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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_02980 and PS417_02985 are separated by 53 nucleotides
PS417_02985 and PS417_02990 are separated by 298 nucleotides
PS417_02990 and PS417_02995 are separated by 2 nucleotides
PS417_02995 and PS417_03000 are separated by 182 nucleotides
PS417_02980: PS417_02980 - chemotaxis protein, at 680,503 to 682,464
_02980
PS417_02985: PS417_02985 - diguanylate cyclase, at 682,518 to 684,188
_02985
PS417_02990: PS417_02990 - hypothetical protein, at 684,487 to 685,554
_02990
PS417_02995: PS417_02995 - NUDIX hydrolase, at 685,557 to 686,093
_02995
PS417_03000: PS417_03000 - translation initiation factor Sui1, at 686,276 to 686,647
_03000
Group
Condition
PS417
_02980
PS417
_02985
PS417
_02990
PS417
_02995
PS417
_03000
solid stress
Fraxetin 3 mM; solid stress
+0.6
-0.1
-4.9
-0.4
-1.9
soil
soil sample 5; outgrowth in LB
-2.0
-0.0
-0.3
-0.5
-1.7
soil
soil sample 3; outgrowth in LB
-1.3
+0.6
-3.0
-0.4
-0.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-2.6
-0.0
-0.7
+0.2
-1.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.7
+0.3
-0.2
-1.0
-1.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.4
+0.4
+0.2
-0.8
-1.7
soil
soil sample 7; outgrowth in LB
-3.0
+0.4
+0.5
-0.0
-1.0
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.2
+0.2
-1.1
-0.4
-1.4
soil
soil sample 6; outgrowth in LB
+0.5
+0.2
-2.1
-0.2
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-2.0
+0.5
-0.2
-0.2
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-2.2
+1.7
-1.0
-0.5
-0.1
motility
inner cut, LB soft agar motility assay
-1.7
+0.4
+0.2
+0.6
-0.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.0
+1.4
+0.3
-0.8
-1.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-2.1
+1.6
-0.2
-0.0
-0.3
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.8
+0.7
+0.0
-0.3
+0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.1
+1.4
+0.4
-0.0
-1.5
ecofab
LB in EcoFAB_3.5mL
+1.4
-1.1
-0.1
-0.2
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.8
+1.9
+0.2
-0.7
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+1.2
+1.1
+0.1
-0.8
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.5
+1.4
+0.3
+0.5
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
+1.5
-0.5
+0.3
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+1.4
+0.8
+0.5
-0.9
-0.3
carbon source
L-Valine (C)
+1.9
+0.2
+0.4
-1.1
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+1.1
+1.3
-0.6
-0.0
+0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+1.7
+0.3
+0.4
+0.5
-0.6
phage
P. simiae OR Antrim MOI 10
-0.6
+1.3
+1.3
+1.0
-0.4
agar plate interaction control
Taped volatile agar plate with no fungus
+1.8
+0.5
+0.9
+0.2
-0.4
phage
P. simiae Grant Run MOI 10
+0.8
+1.3
+0.6
+0.4
+0.2
phage
P. simiae Grant Run MOI 10
+1.0
+1.7
+1.0
+0.5
-0.3
phage
JP1 MOI 10
+2.2
+1.3
+0.1
+0.8
-0.1
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