Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_02980 and PS417_02985 are separated by 53 nucleotidesPS417_02985 and PS417_02990 are separated by 298 nucleotidesPS417_02990 and PS417_02995 are separated by 2 nucleotidesPS417_02995 and PS417_03000 are separated by 182 nucleotides PS417_02980: PS417_02980 - chemotaxis protein, at 680,503 to 682,464 _02980 PS417_02985: PS417_02985 - diguanylate cyclase, at 682,518 to 684,188 _02985 PS417_02990: PS417_02990 - hypothetical protein, at 684,487 to 685,554 _02990 PS417_02995: PS417_02995 - NUDIX hydrolase, at 685,557 to 686,093 _02995 PS417_03000: PS417_03000 - translation initiation factor Sui1, at 686,276 to 686,647 _03000
Group Condition PS417_02980 PS417_02985 PS417_02990 PS417_02995 PS417_03000
solid stress Fraxetin 3 mM; solid stress +0.6 -0.1 -4.9 -0.4 -1.9
soil soil sample 5; outgrowth in LB -2.0 -0.0 -0.3 -0.5 -1.7
soil soil sample 3; outgrowth in LB -1.3 +0.6 -3.0 -0.4 -0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml -2.6 -0.0 -0.7 +0.2 -1.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.7 +0.3 -0.2 -1.0 -1.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.4 +0.4 +0.2 -0.8 -1.7
soil soil sample 7; outgrowth in LB -3.0 +0.4 +0.5 -0.0 -1.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.2 +0.2 -1.1 -0.4 -1.4
soil soil sample 6; outgrowth in LB +0.5 +0.2 -2.1 -0.2 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.0 +0.5 -0.2 -0.2 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.2 +1.7 -1.0 -0.5 -0.1
motility inner cut, LB soft agar motility assay -1.7 +0.4 +0.2 +0.6 -0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.0 +1.4 +0.3 -0.8 -1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.1 +1.6 -0.2 -0.0 -0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.8 +0.7 +0.0 -0.3 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.1 +1.4 +0.4 -0.0 -1.5
ecofab LB in EcoFAB_3.5mL +1.4 -1.1 -0.1 -0.2 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.8 +1.9 +0.2 -0.7 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.2 +1.1 +0.1 -0.8 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.5 +1.4 +0.3 +0.5 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 +1.5 -0.5 +0.3 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.4 +0.8 +0.5 -0.9 -0.3
carbon source L-Valine (C) +1.9 +0.2 +0.4 -1.1 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.1 +1.3 -0.6 -0.0 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.7 +0.3 +0.4 +0.5 -0.6
phage P. simiae OR Antrim MOI 10 -0.6 +1.3 +1.3 +1.0 -0.4
agar plate interaction control Taped volatile agar plate with no fungus +1.8 +0.5 +0.9 +0.2 -0.4
phage P. simiae Grant Run MOI 10 +0.8 +1.3 +0.6 +0.4 +0.2
phage P. simiae Grant Run MOI 10 +1.0 +1.7 +1.0 +0.5 -0.3
phage JP1 MOI 10 +2.2 +1.3 +0.1 +0.8 -0.1
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