-1.1
+0.0
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
+0.2
-1.0
-1.3
-0.3
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
-0.0
-1.5
-0.5
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-0.8
-1.1
+0.2
-0.5
soil
soil sample 8; outgrowth in LB
-0.3
+0.4
-1.2
-0.9
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
-0.8
-1.7
+0.1
+0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.7
-0.7
-0.3
-1.0
-0.1
soil
soil sample 7; outgrowth in LB
+0.5
-0.0
-1.0
-1.1
+0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.7
-0.5
-0.9
-0.7
-0.0
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.4
+0.5
-0.3
-1.9
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.4
+0.5
-0.6
-1.0
-0.4
motility
inner cut, LB soft agar motility assay
+0.2
+0.6
-0.9
-1.0
+0.3
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.5
+0.3
-0.9
-1.0
+0.2
soil
soil sample 4; outgrowth in LB
-1.2
+0.7
+0.7
-1.2
+0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+1.0
+0.7
-0.4
-0.6
-0.1
phage
P. simiae OR Antrim MOI 10
+1.3
+1.0
-0.4
-0.5
+0.2
root
root sample 6; outgrowth in LB
+1.4
+0.5
-0.3
-0.3
+0.6
stress
Gentamicin 0.008 mg/ml
+0.1
+0.1
+1.2
+1.5
-0.2
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